Abstract:Pygidiopsis macrostomum Travassos, 1928, originally described based on a single specimen from Rattus norvegicus (Erxleben) in Brazil, has also been reported from a piscivorous bat, Noctilio leporinus mastivus Ribeiro, in Cuba (Odening 1969, Zdzitowiecki & Rutkowska 1980. We have previously redescribed this species from the holotype and adults obtained from hamsters Mesocricetus auratus Waterhouse experimentally infected with metacercariae from naturally infected guppies Poecilia vivipara Bloch and Schneider ta… Show more
“…The Syrian hamster ( Mesocricetus auratus Waterhouse, 1814) was used successfully as an experimental definitive host (Simões, Barbosa & Santos ; Simões et al . ).…”
Section: Introductionmentioning
confidence: 97%
“…pindoramensis, but no genetic or cytochemical data have been reported for either parasite species (Simões et al . , , ).…”
Section: Introductionmentioning
confidence: 99%
“…In Brazil, the common rat Rattus norvegicus (Erxleben, 1777) has been reported as a natural definitive host, as has a piscivorous bat, Noctilio leporinus mastivus True, 1884, from Cuba (Travassos 1928;Odening 1969;Zdzitowiecki & Rutkowska 1980). The Syrian hamster (Mesocricetus auratus Waterhouse, 1814) was used successfully as an experimental definitive host (Simões, Barbosa & Santos 2005;Simões et al 2009).…”
Section: Introductionmentioning
confidence: 99%
“…Previous studies have described the morphology and life cycle of A. (P.) pindoramensis, but no genetic or cytochemical data have been reported for either parasite species (Simões et al 2005(Simões et al , 2006(Simões et al , 2009.…”
Pygidiopsis macrostomum and Ascocotyle (Phagicola) pindoramensis (Digenea: Heterophyidae) parasitize guppies as intermediate hosts and, respectively, fish‐eating mammals or birds as definitive hosts. Heterophyids have zoonotic potential, and molecular studies associated with morphological and ecological aspects have helped to clarify their taxonomy and phylogeny. Poecilia vivipara naturally parasitized by metacercariae of both species (100% prevalence) exhibit no external signs of parasitism. In this work, four new sequences of P. macrostomum (18S rDNA, 28S rDNA and ITS2 rDNA) and one new sequence of A. (P.) pindoramensis (mtDNA cox‐1) are presented. Phylogeny reconstructions linked P. macrostomum to other heterophyids, but the separation of the Heterophyidae and Opisthorchiidae remains unclear. Additionally, we used indirect immunocytochemistry and the phalloidin‐fluorescence techniques allied with confocal laser scanning microscopy to describe muscular and neuronal structures of P. macrostomum. A complex arrangement of muscular fibres is associated with the tegument, suckers, gut and reproductive system. Radial fibres around the ventral sucker are thick, branched and extend to the body wall. High‐resolution confocal imaging revealed a typical digenean muscular arrangement and important heterophyid morphological traits. These data will support future control measures to reduce the parasitism in guppies reared in fish farming systems, especially for aquarium and experimental purposes.
“…The Syrian hamster ( Mesocricetus auratus Waterhouse, 1814) was used successfully as an experimental definitive host (Simões, Barbosa & Santos ; Simões et al . ).…”
Section: Introductionmentioning
confidence: 97%
“…pindoramensis, but no genetic or cytochemical data have been reported for either parasite species (Simões et al . , , ).…”
Section: Introductionmentioning
confidence: 99%
“…In Brazil, the common rat Rattus norvegicus (Erxleben, 1777) has been reported as a natural definitive host, as has a piscivorous bat, Noctilio leporinus mastivus True, 1884, from Cuba (Travassos 1928;Odening 1969;Zdzitowiecki & Rutkowska 1980). The Syrian hamster (Mesocricetus auratus Waterhouse, 1814) was used successfully as an experimental definitive host (Simões, Barbosa & Santos 2005;Simões et al 2009).…”
Section: Introductionmentioning
confidence: 99%
“…Previous studies have described the morphology and life cycle of A. (P.) pindoramensis, but no genetic or cytochemical data have been reported for either parasite species (Simões et al 2005(Simões et al , 2006(Simões et al , 2009.…”
Pygidiopsis macrostomum and Ascocotyle (Phagicola) pindoramensis (Digenea: Heterophyidae) parasitize guppies as intermediate hosts and, respectively, fish‐eating mammals or birds as definitive hosts. Heterophyids have zoonotic potential, and molecular studies associated with morphological and ecological aspects have helped to clarify their taxonomy and phylogeny. Poecilia vivipara naturally parasitized by metacercariae of both species (100% prevalence) exhibit no external signs of parasitism. In this work, four new sequences of P. macrostomum (18S rDNA, 28S rDNA and ITS2 rDNA) and one new sequence of A. (P.) pindoramensis (mtDNA cox‐1) are presented. Phylogeny reconstructions linked P. macrostomum to other heterophyids, but the separation of the Heterophyidae and Opisthorchiidae remains unclear. Additionally, we used indirect immunocytochemistry and the phalloidin‐fluorescence techniques allied with confocal laser scanning microscopy to describe muscular and neuronal structures of P. macrostomum. A complex arrangement of muscular fibres is associated with the tegument, suckers, gut and reproductive system. Radial fibres around the ventral sucker are thick, branched and extend to the body wall. High‐resolution confocal imaging revealed a typical digenean muscular arrangement and important heterophyid morphological traits. These data will support future control measures to reduce the parasitism in guppies reared in fish farming systems, especially for aquarium and experimental purposes.
“…The group is characterized by small species that live in marine, brackish, and freshwater environments, and whose shells generally are less than 10 mm in length (Biese 1944(Biese , 1947Hershler and Thompson 1992). The species have a wide range of ecological tolerances and reproductive strategies Marcus 1963, 1965;Cazzaniga 1982a;Martín 2002;Neves et al 2010;Collado and Méndez 2011), and some of them serve as intermediate hosts in the life cycles of digenean trematodes (Etchegoin and Martorelli 1997;Simões et al 2008Simões et al , 2009Simões et al , 2010Alda et al 2010;Merlo and Etchegoin 2011). Kroll et al (2012) studied the phylogenetic relationships of species mainly distributed in the Altiplano region and its major internal Lake Titicaca, and this is the only phylogenetic study performed in the genus Heleobia.…”
Background: The Chilean Altiplano and the Atacama Desert in northern Chile harbor isolated hydrological systems or oases where it is possible to find minute aquatic snails of the genus Heleobia whose taxonomy is uncertain and where many populations remain unknown. Here, we obtained samples from 30 localities distributed in the region and used molecular (12S and 16S mitochondrial genes) and morphological (penis) characters to investigate diversity of this poorly known fauna. Results: Molecular phylogenetic analysis consistently recovered five clades, one of which constitutes a cryptic species previously assigned to a species recognized in the area. Four other clades contained sequences of one nominal species consistent with its type locality and at least two additional candidate species, which were corroborated by a particular penis morphology. Furthermore, some morphological differences in penis morphology were observed in two Altiplano populations not resolved by the DNA sequences, providing support for two additional candidate species in the genus. A molecular clock analysis allowed tracing the origin of lineages back to the Early Pleistocene.
Pygidiopsis cambodiensis n. sp. is described based on adult flukes recovered from Syrian golden hamsters experimentally infected with metacercariae from mullets (Liza macrolepis) purchased at a local fish market in Phnom Penh, Cambodia. The specimens were examined by light and scanning electron microscopy. Among the 13 species so far assigned to Pygidiopsis, the new species belongs to the summa-type (including Pygidiopsis pelecani, Pygidiopsis phalacrocoracis, Pygidiopsis piclaumoreli, Pygidiopsis plana, and Pygidiopsis summa) which lack circumoral spines and have vitelline follicles extending posteriorly from the level of the ovary some distance into the post-testicular space and the uterus not exceeding the acetabulum anteriorly. The new species differs from the other five species of the summa-type particularly in the morphology of the ventrogenital complex, including the genital sac, gonotyl, and gonotyl spines (= rodlets). The genital sac is well developed, sucker-like, slightly larger than the ventral sucker, muscular, and equipped with two gonotyls on the ventral side of the sac. Gonotyls are protruding pad-like, and the number of rodlets on the left gonotyl is four to five and that on the right gonotyl is 10-11 in two rows. This is the fifth Pygidiopsis species reported in Asia, following P. summa (Japan, Korea, and Vietnam), P. phalacrocorasis (Japan), P. pelecani (China), and Pygidiopsis marivillai (Philippines).
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