The Light-Induced WD40-Repeat Transcription Factor DcTTG1 Regulates Anthocyanin Biosynthesis in Dendrobium candidum

Jia, Ning; Wang, Jingjing; Wang, Yajuan; Ye, Wei; Liu, Jiameng; Jiang, Jinlan; Sun, Jing; Yan, Peipei; Wang, Peiyu; Wang, Fengzhong; Fan, Bin

doi:10.3389/fpls.2021.633333

Cited by 16 publications

(15 citation statements)

References 45 publications

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“…We speculated that quercetin-3-Oglucoside, pelargonidin-3-O-sambubioside, cyanidin glycoside derivatives, or rutin glycoside derivatives of cyanidin determine the color of the D. officinale stems. This finding is consistent with previously reported results (Jia et al, 2021).…”

Section: Quantitative Metabolome Analyses Reveal Hl-induced Alteratio...supporting

confidence: 94%

High light triggers flavonoid and polysaccharide synthesis through DoHY5‐dependent signaling in Dendrobium officinale

Liu

et al. 2023

The Plant Journal

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SUMMARYDendrobium officinale is edible and has medicinal and ornamental functions. Polysaccharides and flavonoids, including anthocyanins, are important components of D. officinale that largely determine the nutritional quality and consumer appeal. There is a need to study the molecular mechanisms regulating anthocyanin and polysaccharide biosynthesis to enhance D. officinale quality and its market value. Here, we report that high light (HL) induced the accumulation of polysaccharides, particularly mannose, as well as anthocyanin accumulation, resulting in red stems. Metabolome and transcriptome analyses revealed that most of the flavonoids showed large changes in abundance, and flavonoid and polysaccharide biosynthesis was significantly activated under HL treatment. Interestingly, DoHY5 expression was also highly induced. Biochemical analyses demonstrated that DoHY5 directly binds to the promoters of DoF3H1 (involved in anthocyanin biosynthesis), DoGMPP2, and DoPMT28 (involved in polysaccharide biosynthesis) to activate their expression, thereby promoting anthocyanin and polysaccharide accumulation in D. officinale stems. DoHY5 silencing decreased flavonoid‐ and polysaccharide‐related gene expression and reduced anthocyanin and polysaccharide accumulation, whereas DoHY5 overexpression had the opposite effects. Notably, naturally occurring red‐stemmed D. officinale plants similarly have high levels of anthocyanin and polysaccharide accumulation and biosynthesis gene expression. Our results reveal a previously undiscovered role of DoHY5 in co‐regulating anthocyanin and polysaccharide biosynthesis under HL conditions, improving our understanding of the mechanisms regulating stem color and determining nutritional quality in D. officinale. Collectively, our results propose a robust and simple strategy for significantly increasing anthocyanin and polysaccharide levels and subsequently improving the nutritional quality of D. officinale.

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Section: Quantitative Metabolome Analyses Reveal Hl-induced Alteratio...supporting

confidence: 94%

High light triggers flavonoid and polysaccharide synthesis through DoHY5‐dependent signaling in Dendrobium officinale

Liu

et al. 2023

The Plant Journal

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“…WDR proteins are a very large family. It has been found that WDR proteins play a critical role in the regulation of anthocyanin biosynthesis in many plant species [ 40 , 48 , 49 , 50 , 51 , 52 ]. WDR physically interacts with MYB and bHLH to form a transcriptional complex, which participates the regulation of the flavonoid pigment pathway [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Construction of a High-Density Recombination Bin-Based Genetic Map Facilitates High-Resolution Mapping of a Major QTL Underlying Anthocyanin Pigmentation in Eggplant

Guan

Tang

et al. 2022

IJMS

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High-density genetic maps can significantly improve the resolution of QTL mapping. We constructed a high-density recombination bin-based genetic map of eggplant based on 200 F2 plants from an interspecific cross (Solanum melongena × S. incanum) using the whole genome resequencing strategy. The map was 2022.8 cM long, covering near 99% of the eggplant genome. The map contained 3776 bins, with 3644 (96.5%) being effective (position non-redundant) ones, giving a nominal average distance of 0.54 cM and an effective average distance of 0.56 cM between adjacent bins, respectively. Using this map and 172 F2:3 lines, a major QTL with pleiotropic effects on two anthocyanin pigmentation-related traits, leaf vein color (LVC) and fruit pericarp color (FPC), was steadily detected in a bin interval of 2.28 cM (or 1.68 Mb) on chromosome E10 in two cropping seasons, explaining ~65% and 55% of the phenotypic variation in LVC and FPC, respectively. Genome-wide association analysis in this population validated the QTL and demonstrated the correctness of mapping two bins of chromosome E02 onto E10. Bioinformatics analysis suggested that a WDR protein gene inside the bin interval with reliable effective variation between the two parents could be a possible candidate gene of the QTL.

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“…The WD40 repeat protein in Medicago truncatula is required for tissue-specific anthocyanin and proanthocyanidin biosynthesis but not for trichome development (Pang et al, 2009). Light promoted anthocyanin accumulation in dendrobium seedlings through the upregulation of the WD40 repeat transcription factor DcTTG1, which induced the expression of anthocyanin synthesis-related genes (Jia et al, 2021). In Arabidopsis, the WD40 repeat protein TTG1 forms a complex with bHLH (GL3, EGL3, or TT8) and R2R3-MYB (PAP1, PAP2, MYB113, and MYB114) TFs regulated anthocyanin synthesis (Zhang et al, 2003;Gonzalez et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The long noncoding RNA LINC15957 regulates anthocyanin accumulation in radish

Tan

Luo²,

Lu³

et al. 2023

Front. Plant Sci.

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Radish (Raphanus sativus L.) is an important root vegetable crop belonging to the Brassicaceae family. Anthocyanin rich radish varieties are popular among consumers because of their bright color and high nutritional value. However, the underlying molecular mechanism responsible for skin and flesh induce anthocyanin biosynthesis in transient overexpression, gene silencing and transcriptome sequencing were used to verify its function in radish anthocyanin accumulation, radish remains unclear. Here, we identified a long noncoding RNA LINC15957, overexpression of LINC15957 was significantly increased anthocyanin accumulation in radish leaves, and the expression levels of structural genes related to anthocyanin biosynthesis were also significantly increased. Anthocyanin accumulation and expression levels of anthocyanin biosynthesis genes were significantly reduced in silenced LINC15957 flesh when compared with control. By the transcriptome sequencing of the overexpressed LINC15957 plants and the control, 5,772 differentially expressed genes were identified. A total of 3,849 differentially expressed transcription factors were identified, of which MYB, bHLH, WD40, bZIP, ERF, WRKY and MATE were detected and differentially expressed in the overexpressed LINC15957 plants. KEGG enrichment analysis revealed the genes were significant enriched in tyrosine, L-Phenylalanine, tryptophan, phenylpropanol, and flavonoid biosynthesis. RT-qPCR analysis showed that 8 differentially expressed genes (DEGs) were differentially expressed in LINC15957-overexpressed plants. These results suggested that LINC15957 involved in regulate anthocyanin accumulation and provide abundant data to investigate the genes regulate anthocyanin biosynthesis in radish.

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The Light-Induced WD40-Repeat Transcription Factor DcTTG1 Regulates Anthocyanin Biosynthesis in Dendrobium candidum

Cited by 16 publications

References 45 publications

High light triggers flavonoid and polysaccharide synthesis through DoHY5‐dependent signaling in Dendrobium officinale

High light triggers flavonoid and polysaccharide synthesis through DoHY5‐dependent signaling in Dendrobium officinale

Construction of a High-Density Recombination Bin-Based Genetic Map Facilitates High-Resolution Mapping of a Major QTL Underlying Anthocyanin Pigmentation in Eggplant

The long noncoding RNA LINC15957 regulates anthocyanin accumulation in radish

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