The linkage arrangement of four rabbit β-like globin genes

Lacy, Elizabeth; Hardison, Ross C.; Quon, Diana; Maniatis, Tom

doi:10.1016/0092-8674(79)90238-1

Cited by 130 publications

(43 citation statements)

References 46 publications

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“…An argument for the presence of both aA and as genes in the chicken genome would require that the chromosomal gene corresponding to the aA-amino acid sequence either be unlinked to the rest of the chicken a-globin genes or be so different in nucleotide sequence as to be unable to hybridize to aD-or astype probes. The latter possibility seems unlikely because there are long regions of close correspondence between aA-and asamino acid sequences (2,5), and the former possibility contradicts all other globin gene linkage groups (14,(22)(23)(24). The chicken globin gene linkage groups, in fact, seem to exhibit extremely close linkage properties, with adjacent genes rarely more than 3 kbp apart (10,15).…”

Section: Discussionmentioning

confidence: 98%

“…Restriction analyses were done by single and multiple digestions of phage DNA with commercial restriction enzymes followed by analysis on agarose gels and Southern (13) hybridization ofthe blots from these gels to various labeled nucleic acids (10,12). Subcloning ofDNA fragments isolated from these phage DNAs into pBR322 plasmid DNAs was performed as described (14)(15)(16) (18) were used. Singly end-labeled restriction enzyme fragments were isolated from ethidium bromide-stained agarose gels as described (19).…”

Section: Methodsmentioning

confidence: 99%

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Adult chicken alpha-globin genes alpha A and alpha D: no anemic shock alpha-globin exists in domestic chickens.

Dodgson¹,

McCune²,

Rusling³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

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Three a-type globin genes have been identified in the a-globin linkage group of chickens. No other a-type genes have been directly shown to be within 10 kilobase pairs of any of these three closely linked genes. These three genes have been conclusively identified by DNA sequence analysis. The gene at the 5' end of the linkage group is an embryonic a-type globin gene, ar or ir, and the central gene corresponds to the minor adult aglobin, aD. The 3'-terminal gene sequence corresponds to the sequence of cDNA clones previously described as "71", a presumed anemic shock-induced a-globin gene

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Adult chicken alpha-globin genes alpha A and alpha D: no anemic shock alpha-globin exists in domestic chickens.

Dodgson¹,

McCune²,

Rusling³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

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“…As shown in Fig. 6 and Table 1 related proteins is known in other systems as well e.g., the histone genes of the sea urchin (4), the chorion protein genes of wild silk moths (17) and Drosophila (33), and the globin genes of mammals (13,20,21). In the globin gene system, heterotypic linked genes are expressed at different stages and in different cells, whereas in all the other examples cited the linked genes appear to function at more or less the same time in the same differentiated cells.…”

Section: Discussionmentioning

confidence: 99%

Organization and expression of multiple actin genes in the sea urchin.

Scheller¹,

McAllister²,

Crain³

et al. 1981

Mol. Cell. Biol.

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A set of at least 11 actin genes has been isolated from genomic recombinant deoxyribonucleic acid libraries of the sea urchin Strongylocentrotus purpuratus. Most of the isolates derive from a library which represents the genome of a single animal. There are at least five distinct types of sea urchin actin gene, some of which are represented by multiple copies in the genome. The actin gene types are distinguished by nonhomologous flanking sequences and intervening sequences, though the protein coding sequences appear in most cases to be quite similar.Eight of the 11 genes isolated have been recovered in X recombinants that contain two actin genes, linked at 5-to 9-kilobase distances. Restriction map overlaps suggest that the genome contains an array of at least three of these genes spaced over about 30 kilobases of deoxyribonucleic acid. In the linkage patterns observed, actin genes of diverse types were linked to each other. In early embryos, actin messenger ribonucleic acid (RNA) transcripts of 1.8 and 2.2 kilobases were found, and the longer of these transcripts was more prevalent in the maternal RNA of the egg. From RNA gel blot experiments, we conclude that the two transcripts derive from different actin gene types. Different repetitive sequences were located to either side of most of the actin genes, and in most observed cases the repeat sequences which were adjacent to actin genes of a given type were similar. The repeat sequences flanking the actin genes belonged to families which were transcribed, but those repeats in the neighborhood of the actin genes which have been investigated were not themselves represented in the stable RNAs of eggs or early embryos.

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“…The rabbit ,B-like globin gene family is a set of structurally related genes that are differentially expressed during development (22,32). Gene 133 and 134 are expressed in embryonic life, gene 13 is expressed in fetal and adult life (22,50), and gene 4432 is an unexpressed pseudogene (33).…”

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confidence: 99%