The ligation amplification reaction (LAR)—Amplification of specific DNA sequences using sequential rounds of template-dependent ligation

Wu, Dong; Rb, Wallace

doi:10.1016/0888-7543(89)90280-2

Cited by 251 publications

(108 citation statements)

References 18 publications

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“…97 Automated fluorescence-based DNA sequence analysis can generate 600 to 800 nucleotides of sequence data per reaction, and in some sense is parallel in that multiple gel electrophoresis lanes can be analyzed at the same time, as is true for SSCP, DGGE and some of the other methods. Allele-specific mutation/polymorphism detection methods include direct DNA sequence analysis, 98 reverse dot-blot with allele-specific oligonucleotide hybridization, 99 allele-specific PCR amplification, 100 oligonucleotide ligation amplification (OLA), 101 artificial introduction of restriction sites, 102 ligase chain reaction 103 and DNA minisequence analysis. [104][105][106] PCR-OLA has been applied to DNA diagnostics, 107 genetic mapping using biallelic markers 108 and YAC library screening.…”

Section: Non Microarray-based Parallel Mutation Detection Methodsmentioning

confidence: 99%

Parallel molecular genetic analysis

et al. 1998

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We describe recent progress in parallel molecular genetic analyses using DNA microarrays, gel-based systems, and capillary electrophoresis and utilization of these approaches in a variety of molecular biology assays. These applications include use of polymorphic markers for mapping of genes and disease-associated loci and carrier detection for genetic diseases. Application of these technologies in molecular diagnostics as well as fluorescent technologies in DNA analysis using immobilized oligonucleotide arrays on silicon or glass microchips are discussed. The array-based assays include sequencing by hybridization, cDNA expression profiling, comparative genome hybridization and genetic linkage analysis. Developments in non microarraybased, parallel analyses of mutations and gene expression profiles are reviewed. The promise of and recent progress in capillary array electrophoresis for parallel DNA sequence analysis and genotyping is summarized. Finally, a framework for decision making in selecting available technology options for specific molecular genetic analyses is presented.

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Section: Non Microarray-based Parallel Mutation Detection Methodsmentioning

confidence: 99%

Parallel molecular genetic analysis

et al. 1998

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“…However, little evidence was provided of differences in the rate of ligation between DNA containing and not containing a mismatch at the ligation site. Recently, conditions have been found that allow only minimal ligation when a mismatch occurs either side of the point of ligation, paving the way for a simple method of assaying for the presence of a particular mutation (Landegren et al, 1988a;Alves & Carr, 1988;Wu & Wallace, 1989b). The system used has been a continuous strand of DNA to which two adjacent oligonucleotides are hybridized with 3' and 5' bases of each abutting, so that ifjoined the region would be complementary to the continuous strand (Fig.…”

Section: Mutation Detection Methods Where the Mutation Position Is Knmentioning

confidence: 99%

Detection of single base changes in nucleic acids

Cotton

1989

Biochemical Journal

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“…For reviews, see references 94 and 105. Besides the molecular techniques described above, a number of other amplification techniques are used in the clinical microbiology laboratory, although not to detect antibiotic re- sistance determinants. These techniques include the DNA amplification techniques of strand displacement amplification (377,378) and ligase chain reaction (16,19,25,398,405) and the RNA amplification techniques of Q␤ replication (156) and self-sustained sequence replication or nucleic acid-based sequence amplification (55,148). This latter method can be modified to amplify DNA (99).…”

Section: Fig 3 the Scorpions Primer Is An Extension Of Molecular Bementioning

confidence: 99%