The Light-Harvesting Chlorophyll a/b Binding Proteins Lhcb1 and Lhcb2 Play Complementary Roles during State Transitions in Arabidopsis

Pietrzykowska, Malgorzata; Suorsa, Marjaana; Semchonok, Dmitry A.; Tikkanen, Mikko; Boekema, Egbert J.; Aro, Eva‐Mari; Jansson, Stefan

doi:10.1105/tpc.114.127373

Cited by 254 publications

(231 citation statements)

References 69 publications

(95 reference statements)

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“…This was previously shown in an analysis of antisense lines deficient in Lhcb1 or Lhcb2 (Pietrzykowska et al, 2014). The lack of cross reactivity was further confirmed using recombinant proteins expressed in Escherichia coli (Supplemental Fig.…”

Section: An Improved Phos-tag Page Protocol Allows a Quantitative Meamentioning

confidence: 74%

“…Lhcb1 could largely replace Lhcb2 in the lines lacking Lhcb2. However these Lhcb2 knock-down mutants could not assemble the PSI-LHCII supercomplex or undergo state transitions (Pietrzykowska et al, 2014). Using phosphospecific antisera, it was also observed that PSI-LHCII is enriched in P-Lhcb2 but lacks P-Lhcb1 (Leoni et al, 2013).…”

Section: Antenna Phosphorylation In the Psi-lhcii State Transition Sumentioning

confidence: 83%

“…4), even though the presence of Lhcb1 appears to be crucial, since in knock-down lines lacking this isoform the supercomplex does not form (Pietrzykowska et al, 2014). The lack of Lhcb1 phosphorylation in PSI-LHCII could seem somewhat paradoxical, since STN7-dependent phosphorylation of both Lhcb1 and Lhcb2 is observed in conditions that favor a transition from state 1 to state 2 ( Fig.…”

Section: Antenna Phosphorylation In the Psi-lhcii State Transition Sumentioning

confidence: 92%

“…In the LHCII trimers of these supercomplexes, different classes of Lhcb subunits are distributed differently, suggesting a specific role in light acclimation for each of them (Damkjaer et al, 2009;Pietrzykowska et al, 2014). In the stably bound S trimer, Lhcb1 and Lhcb2 are more abundant, whereas the moderately bound M trimer contains mostly Lhcb1 and Lhcb3 (Galka et al, 2012).…”

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confidence: 99%

“…The differential association of photosystems, LHCII, and other components of the thylakoid membrane gives rise to a set of supercomplexes that are central in ensuring photosynthetic efficiency and a rapid response to environmental cues (Caffarri et al, 2009;Duffy et al, 2013;Pietrzykowska et al, 2014;Fristedt et al, 2015). Fine tuning the dynamic assembly of these supercomplexes involves the association of antennae containing specific sets of Lhcb proteins.…”

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confidence: 99%

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Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions

et al. 2015

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ORCID IDs: 0000-0003-0587-7621 (P.L.); 0000-0002-6155-9153 (D.D.).Light-harvesting complex II (LHCII) is a crucial component of the photosynthetic machinery, with central roles in light capture and acclimation to changing light. The association of an LHCII trimer with PSI in the PSI-LHCII supercomplex is strictly dependent on LHCII phosphorylation mediated by the kinase STATE TRANSITION7, and is directly related to the light acclimation process called state transitions. In Arabidopsis (Arabidopsis thaliana), the LHCII trimers contain isoforms that belong to three classes: Lhcb1, Lhcb2, and Lhcb3. Only Lhcb1 and Lhcb2 can be phosphorylated in the N-terminal region. Here, we present an improved Phos-tag-based method to determine the absolute extent of phosphorylation of Lhcb1 and Lhcb2. Both classes show very similar phosphorylation kinetics during state transition. Nevertheless, only Lhcb2 is extensively phosphorylated (.98%) in PSI-LHCII, whereas phosphorylated Lhcb1 is largely excluded from this supercomplex. Both isoforms are phosphorylated to different extents in other photosystem supercomplexes and in different domains of the thylakoid membranes. The data imply that, despite their high sequence similarity, differential phosphorylation of Lhcb1 and Lhcb2 plays contrasting roles in light acclimation of photosynthesis.

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Section: An Improved Phos-tag Page Protocol Allows a Quantitative Meamentioning

confidence: 74%

Section: Antenna Phosphorylation In the Psi-lhcii State Transition Sumentioning

confidence: 83%

Section: Antenna Phosphorylation In the Psi-lhcii State Transition Sumentioning

confidence: 92%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions

et al. 2015

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Photosynthetic Regulatory Mechanisms for Efficiency and Prevention of Photo‐Oxidative Stress

Roach

Krieger‐Liszkay

2019

Annual Plant Reviews Online

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Regulatory mechanisms of photosynthesis affecting light capture, the efficiency of electron transport, and the distribution between linear and alternative electron transport routes are needed to balance energy input with metabolic demands. Rapid fluctuations in light intensity lead transiently to absorption of excess light energy, requiring immediate regulation, whereas adverse environmental conditions over longer‐term require acclimation responses. In either case, excitation of photosynthetic pigments beyond metabolic demand and unpoised electron transfer reactions can lead to the generation of reactive oxygen species (ROS). On the one hand, ROS damage the photosynthetic machinery and other macro‐molecules, such as thylakoid membranes, contributing to photo‐oxidative stress. On the other hand, ROS are needed for acclimation responses since they affect the expression level of responsive genes, as well as exert direct and indirect effects on redox‐regulated processes in the chloroplast. In this article, we introduce the photosynthetic complexes, step‐by‐step and focus on the regulatory mechanisms required to enable photosynthetic efficiency and control levels of ROS production. Finally, we shortly describe the roles of chloroplast‐derived ROS in the short‐term modulation of photosynthesis and the longer‐term acclimation to light stress. Differences in regulatory mechanisms amongst the diversity of Viridiplantae (i.e. green algae to plants) are described.

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Comparative proteomics of thylakoids from Arabidopsis grown in laboratory and field conditions

et al. 2021

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Compared to controlled laboratory conditions, plant growth in the field is rarely optimal since it is frequently challenged by large fluctuations in light and temperature which lower the efficiency of photosynthesis and lead to photo‐oxidative stress. Plants grown under natural conditions therefore place an increased onus on the regulatory mechanisms that protect and repair the delicate photosynthetic machinery. Yet, the exact changes in thylakoid proteome composition which allow plants to acclimate to the natural environment remain largely unexplored. Here, we use quantitative label‐free proteomics to demonstrate that field‐grown Arabidopsis plants incorporate aspects of both the low and high light acclimation strategies previously observed in laboratory‐grown plants. Field plants showed increases in the relative abundance of ATP synthase, cytochrome b6f, ferredoxin‐NADP+ reductases (FNR1 and FNR2) and their membrane tethers TIC62 and TROL, thylakoid architecture proteins CURT1A, CURT1B, RIQ1, and RIQ2, the minor monomeric antenna complex CP29.3, rapidly‐relaxing non‐photochemical quenching (qE)‐related proteins PSBS and VDE, the photosystem II (PSII) repair machinery and the cyclic electron transfer complexes NDH, PGRL1B, and PGR5, in addition to decreases in the amounts of LHCII trimers composed of LHCB1.1, LHCB1.2, LHCB1.4, and LHCB2 proteins and CP29.2, all features typical of a laboratory high light acclimation response. Conversely, field plants also showed increases in the abundance of light harvesting proteins LHCB1.3 and CP29.1, zeaxanthin epoxidase (ZEP) and the slowly‐relaxing non‐photochemical quenching (qI)‐related protein LCNP, changes previously associated with a laboratory low light acclimation response. Field plants also showed distinct changes to the proteome including the appearance of stress‐related proteins ELIP1 and ELIP2 and changes to proteins that are largely invariant under laboratory conditions such as state transition related proteins STN7 and TAP38. We discuss the significance of these alterations in the thylakoid proteome considering the unique set of challenges faced by plants growing under natural conditions.

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The Light-Harvesting Chlorophyll a/b Binding Proteins Lhcb1 and Lhcb2 Play Complementary Roles during State Transitions in Arabidopsis

Cited by 254 publications

References 69 publications

Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions

Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions

Photosynthetic Regulatory Mechanisms for Efficiency and Prevention of Photo‐Oxidative Stress

Comparative proteomics of thylakoids from Arabidopsis grown in laboratory and field conditions

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