The Limiting Role of Phosphorus in a Woodland Stream Ecosystem: Effects of P Enrichment on Leaf Decomposition and Primary Producers

Abstract

The limiting role of phosphorus on leaf decomposition and primary producers was investigated in a second—order woodland stream in Tennessee by experimentally enriching, for 95 d, adjacent reaches with an average of 60 and 450 mg PO4—P/L, respectively, over upstream control levels of °4 mg/L. Red oak (Quercus rubra) leaf packs in the enriched sections lost mass 24% faster than control packs (P < .05). Nitrogen content of the enriched packs increased 60% more, and P content increased 83% more than the respective increases in the control packs (P < .05). Differences in mass loss and N and P levels between the low and high enrichments were not significant (P > .05). Respiration rates of subsampled leaf discs were significantly higher than control rates only at the high level of enrichment. The increased respiration rates in the low and high enrichments accounted for 10 and 34% of the increased mass loss in the respective enriched sections, suggesting that the enrichment also produced increases in mechanical breakdown through faster microbial conditioning, increases in macroinvertebrate feeding, or both. Effects of the enrichment on aufwuchs initially consisted of increased chlorophyll a levels, followed by increased aufwuchs biomass levels. Dense growth of filamentous algae, including some Oscillatoria, which may be a nitrogen fixer, developed immediately downstream of P inputs. In addition, Nostoc, a known nitrogen—fixing blue—green alga, sampled after the enrichment, was significantly more abundant in the enriched sections than the control (P < .05). Densities of the snail, Goniobasis clavaeformis, a grazer—shredder sampled after the enrichment, also were significantly greater in the enriched reaches, suggesting that the lack of a sustained response of chlorophyll a to the enrichment may have been a result of increased grazing on algal biomass. These findings indicate that nutrient limitation of detrital processing is a significant factor in natural streams. The apparent increases in densities of benthic macroinvertebrates in the enriched sections, along with reported relationships between detrital food richness and macroinvertebrate growth and survivorship, suggest that nutrient limitation in streams also has ramifications on higher tropic levels.

Modifications of ecological trophic structures on chemical gradients in lotic ecosystems and their relations to stream ecosystem health

“…The high N:P mass ratios of 17 indicate phosphorus limitation in the ecosystem and low N:P ratios of B4 indicate nitrogen limitation (Forsberg & Ryding 1980;Dodds 2006). This reference indicates that our streams largely have severe phosphorus limitation (Elwood et al 1981), based on the N:P ratio calculations on the trophic components. When the N:P ratios were B100, the dominant taxa with 42.2% of the total was Zacco platypus, which was an OS in the trophic levels as well as TS in tolerance guilds.…”

Contrasting response of stream detritivores to long-term nutrient enrichment

“…In detritus-based stream ecosystems, nutrient enrichment is capable of stimulating microbial biomass and activity (e.g., Ramírez et al 2003;Stelzer et al 2003), invertebrate biomass and production (e.g., Pearson and Connolly 2000;Rosemond et al 2002;Cross 2004), and leaf litter decomposition (e.g., Elwood et al 1981;Gulis and Suberkropp 2003). During a sufficient time period, these factors may lead to a net loss of carbon from the system (Rosemond unpubl.…”

Nutrient Uptake and Mineralization during Leaf Decay in Streams – a Model Simulation

“…and fungal activity are correlated with water column nutrient concentration (e.g., ELWOOD et al, 1981;MEYER and JOHNSON, 1983;SUBERKROPP and CHAUVET, 1995;GESSNER et al, 2007). Second, the studies described above showing the correlation between nutrient uptake and leaf standing crop rely on decline in water column concentrations to quantify uptake and are thus clear evidence that microbes are using these dissolved nutrients.…”