The Light-Addressable Potentiometric Sensor: Principles and Biological Applications

Owicki, John C.; Bousse, L.; Hafeman, Dean G.; Kirk, Gregory; Olson, John D.; Wada, H G; Parce, J W

doi:10.1146/annurev.bb.23.060194.000511

Cited by 244 publications

(109 citation statements)

References 34 publications

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“…Ion-sensitive field-effect transistors (ISFETs) and relatives (EnFETs, BioFETs, etc.) are the canonical examples [6], but similar mechanisms operate in semiconducting nanowires [7], semiconducting carbon nanotubes [8], electrolyte-insulator-semiconductor structures [9][10][11][12], suspended gate thin film transistors [13], and light-addressable potentiometric sensors [14,15]. These field-effect sensors rely on the interaction of external charges with carriers in a nearby semiconductor and thus exhibit enhanced sensitivity at low ionic strength where counterion shielding is reduced; this is explained in a recent review [16] and evidenced by the low salt concentrations often used (e.g., [7,10]).…”

Section: Introductionmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research.

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Section: Introductionmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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“…The metabolic activity of cells can be monitored by measuring the acidification rate in the extracellular environment; therefore, changes in the biological function and the response to ligand binding to surface receptors, and the impact of chemicals and drugs on cell physiology can be studied by measuring the decrease in pH that occurs as acidic metabolites build up in the extracellular space (Lorenzelli et al, 2003;Parak et al, 2000;Thedinga et al, 2007). volumes using a Light Addressable Potentiometric Sensor to determine acidification rates in order to assess the metabolic activity of cells during pharma-or toxicological studies (Eklund et al, 2003;Hafner, 2000;Owicki et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

On-chip acidification rate measurements from single cardiac cells confined in sub-nanoliter volumes

Ges

Dzhura

Baudenbacher

2008

Biomed Microdevices

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The metabolic activity of cells can be monitored by measuring the pH in the extracellular environment. Microfabrication and microfluidic technologies allow the sensor size and the extracellular volumes to be comparable to single cells. A glass substrate with thin film pH sensitive IrO x electrodes was sealed to a replica-molded polydimethylsiloxane (PDMS) microfluidic network with integrated valves. The device, termed NanoPhysiometer, allows the trapping of single cardiac myocytes and the measurement of the pH in a detection volume of 0.36 nL. For wild-type (WT) single cardiac myocytes an acidification rate of 6.45 ± 0.38 mpH/min was measured in comparison to 19.5 ± 0.38 mpH/min for very long chain Acyl-CoA dehydrogenase (VLCAD) deficient mice in 0.8 mM of Ca 2+ . VLCAD deficiency is a fatty acid oxidation disease leading to cardiomyopathy and arrhythmias. The acidification rate increased to 11.96 ± 1.33 mpH/min for WT and to 32.0 ± 4.64 mpH/min for VLCAD −/− in 1.8 mM of Ca 2+ . The NanoPhysiometer concept can be extended to study ischemia/reperfusion injury or disorders of other biological systems to identify strategies for treatment and possible pharmacological targets.

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“…The Cytosensor, based on the light-addressable potentiometric sensor technology (LAPS), is one of the oldest commercial cellbased sensor systems [32,33]. Research groups worldwide have made attempts to improve the LAPS based sensors system technology [34][35][36][37].…”

Section: Molecular Devices Cytosensor Microphysiometermentioning

confidence: 99%

A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment

Bohrn

Mucha

Werner

et al. 2013

Sensors and Actuators B: Chemical

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a b s t r a c tThe toxicity of chromium ions was investigated using mammalian cell cultures on impedance sensors as well as physiological in vitro sensor systems. The performance of commercially available systems like the 2500 Analyzing System (Bionas), xCELLigence (Roche) and Cytosensor Microphysiometer (Molecular Devices) was compared with a novel CMOS-based impedance-to-frequency converter device. Cell-based sensor systems are shown to be powerful tools to detect Cr(VI) pollutions within several hours in the range of multinational drinking water regulations. The ability to distinguish between toxic Cr(VI) and non-toxic Cr(III) species is one advantage of these integral sensor systems. Impedance only devices are not sufficient for the fast detection of toxic chromium species as rapid cellular changes occur only in the respiration system and the cell physiology.

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The Light-Addressable Potentiometric Sensor: Principles and Biological Applications

Cited by 244 publications

References 34 publications

Label‐Free Impedance Biosensors: Opportunities and Challenges

Label‐Free Impedance Biosensors: Opportunities and Challenges

On-chip acidification rate measurements from single cardiac cells confined in sub-nanoliter volumes

A critical comparison of cell-based sensor systems for the detection of Cr(VI) in aquatic environment

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