The levels of oxidative stress and antioxidant capacity in hibernating Nanorana parkeri

Niu, Youhong; Cao, Wangjie; Zhao, Ye; Zhai, Haotian; Zhao, Yao; Tang, Xiaolong; Chen, Qiang

doi:10.1016/j.cbpa.2018.02.003

Cited by 29 publications

(28 citation statements)

References 55 publications

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“…4A ). Our results resemble recent observations that winter-dormant frogs Nanorana parkeri from the Tibet high mountains present decreased antioxidant enzyme activities in various organs when compared to summer-animals 11 . Even though the POS mechanism is employed by a large proportion of animal species whose redox metabolism was studied, 30% of the analyzed vertebrates are POS-negative species 68 , 69 .…”

Section: Discussionsupporting

confidence: 91%

“…Similarly, GSSG concentration and GSSG/GSH-eq ratio both increase in muscle of lungfish during the first days of estivation 32 . Furthermore, an increase in GSSG concentration also occurs in tissues of estivating land snails 33 and in tissues of hibernating frogs from Tibet mountains 11 .Therefore, a redox imbalance may occur prior to and/or during depressed metabolic states in animals. Even though the tegu intestine experienced a more oxidized state during late autumn, this was not enough to inflict significant oxidative damage (as protein oxidation or lipid peroxidation).…”

Section: Discussionmentioning

confidence: 99%

“…Conversely, markers of oxidative stress (GSSG, carbonyl protein and lipid peroxidation) increased in various organs of N . parkeri frogs during dormancy 11 . Moreover, there was no statistically significant evidence of oxidative stress during arousal from hibernation in juvenile tegus.…”

Section: Discussionmentioning

confidence: 99%

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Subtropical hibernation in juvenile tegu lizards (Salvator merianae): insights from intestine redox dynamics

Moreira

Welker

Campos

et al. 2018

Sci Rep

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Juvenile tegu lizards (Salvator merianae) experience gradual and mild temperature changes from autumn to winter in their habitat. This tropical/subtropical reptile enter a state of dormancy, with an 80% reduction in metabolic rate, that remains almost constant during winter. The redox metabolism in non-mammalian vertebrates that hibernate under such distinguished conditions is poorly understood. We analyzed the redox metabolism in the intestine of juvenile tegus during different stages of their first annual cycle. The effect of food deprivation (in spring) was also studied to compare with fasting during hibernation. Both winter dormancy and food deprivation caused decreases in reduced glutathione levels and glutathione transferase activity. While glutathione peroxidase and glutathione transferase activities decreased during winter dormancy, as well as glutathione (GSH) levels, other antioxidant enzymes (catalase, superoxide dismutase and glutathione reductase) remained unchanged. Notably, levels of disulfide glutathione (GSSG) were 2.1-fold higher in late autumn, when animals were in the process of depressing metabolism towards hibernation. This increased “oxidative tonus” could be due to a disruption in NADPH-dependent antioxidant systems. In dormancy, GSSG and lipid hydroperoxides were diminished by 60–70%. The results suggest that the entrance into hibernation is the main challenge for the redox homeostasis in the intestine of juvenile tegus.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Subtropical hibernation in juvenile tegu lizards (Salvator merianae): insights from intestine redox dynamics

Moreira

Welker

Campos

et al. 2018

Sci Rep

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“…The antioxidant defense system is a highly conserved biochemical mechanism that protects organisms from harmful effects of reactive oxygen species (Birben et al, 2012; Niu et al, 2018). T-AOC is a comprehensive assessment index of antioxidant capacity.…”

Section: Discussionmentioning

confidence: 99%

Improvement of Muscle Quality of Grass Carp (Ctenopharyngodon idellus) With a Bio-Floating Bed in Culture Ponds

et al. 2019

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Muscle quality and the physiological condition of fish are affected greatly by the culture environment. In aquaculture ponds, a bio-floating bed planted with vegetation is often used to improve water quality. This study investigated the growth and muscle quality of grass carp ( Ctenopharyngodon idellus ) cultured in ponds equipped with bio-floating beds. Fish were cultured in two replicated pond groups from May to November. Fish in the first group were cultured in experimental ponds equipped with a bio-floating bed planted with Ipomoea aquatica , whereas fish in the other group were reared in control ponds without a bio-floating bed. Compared with control ponds, the experimental ponds had better water quality with significantly lower concentrations of nitrite and ammonia. Grass carp in the experimental group had greater muscle weight gain, a significantly higher content of crude protein, and a significantly lower crude fat level than fish in the control group. The levels of pH, water-holding capacity, and antioxidant capacity of muscle decreased significantly in the control group compared to the experimental group. Texture profile analysis revealed higher values of hardness, springiness, gumminess, and chewiness and lower values of cohesiveness and resilience of white muscle in the experimental group compared to the control group. The filets of fish in the experimental group also received higher grades in the sensory evaluation of springiness, overall acceptability, aroma, and palatability. These results indicate that growth performance and muscle quality of grass carp were improved by the presence of bio-floating beds in the culture ponds.

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“…Collectively, radical and non-radical species formed by the partial reduction of oxygen are known as reactive oxygen species (ROS) [1]. When ROS overcame the cellular antioxidant defense system, oxidative stress occurs [2], which disturbs cellular metabolism, damages cellular constituents, and triggers the activation of specific signaling pathways [3]. The pathogenesis of several chronic and degenerative human diseases, including cardiovascular, neurodegenerative, and cancerous ones, has been linked to a cellular condition exhibiting oxidative stress [4,5].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Biological Evaluation and Docking Studies of Chalcone and Flavone Analogs as Antioxidants and Acetylcholinesterase Inhibitors

et al. 2019

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Several oxidative processes are related to a wide range of human chronic and degenerative diseases, like Alzheimer’s disease, which also has been related to cholinergic processes. Therefore, search for new or improved antioxidant molecules with acetylcholinesterase activity is essential to offer alternative chemotherapeutic agents to support current drug therapies. A series of chalcone (2a–2k) and flavone (3a–3k) analogs were synthesized, characterized, and evaluated as acetylcholinesterase (AChE) inhibitors, and antioxidant agents using 1,1-diphenyl-2-picrylhydrazyl (DPPH•), 2-2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS•), and β-carotene/linoleic acid bleaching assay. Compounds more active were 3j and 2k in DPPH with EC50 of 1 × 10−8 and 5.4 × 10−3 μg/mL, respectively; 2g and 3i in ABTS (1.14 × 10−2 and 1.9 × 10−3 μg/mL); 2e, 2f, 3f, 2j, and 3j exceeded the α-tocopherol control in the β-carotene assay (98–99% of antioxidant activity). At acetylcholinesterase inhibition assay, flavones were more active than chalcones; the best results were compounds 2d and 3d (IC50 21.5 and 26.8 µg/mL, respectively), suggesting that the presence of the nitro group enhances the inhibitory activity. The docking of these two structures were made to understand their interactions with the AChE receptor. Although further in vivo testing must be performed, our results represent an important step towards the identification of improved antioxidants and acetylcholinesterase inhibitors.

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The levels of oxidative stress and antioxidant capacity in hibernating Nanorana parkeri

Cited by 29 publications

References 55 publications

Subtropical hibernation in juvenile tegu lizards (Salvator merianae): insights from intestine redox dynamics

Subtropical hibernation in juvenile tegu lizards (Salvator merianae): insights from intestine redox dynamics

Improvement of Muscle Quality of Grass Carp (Ctenopharyngodon idellus) With a Bio-Floating Bed in Culture Ponds

Synthesis, Biological Evaluation and Docking Studies of Chalcone and Flavone Analogs as Antioxidants and Acetylcholinesterase Inhibitors

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