The life and death of sponge cells

Sipkema, Detmer; Snijders, Ambrosius P.; Schroën, Carin G. P. H.; Osinga, Ronald; Wijffels, René H.

doi:10.1002/bit.10886

Cited by 23 publications

(13 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Little is known about the rate of ammonium/ammonia gen eration and tolerance in sponges (Osinga et al 1999). According to Sipkema et al (2004), isolated cells of the Medi terranean demosponge Suberites domun cula (Olivi, 1792) were not affected by very high ammonium treatments (up to 25 000 µmol l −1 ; compared to our maximum of 100 µmol l −1 ), but were clearly negatively affected by heat (12 to 28°C). Moreover, the sponge samples originated from a coastal reef that is subject to large fluctuations of seasonal and disturbance-related water quality (e.g.…”

Section: Influence Of Temperature and P Co 2 On Sponge Bioerosionmentioning

confidence: 98%

Effects of ocean acidification and global warming on reef bioerosion—lessons from a clionaid sponge

Wisshak¹,

Schönberg

Form³

et al. 2013

Aquat. Biol.

View full text Add to dashboard Cite

Coral reefs are under threat, exerted by a number of interacting effects inherent to the present climate change, including ocean acidification and global warming. Bioerosion drives reef degradation by recycling carbonate skeletal material and is an important but understudied factor in this context. Twelve different combinations of p CO 2 and temperature were applied to elucidate the consequences of ocean acidification and global warming on the physiological response and bioerosion rates of the zooxanthellate sponge Cliona orientalis -one of the most abundant and effective bioeroders on the Great Barrier Reef, Australia. Our results confirm a significant amplification of the sponges' bioerosion capacity with increasing p CO 2 , which is expressed by more carbonate being chemically dissolved by etching. The health of the sponges and their photosymbionts was not affected by changes in p CO 2 , in contrast to temperature, which had significant negative impacts at higher levels. However, we could not conclusively explain the relationship between temperature and bioerosion rates, which were slightly reduced at both colder as well as warmer temperatures than ambient. The present findings on the effects of ocean acidification on chemical bioerosion, however, will have significant implications for predicting future reef carbonate budgets, as sponges often contribute the lion's share of internal bioerosion on coral reefs.

show abstract

Section: Influence Of Temperature and P Co 2 On Sponge Bioerosionmentioning

confidence: 98%

Effects of ocean acidification and global warming on reef bioerosion—lessons from a clionaid sponge

Wisshak¹,

Schönberg

Form³

et al. 2013

Aquat. Biol.

View full text Add to dashboard Cite

show abstract

“…The rationale to choose these nutritional factors and the two levels for each factor (Table 2) is based on the previous studies (3)(4)(5)(6)(7)(8) as well as the composition analysis of H. perleve (16). The first screening experimental results and their statistical analysis are shown in Tables 1 and 2.…”

Section: Resultsmentioning

confidence: 99%

“…Optical density (5), MTT (4), and fluorescent methods (8) have been applied for quantifying sponge cell density or viability in nutrient studies. MTT assay has been demonstrated as a simple and reliable method for a high-throughput screening of nutrient factors for sponge cell proliferation in 96-well plates (4).…”

Section: Introductionmentioning

confidence: 99%

Formulation of a Basal Medium for Primary Cell Culture of the Marine Sponge Hymeniacidon perleve

Zhao

Zhang

Jin

et al. 2008

Biotechnol Progress

View full text Add to dashboard Cite

Marine sponge cell culture is a potential route for the sustainable production of spongederived bioproducts. Development of a basal culture medium is a prerequisite for the attachment, spreading, and growth of sponge cells in vitro. With the limited knowledge available on nutrient requirements for sponge cells, a series of statistical experimental designs has been employed to screen and optimize the critical nutrient components including inorganic salts (ferric ion, zinc ion, silicate, and NaCl), amino acids (glycine, glutamine, and aspartic acid), sugars (glucose, sorbitol, and sodium pyruvate), vitamin C, and mammalian cell medium (DMEM and RPMI 1640) using MTT assay in 96-well plates. The marine sponge Hymeniacidon perleve was used as a model system. Plackett-Burman design was used for the initial screening, which identified the significant factors of ferric ion, NaCl, and vitamin C. These three factors were selected for further optimization by Uniform Design and Response Surface Methodology (RSM), respectively. A basal medium was finally established, which supported an over 100% increase in viability of sponge cells.

show abstract

“…On the other hand, the private enterprise KliniPharm GmbH is culturing D. avara in the Eastern Mediterranean Sea by holding explants on ropes to obtain avarol for the market (http://www.klinipharm.com). However, only few growth data of those farmed explants have been published [19]. Although at present the most reliable method to culture sponges is in situ aquaculture [20,21] more studies are required to optimize the whole process.…”

Section: Introductionmentioning

confidence: 99%

In Situ Aquaculture Methods for Dysidea avara (Demospongiae, Porifera) in the Northwestern Mediterranean

et al. 2010

View full text Add to dashboard Cite

Marine sponges produce secondary metabolites that can be used as a natural source for the design of new drugs and cosmetics. There is, however, a supply problem with these natural substances for research and eventual commercialisation of the products. In situ sponge aquaculture is nowadays one of the most reliable methods to supply pharmaceutical companies with sufficient quantities of the target compound. In this study, we focus on the aquaculture of the sponge Dysidea avara (Schmidt, 1862), which produces avarol, a sterol with interesting pharmaceutical attributes. The soft consistency of this species makes the traditional culture method based on holding explants on ropes unsuitable. We have tested alternative culture methods for D. avara and optimized the underwater structures to hold the sponges to be used in aquaculture. Explants of this sponge were mounted on horizontal ropes, inside small cages or glued to substrates. Culture efficiency was evaluated by determination of sponge survival, growth rates, and bioactivity (as an indication of production of the target metabolite). While the cage method was the best method for explant survival, the glue method was the best one for explant growth and the rope method for bioactivity.

show abstract

The life and death of sponge cells

Cited by 23 publications

References 58 publications

Effects of ocean acidification and global warming on reef bioerosion—lessons from a clionaid sponge

Effects of ocean acidification and global warming on reef bioerosion—lessons from a clionaid sponge

Formulation of a Basal Medium for Primary Cell Culture of the Marine Sponge Hymeniacidon perleve

In Situ Aquaculture Methods for Dysidea avara (Demospongiae, Porifera) in the Northwestern Mediterranean

Contact Info

Product

Resources

About