THE LIFE SPAN OF IgA PLASMA CELLS FROM THE MOUSE INTESTINE

Mattioli, Carlos A.; Tomasi, Thomas B.

doi:10.1084/jem.138.2.452

Cited by 82 publications

(30 citation statements)

References 8 publications

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“…BrdU-incorporated IgA-PCs reduced rapidly after the removal of BrdU, and most of the labeled cells disappeared on a chase period of 30 days. This is in reasonable accordance with previously reported cell kinetics study on LP lymphocytes (36). Essentially no difference was observed between the two different mouse strains, indicating that the high frequency of IgA-PCs in HIGA mice is not due to enhanced proliferation or prolonged survival of IgA ϩ cells in LP.…”

Section: Cell Cycle Analysis Of Iga-pcs In Lpsupporting

confidence: 81%

Increased Frequency of Surface IgA-Positive Plasma Cells in the Intestinal Lamina Propria and Decreased IgA Excretion in Hyper IgA (HIGA) Mice, a Murine Model of IgA Nephropathy with Hyperserum IgA

Kamata

Nogaki

Fagarasan

et al. 2000

The Journal of Immunology

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Because abnormalities of mucosal immunity have been suggested in human IgA nephropathy, we examined the involvement of mucosal immunity in IgA deposition to the kidney in hyper IgA (HIGA) mice, which was established as a mouse model for human IgA nephropathy with hyperserum IgA. The number of surface IgA+B220− lymphocytes in the intestinal lamina propria (LP) of HIGA mice increased 2.7-fold at 30 wk of age as compared with those at 10 wk of age, whereas normal mice did not show such increase. The surface IgA+B220− LP lymphocytes spontaneously secreted IgA in culture. Morphological studies showed that the surface IgA+B220− lymphocytes of murine intestinal LP are identical with plasma cells (PCs). About 20% of IgA+B220− PC in LP expressed both Mac-1 and CD19, suggesting that they may derive from peritoneal B-1 cells. Cell cycle study on intestinal IgA-PCs using bromodeoxyuridine revealed no difference between HIGA mice and normal mice, suggesting that the high frequency of IgA-producing PCs in HIGA mice is not due to enhanced proliferation or prolonged survival of IgA-producing PCs in LP. In addition, IgA secretion into the gut lumen of HIGA mice decreased drastically (to one forth) with aging. These data suggest that the increased number of intestinal IgA-producing PCs and the down-regulation of IgA excretion into the intestinal lumen might synergistically contribute to the hyperserum IgA in HIGA mice and resultant IgA deposition to the kidney.

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Section: Cell Cycle Analysis Of Iga-pcs In Lpsupporting

confidence: 81%

Increased Frequency of Surface IgA-Positive Plasma Cells in the Intestinal Lamina Propria and Decreased IgA Excretion in Hyper IgA (HIGA) Mice, a Murine Model of IgA Nephropathy with Hyperserum IgA

Kamata

Nogaki

Fagarasan

et al. 2000

The Journal of Immunology

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“…Alternatively, this may indicate that a proportion of lamina propria plasma cells is short-lived. This would be in keeping with data from in vivo mouse studies showing that small intestine plasma cells belong predominantly to a short-lived population (9,26).…”

Section: Discussionsupporting

confidence: 60%

“…In the small intestine of mice, the average half-life of lamina propria plasma cells was estimated to be 5 d and a maximum life span of the order 6 to 8 wk (26). In apparent contrast to these observations, evidence for a long-lived anticommensal IgA response was observed by reversible colonization of germ-free mice, suggesting the existence of long-lived IgA plasma cells in the gut (27).…”

contrasting

confidence: 42%

Long-Lived Plasma Cells from Human Small Intestine Biopsies Secrete Immunoglobulins for Many Weeks In Vitro

Mesin

Niro

Thompson

et al. 2011

The Journal of Immunology

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To understand the biology of Ab-secreting cells in the human small intestine, we examined Ab production of intestinal biopsies kept in culture. We found sustained IgA and IgM secretion as well as viable IgA- or IgM-secreting cells after >4 wk of culture. The Ab-secreting cells were nonproliferating and expressing CD27 and CD138, thus having a typical plasma cell phenotype. Culturing of biopsies without tissue disruption gave the highest Ab production and plasma cell survival suggesting that the environment regulates plasma cell longevity. Cytokine profiling of the biopsy cultures demonstrated a sustained presence of IL-6 and APRIL. Blocking of the activity of endogenous APRIL and IL-6 with BCMA–Fc and anti-human IL-6 Ab demonstrated that both these factors were essential for plasma cell survival and Ab secretion in the biopsy cultures. This study demonstrates that the human small intestine harbors a population of nonproliferating plasma cells that are instructed by the microenvironment for prolonged survival and Ab secretion.

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“…These studies imply a steady turnover of intestinal plasma cells. The observations in mice by Mattioli and Tomasi [5] suggest a relatively short life-span for intestinal plasma cells and, as discussed by those workers, the half-life of 4.7 days may be an overestimate. Finally, the initial decline in plasma cell numbers in the first 4 days after thoracic duct cannulation in the present experiment is consistent with the turnover of the cells.…”

Section: Discussionmentioning

confidence: 91%

IgA‐containing plasma cells in the lamina propria of the gut: failure of a thoracic duct fistula to deplete the numbers in rat small intestine

Mayrhofer

Fisher

1979

Eur J Immunol

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The quantitative importance of the traffic of large lymphocytes through the thoracic duct to the maintenance of the IgA‐containing plasma cell population of the lamina propria of the gut was examined by draining the thoracic duct for periods of time up to 10 days. Contrary to the expectation that IgA‐secreting plasma cell numbers in the small intestine would decline exponentially with a half‐time of approximately 5 days, the numbers of cells remained relatively constant after an initial decline in the first 4 days of lymphatic diversion. Results of in vivo pulse labeling with tritiated thymidine suggest that plasma cell numers are maintained by cell proliferation within the intestinal lamina propria, and that this is a compensatory mechanism brought into play by removal of the normal supply of precursors from the thoracic duct. The compensatory local recruitment of dividing plasma cell precursors principally involves those committed to IgA synthesis. It is suggested that thoracic duct lymph diversion calls into play a local mechanism to make up the deficit of plasma cell precursors lost in the lymph. This may be an accentuation of normal regulation of IgA synthesis in the lamina propria, and suggests that the homing of thoracic duct large lymphocytes is not the only source of renewal of gut plasma cells.

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THE LIFE SPAN OF IgA PLASMA CELLS FROM THE MOUSE INTESTINE

Cited by 82 publications

References 8 publications

Increased Frequency of Surface IgA-Positive Plasma Cells in the Intestinal Lamina Propria and Decreased IgA Excretion in Hyper IgA (HIGA) Mice, a Murine Model of IgA Nephropathy with Hyperserum IgA

Increased Frequency of Surface IgA-Positive Plasma Cells in the Intestinal Lamina Propria and Decreased IgA Excretion in Hyper IgA (HIGA) Mice, a Murine Model of IgA Nephropathy with Hyperserum IgA

Long-Lived Plasma Cells from Human Small Intestine Biopsies Secrete Immunoglobulins for Many Weeks In Vitro

IgA‐containing plasma cells in the lamina propria of the gut: failure of a thoracic duct fistula to deplete the numbers in rat small intestine

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