The Linkage Between Antibiotic and Disinfectant Resistance

Staats, Gunther J.; Carlie, Samantha J. Mc; Walt, B. Van der; Bragg, R.R.

doi:10.1007/978-3-031-23796-6_11

Cited by 3 publications

(2 citation statements)

References 209 publications

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“…However, the overuse of disinfectants can lead to the selection of disinfectant-resistant bacteria, which can then spread and cause infections. The presence of disinfectant resistance genes in K. pneumoniae ST16 isolates highlights the need for appropriate disinfection practices and the importance of surveillance programs for monitoring disinfectant resistance [24,25] Another signi cant nding is the presence of the blaNDM-1 gene, which confers resistance to carbapenems, the last-resort antibiotics used for treating severe infections caused by multidrug-resistant bacteria. Carbapenem resistance is a global threat, and the spread of NDM-1 has been reported in many countries, leading to limited treatment options and increased mortality rates.…”

Section: Discussionmentioning

confidence: 99%

Cracking the Code of Antibiotic Resistance OF Klebsiella pneumoniae ST16: A Computational Exploration of Whole Genome Sequences for Beta-lactam Resistance and the Discovery of NMD-1 Resistance Gene Inhibitor

Faleti

Ibisanmi

2023

Preprint

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Antibiotic resistance is a growing concern in the field of healthcare and medicine. This research project involves an exploration of the whole genome sequences of Klebsiella pneumoniae ST16 from NCBI database with the aim of identifying resistance gene and potentially discovering its inhibitor. The study revealed the existence of several resistance genes to various classes of antibiotics, including macrolides, fluoroquinolones, aminoglycosides, sulphonamides, rifampicin, trimethoprim, and beta-lactams. Among these, blaTEM-1B, blaCTX-M-15, and blaNDM-1 were identified. Additionally, mutations were observed in the genes acrR, ompK36, and gyrA, along with changes in the corresponding amino acids, which are linked to resistance to different antibiotics. This analysis also identified the alleles present in each locus, with FIA having a novel allele, the molecular docking results indicate that Baicalein exhibited the highest docking scores of -7.7 respectively, indicating their strong binding affinity to the NDM-1 found to be associated with beta-lactams. The RMSD plot showed that both the Baicalein and Adapalene complexes of NDM-1 exhibited stable behavior over the 50 ns simulation period. However, the slightly higher Rg of the New Delhi Metallo-Beta-Lactamase 1-Adapalene complex indicates that this complex may be slightly more flexible than the New Delhi Metallo-Beta-Lactamase 1-Baicalein complex. In conclusion, the study provides valuable insights into the mechanisms of antibiotic resistance, particularly the role of the NDM-1 gene in beta-lactam resistance. Furthermore, the molecular docking analysis identified Baicalein, Taxifolin, and Ellagic acid as the top three bioactive compounds that exhibited strong binding affinity to the NDM-1 domain.

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Section: Discussionmentioning

confidence: 99%

Cracking the Code of Antibiotic Resistance OF Klebsiella pneumoniae ST16: A Computational Exploration of Whole Genome Sequences for Beta-lactam Resistance and the Discovery of NMD-1 Resistance Gene Inhibitor

Faleti

Ibisanmi

2023

Preprint

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“…Efflux pumps are specialized proteins located in the bacterial cell membrane that actively expel various substances, including antibiotics. 55 NorAn, specifically, is commonly found in Staphylococcus aureus and plays a defensive role by facilitating the removal of antibiotics from the cell, thereby reducing their efficacy. This efflux pump variant presents a challenging hurdle in the treatment of bacterial infections, as it diminishes the intracellular concentration of antibiotics and contributes to multidrug resistance.…”

Section: Mechanisms Of Amr Development In S Aureusmentioning

confidence: 99%

Methicillin Resistant Staphylococcus aureus: Molecular Mechanisms Underlying Drug Resistance Development and Novel Strategies to Combat

Abebe,

Birhanu

2023

IDR

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Antimicrobial resistance (AMR) represents a major threat to global health. Infection caused by Methicillin-resistant Staphylococcus aureus (MRSA) is one of the well-recognized global public health problem globally. In some regions, as many as 90% of S. aureus infections are reported to be MRSA, which cannot be treated with standard antibiotics. WHO reports indicated that MRSA is circulating in every province worldwide, significantly increasing the risk of death by 64% compared to drug-sensitive forms of the infection which is attributed to its antibiotic resistance. The emergence and spread of antibiotic-resistant MRSA strains have contributed to its increased prevalence in both healthcare and community settings. The resistance of S. aureus to methicillin is due to expression of penicillin-binding protein 2a (PBP2a), which renders it impervious to the action of β-lactam antibiotics including methicillin. The other is through the production of beta-lactamases. Although the treatment options for MRSA are limited, there are promising alternatives to antibiotics to combat the infections. Innovative therapeutic strategies with wide range of activity and modes of action are yet to be explored. The review highlights the global challenges posed by MRSA, elucidates the mechanisms underlying its resistance development, and explores mitigation strategies. Furthermore, it focuses on alternative therapies such as bacteriophages, immunotherapy, nanobiotics, and antimicrobial peptides, emphasizing their synergistic effects and efficacy against MRSA. By examining these alternative approaches, this review provides insights into the potential strategies for tackling MRSA infections and combatting the escalating threat of AMR. Ultimately, a multifaceted approach encompassing both conventional and novel interventions is imperative to mitigate the impact of MRSA and ensure a sustainable future for global healthcare.

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A Computational Exploration of Whole Genome Sequences of Klebsiella pneumoniae ST16 for Beta-lactam Resistance and the Discovery of NMD-1 Resistance Gene Inhibitor

Ibisanmi,

Olowosoke,

Ayeni

et al. 2023

Preprint

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Antibiotic resistance is a growing concern in healthcare and medicine. This research focuses on studying the sequences of Klebsiella pneumoniae ST16 from the NCBI database. The goal is to identify genes that cause resistance to antibiotics and potentially find substances that can inhibit them. The study discovered genes that contribute to resistance against types of antibiotics such as macrolides, fluoroquinolones, aminoglycosides, sulphonamides, rifampicin, trimethoprim, and beta-lactams. Notable genes identified include blaTEM 1B, blaCTX M 15, and blaNDM-1. Furthermore, changes were observed in the acrR, ompK36, and gyrA genes, along with alterations in the corresponding acids, which are associated with resistance. The analysis also examined the alleles at each locus and found that FIA had a new allele. Molecular docking results revealed that baicalein showed docking scores of -7.7 kcal/mol when binding with New Delhi Metallo 1 (NDM-1) related to beta-lactams. The RMSD plot demonstrated behavior for both Baicalein and Adapalene complexes of NDM-1 over a 50 ns simulation period. However, the higher Rg value for the NDM-1 Beta-Lactamase 1-Adapalene complex indicates it may have slightly more flexibility compared to the NDM-1 Beta-Lactamase 1-Baicalein complex. Summarily, the study offers information about how antibiotic resistance works in relation to the NDM-1 gene and its role in beta-lactam resistance based on analysis which reveals that beyond baicalein, other excellent bioactive (taxifolin, and ellagic acid) strongly bind to the NDM 1 domain and can be further investigated experimentally.

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The Linkage Between Antibiotic and Disinfectant Resistance

Cited by 3 publications

References 209 publications

Cracking the Code of Antibiotic Resistance OF Klebsiella pneumoniae ST16: A Computational Exploration of Whole Genome Sequences for Beta-lactam Resistance and the Discovery of NMD-1 Resistance Gene Inhibitor

Cracking the Code of Antibiotic Resistance OF Klebsiella pneumoniae ST16: A Computational Exploration of Whole Genome Sequences for Beta-lactam Resistance and the Discovery of NMD-1 Resistance Gene Inhibitor

Methicillin Resistant Staphylococcus aureus: Molecular Mechanisms Underlying Drug Resistance Development and Novel Strategies to Combat

A Computational Exploration of Whole Genome Sequences of Klebsiella pneumoniae ST16 for Beta-lactam Resistance and the Discovery of NMD-1 Resistance Gene Inhibitor

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