The Lipid composition of plasma membrane subfractions originating from the three major functional domains of the rat hepatocyte cell surface

Kremmer, T.; Wisher, M. H.; Evans, William Howard

doi:10.1016/0005-2736(76)90039-0

Cited by 114 publications

(42 citation statements)

References 41 publications

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“…In polarized epithelial cells, the apical surface is even further enriched for these lipid species (158,281,295,597). One possible function of cholesterol and glycosphingolipids is to impart structural rigidity and decreased permeability to the apical domain, which in turn protects the cell against the harsh external environment it faces (e.g., the detergent-like bile or high acidity) (295).…”

Section: Cholesterol and Glycosphingolipidsmentioning

confidence: 99%

Transcytosis: Crossing Cellular Barriers

Tuma¹,

Hubbard

2003

Physiological Reviews

572

493

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Tuma, Pamela L., and Ann L. Hubbard. Transcytosis: Crossing Cellular Barriers. Physiol Rev 83: 871–932, 2003; 10.1152/physrev.00001.2003.—Transcytosis, the vesicular transport of macromolecules from one side of a cell to the other, is a strategy used by multicellular organisms to selectively move material between two environments without altering the unique compositions of those environments. In this review, we summarize our knowledge of the different cell types using transcytosis in vivo, the variety of cargo moved, and the diverse pathways for delivering that cargo. We evaluate in vitro models that are currently being used to study transcytosis. Caveolae-mediated transcytosis by endothelial cells that line the microvasculature and carry circulating plasma proteins to the interstitium is explained in more detail, as is clathrin-mediated transcytosis of IgA by epithelial cells of the digestive tract. The molecular basis of vesicle traffic is discussed, with emphasis on the gaps and uncertainties in our understanding of the molecules and mechanisms that regulate transcytosis. In our view there is still much to be learned about this fundamental process.

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Section: Cholesterol and Glycosphingolipidsmentioning

confidence: 99%

Transcytosis: Crossing Cellular Barriers

Tuma¹,

Hubbard

2003

Physiological Reviews

572

493

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“…14). In a few experiments, phosphatidylcholine alone or with 10 mol% cholesterol was used, or the lipid mixture had the following composition: phosphatidylcholine (46), phosphatidylethanolamine (27), phosphatidylinositol (7), phosphatidylserine (14), and cholesterol (6). Lipids were obtained from Sigma and stored frozen under N2; stock solutions were prepared in chloroform mixed in appropriate ratios, dried under a N2 stream, and stored frozen.…”

Section: Methodsmentioning

confidence: 99%

Isolated liver gap junctions: gating of transjunctional currents is similar to that in intact pairs of rat hepatocytes.

Spray¹,

Sáez²,

Brosius³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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“…PC is the exclusive ( Ͼ 95%) phospholipid in human bile (with an acyl chain composition similar to egg yolk PC) (12). In contrast, both PC and sphingomyelin (SM) are the major phospholipids of the hepatocyte canalicular membrane outer leaflet (13). Cholesterol has a high affinity for SM (14)(15)(16) and is thought to be preferentially located together with this phospholipid in detergent-resistant rafts in the canalicular membrane (17).…”

mentioning

confidence: 99%