2016
DOI: 10.1038/onc.2016.418
|View full text |Cite
|
Sign up to set email alerts
|

The LIM protein AJUBA promotes colorectal cancer cell survival through suppression of JAK1/STAT1/IFIT2 network

Abstract: The LIM protein AJUBA is a scaffold protein participating in the regulation of cell adhesion, mitosis, DNA damage, cell differentiation, proliferation, migration and gene transcription. However, its roles in tumorigenesis and progression are poorly defined. Here, we report that AJUBA is highly expressed in colorectal cancer (CRC) and promotes CRC cell growth in culture and in xenografted mice via an inhibition of apoptosis. AJUBA represses the expression of IFIT2 gene, an interferon-stimulated gene and a known… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

4
60
0

Year Published

2017
2017
2023
2023

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 54 publications
(64 citation statements)
references
References 33 publications
4
60
0
Order By: Relevance
“…335 AJUBA is a scaffold protein that participates in the regulation of cell adhesion, differentiation, proliferation, and migration and promotes the survival and proliferation of colorectal CSCs via the JAK1/STAT1 pathway. 336 Moreover, microRNAs are also involved in activating JAK/STAT signaling by inhibiting negative regulatory factors of JAK2/STAT3. For example, miR-500a-3p targets multiple negative regulators of the JAK2/STAT3 signaling pathway, such as SOCS2, SOCS4, and PTPN, in HCC stem cells, leading to constitutive activation of STAT3 signaling.…”
Section: Major Signaling Pathways In Cscsmentioning
confidence: 99%
“…335 AJUBA is a scaffold protein that participates in the regulation of cell adhesion, differentiation, proliferation, and migration and promotes the survival and proliferation of colorectal CSCs via the JAK1/STAT1 pathway. 336 Moreover, microRNAs are also involved in activating JAK/STAT signaling by inhibiting negative regulatory factors of JAK2/STAT3. For example, miR-500a-3p targets multiple negative regulators of the JAK2/STAT3 signaling pathway, such as SOCS2, SOCS4, and PTPN, in HCC stem cells, leading to constitutive activation of STAT3 signaling.…”
Section: Major Signaling Pathways In Cscsmentioning
confidence: 99%
“…The co-immunoprecipitation (co-IP), Western blotting, and glutathione S-transferase (GST) pull-down assays were described previously (Jia et al, 2017). In brief, cells were lysed 48 h of post-transfection in buffer containing 20 mM/L Tris-HCl (pH 8.0), 150 mM/L NaCl, 2.5 mM/L EDTA, 0.5% NP40, 0.1 mM/L phenylmethylsulfonyl fluoride (PMSF), and protease inhibitor cocktail.…”
Section: Western Blot Co-ip and Gst Pull-downmentioning
confidence: 99%
“…On the other hand, overexpression of LncRNA00364 fail to show any apparent effects on the expression of OAS1 and ISG15 (Figure 4A and Supplementary Figure 1C and 1D ), suggesting its specific effect on IFIT2. IFIT2 is well known as an important inducer of apoptosis and therefore serves as an important tumor suppressor [ 26 28 ]. To examine the physiological relevance of IFIT2 in HCC, we examined its expression in normal and HCC patients in several independent datasets available through the oncomine database.…”
Section: Resultsmentioning
confidence: 99%