The lipid composition of phosphorylating ‘digitonin particles’ and water- and saline-extracted mitochondria from rat liver

Structural lipoproteins of the mucosal cell

“…The combined saline extracts contained 32-5 % of the total nitrogen of the mitochondria and 50-1 % of that of the microsomes. These values are lower than those found by Dallam (1955Dallam ( , 1958 and Bartley, Getz, Notton & Renshaw (1962) for ratliver fractions. Only very small amounts of lipid appeared in these extracts.…”

“…This may be because of difficulty in extraction in the presence of the detergents but is more probably due to breakdown of the phospholipids during the prolonged period required for the various manipulations. Bartley et al (1962) found that hydrolysis of phospholipid occurred during treatment of rat-liver mitochondria with digitonin. Some destruction of 7-dehydrocholesterol also occurred, but, as the total sterol: 7-dehydrocholesterol ratio in the residues obtained with deoxycholate is the same as that of the intact organelles, there is no discrimination between the two sterols by the detergent.…”

The phospholipids of pork muscle, and their relation to the post‐mortem rate of glycolysis

“…This similarity in phospholipid composition (which has been reported for the subcellular fractions of various tissues31) is a result of the association of the phospholipids with well defined structures, the membranes, which, contain the bulk of the cell 32 The membranes of the nuclei, mitochondria, and reticulum (or 'microsomes'), which comprise most of the membranous material of the ~e11,~~,33 consist of lipid with a high proportion of phospholipids in association with protein. In mitochondria, as much as 93-95 % of the lipid consists of phospholipids.27, 34,35 The phospholipids of the mitochondrial cell fraction, which (Table IV) comprise 52 % 'lecithin fraction', 22.5 % 'kephalin fraction', 15 % 'inositide fraction', and 7 % of 'early fraction', may be compared with those reported in the mitochondria of the heart muscle of ox and pig. In pig heart mitochondria30 these components account for 36%, 25%, 14% and 13%, respectively, of the total phospholipids in this cell fraction.…”

Studies on Compounds and Individual Lipids of Wheat Germ

“…Separation of total lipid in polar and nonpolar lipids. To obtain a polar fraction free from nonpolar lipids, a separaticn was done on a silicic acid column (Bartley et al, 1962). A column (20 mm diameter) containing 18 g of silicic acid (Mallinkrodt, 100 mesh, activated at 110°C overnight) was used.…”