The linker histone in Saccharomyces cerevisiae interacts with actin-related protein 4 and both regulate chromatin structure and cellular morphology

Georgieva, Milena; Staneva, Dessislava; Uzunova, Katya; Efremov, Toni; Balashev, Konstantin; Harata, Masahiko; Miloshev, George

doi:10.1016/j.biocel.2014.12.006

Cited by 16 publications

(28 citation statements)

References 59 publications

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“…By binding core histones and histone side-chains, both Arp4 and Arp8 may assist recruitment of various complexes to chromatin, thus regulating remodeling and transcription ( Harata et al, 1999b , 2002 ; Galarneau et al, 2000 ; Shen et al, 2003 ; Downs et al, 2004 ; Gerhold et al, 2012 ; Saravanan et al, 2012 ). In budding yeast, Arp4 has also been shown to bind the linker histone Hho1, a unit key in establishing and organizing higher-order chromatin structure ( Georgieva et al, 2015 ). An Arp4 mutant strain (G187R), which displays only minimal morphological defects, exhibits substantial cellular and nuclear morphological deviation and a dramatic collapse of higher-order chromatin structure upon simultaneous knockout of HHO1 ( Georgieva et al, 2015 ).…”

Section: Functions Of Nuclear Actin-related Proteins (Arps 4–9)mentioning

confidence: 99%

“…In budding yeast, Arp4 has also been shown to bind the linker histone Hho1, a unit key in establishing and organizing higher-order chromatin structure ( Georgieva et al, 2015 ). An Arp4 mutant strain (G187R), which displays only minimal morphological defects, exhibits substantial cellular and nuclear morphological deviation and a dramatic collapse of higher-order chromatin structure upon simultaneous knockout of HHO1 ( Georgieva et al, 2015 ). Indeed, this Arp4 - HHO1 double mutant disrupts chromatin loop organization, weakens chromatin compaction, and exhibits excessively swollen nuclei ( Georgieva et al, 2015 ).…”

Section: Functions Of Nuclear Actin-related Proteins (Arps 4–9)mentioning

confidence: 99%

“…An Arp4 mutant strain (G187R), which displays only minimal morphological defects, exhibits substantial cellular and nuclear morphological deviation and a dramatic collapse of higher-order chromatin structure upon simultaneous knockout of HHO1 ( Georgieva et al, 2015 ). Indeed, this Arp4 - HHO1 double mutant disrupts chromatin loop organization, weakens chromatin compaction, and exhibits excessively swollen nuclei ( Georgieva et al, 2015 ). Moreover, as Arp4 inhibits actin filament assembly in vitro , it may serve as a factor regulating the monomeric state of nuclear actin overall ( Fenn et al, 2011 ).…”

Section: Functions Of Nuclear Actin-related Proteins (Arps 4–9)mentioning

confidence: 99%

“…In binding linker DNA, INO80 assists in uniformly spacing nucleosomes ( Udugama et al, 2011 ). While the overall significance of INO80 binding with linker DNA is still not entirely clear, this interaction may also be involved in fostering proper chromatin architecture and higher-order chromatin structure ( Varga-Weisz and Becker, 2006 ; Georgieva et al, 2015 ).…”

Section: Ino80mentioning

confidence: 99%

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The Nature of Actin-Family Proteins in Chromatin-Modifying Complexes

et al. 2018

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Actin is not only one of the most abundant proteins in eukaryotic cells, but also one of the most versatile. In addition to its familiar involvement in enabling contraction and establishing cellular motility and scaffolding in the cytosol, actin has well-documented roles in a variety of processes within the confines of the nucleus, such as transcriptional regulation and DNA repair. Interestingly, monomeric actin as well as actin-related proteins (Arps) are found as stoichiometric subunits of a variety of chromatin remodeling complexes and histone acetyltransferases, raising the question of precisely what roles they serve in these contexts. Actin and Arps are present in unique combinations in chromatin modifiers, helping to establish structural integrity of the complex and enabling a wide range of functions, such as recruiting the complex to nucleosomes to facilitate chromatin remodeling and promoting ATPase activity of the catalytic subunit. Actin and Arps are also thought to help modulate chromatin dynamics and maintain higher-order chromatin structure. Moreover, the presence of actin and Arps in several chromatin modifiers is necessary for promoting genomic integrity and an effective DNA damage response. In this review, we discuss the involvement of actin and Arps in these nuclear complexes that control chromatin remodeling and histone modifications, while also considering avenues for future study to further shed light on their functional importance.

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Section: Functions Of Nuclear Actin-related Proteins (Arps 4–9)mentioning

confidence: 99%

Section: Functions Of Nuclear Actin-related Proteins (Arps 4–9)mentioning

confidence: 99%

Section: Functions Of Nuclear Actin-related Proteins (Arps 4–9)mentioning

confidence: 99%

Section: Ino80mentioning

confidence: 99%

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The Nature of Actin-Family Proteins in Chromatin-Modifying Complexes

et al. 2018

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“…Hence, we aimed at examining the different molecular mechanisms of cellular response to UVA/B light stress during the process of chronological aging in yeast cells with different chromatin backgrounds. For this purpose, we have used three chromatin mutants—cells that lack the gene for the linker histone, a strain that has a point mutation in ARP4 (the actin-related protein 4) gene and a double mutant with the two mutations induced at the same time [ 33 ] and compared their ability to survive UVA/B stress. Moreover, we have studied the fine molecular mechanisms that underlie the way cells chronologically age under normal and UVA/B stress conditions regarding their cell proliferation, gene expression and chromatin organization.…”

Section: Introductionmentioning

confidence: 99%

Changes in Chromatin Organization Eradicate Cellular Stress Resilience to UVA/B Light and Induce Premature Aging

Vasileva

Staneva

Krasteva

et al. 2021

Cells

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Complex interactions among DNA and nuclear proteins maintain genome organization and stability. The nuclear proteins, particularly the histones, organize, compact, and preserve the stability of DNA, but also allow its dynamic reorganization whenever the nuclear processes require access to it. Five histone classes exist and they are evolutionarily conserved among eukaryotes. The linker histones are the fifth class and over time, their role in chromatin has been neglected. Linker histones interact with DNA and the other histones and thus sustain genome stability and nuclear organization. Saccharomyces cerevisiae is a brilliant model for studying linker histones as the gene for it is a single-copy and is non-essential. We, therefore, created a linker histone-free yeast strain using a knockout of the relevant gene and traced the way cells age chronologically. Here we present our results demonstrating that the altered chromatin dynamics during the chronological lifespan of the yeast cells with a mutation in ARP4 (the actin-related protein 4) and without the gene HHO1 for the linker histone leads to strong alterations in the gene expression profiles of a subset of genes involved in DNA repair and autophagy. The obtained results further prove that the yeast mutants have reduced survival upon UVA/B irradiation possibly due to the accelerated decompaction of chromatin and impaired proliferation. Our hypothesis posits that the higher-order chromatin structure and the interactions among chromatin proteins are crucial for the maintenance of chromatin organization during chronological aging under optimal and UVA-B stress conditions.

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