The levels of matrix metalloproteinase-9 and neutrophil gelatinase-associated lipocalin in different stages of endometriosis

Durmus, Arzu Bostancı; Cengiz, Sevim; Yılmaz, Hakan; Çandar, Tuba; Gürsoy, Aslı Yarcı; Çağlar, Gamze Sinem

doi:10.1080/01443615.2019.1584889

Cited by 18 publications

(10 citation statements)

References 19 publications

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“…In the absence of the mesothelial barrier, endometrial cells readily adhere to the subperitoneum and form endometriosis. Many studies on endometriosis invasion and fibrosis have also shown the importance of EMT [18][19][20][21]. Therefore, based on all findings, we speculate that ENG may promote cell migration and invasion by regulating EMT phenotype in endometriosis.…”

Section: Discussionsupporting

confidence: 58%

Endoglin promotes cell migration and invasion in endometriosis by regulating EMT

Chen¹,

Wang

Tu³

et al. 2021

Ginekol Pol

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Objectives: This study aims to investigate the expression and role of Endoglin (ENG) in endometriosis (EM). Material and methods:In this study, quantitative real-time polymerase chain reaction, western blot and immunohistochemistry were used to examine the expression of ENG in tissues. Cellular experiments were performed to evaluate the effect of ENG on cellular biological function. Western blot was used to examine the expression of epithelial to mesenchymal transition-related proteins. Results:The expression of ENG was significantly higher in the ectopic endometriotic tissues than that in eutopic endometriotic tissues. Knockdown of ENG inhibited cell viability, migration and invasion, and induced cell apoptosis in hEM15A cells.Additionally, silenced ENG caused increased levels of E-cadherin and decreased levels of N-cadherin, vimentin, MMP-2 and MMP-9.Conclusions: These results confirmed that ENG may be involved in the development of endometriosis by promoting EMT process, revealing a new insight into the pathogenesis of endometriosis and contributing to the exploration of molecular therapeutic strategies against endometriosis.

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Section: Discussionsupporting

confidence: 58%

Endoglin promotes cell migration and invasion in endometriosis by regulating EMT

Chen¹,

Wang

Tu³

et al. 2021

Ginekol Pol

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“…markers, which was observed in the defeat of the peritoneum and ovaries [2,3]. In addition, multidirectional changes in the activity of matrix metalloproteinases, which are observed in endometriosis [4], can lead to changes in the extracellular matrix, which promotes the attachment of endometrial cells with increased adhesion to various components of the extracellular matrix, including type IV collagen, laminin, vitronectin and fibronectin [5]. Further, after adhesion and invasion, the proliferation of endometrial cells and the growth of the ectopic focus will largely depend on angiogenesis, which is mediated by cytokines, leading primarily to an increase in the content of the vascular endothelial growth factor VEGF [6][7][8], and its levels correlate with the severity of the disease [9].…”

Section: Introductionmentioning

confidence: 99%

Organization of the Cytoskeleton in Ectopic Foci of the Endometrium with Rare Localization

Toniyan

Povorova²,

Gorbacheva³

et al. 2021

Biomedicines

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(1) Background: Endometriosis is a common pathology of the female reproductive system, often accompanied by pain and decreased fertility. However, its pathogenesis has not been sufficiently studied regarding the role of the cytoskeleton. In this study, we describe two clinical cases involving rare localization of extragenital endometriosis (umbilicus) and compare them with genital endometriosis of different localization (ovaries and uterus), as well as eutopic endometrium obtained with separate diagnostic curettage without confirmed pathology. (2) Methods: The relative content of actin and tubulin cytoskeleton proteins was determined by Western blotting, and the expression of genes encoding these proteins was determined by RT-PCR in the obtained intraoperative biopsies. The content of 5hmC was estimated by dot blot experiments, and the methylase/demethylase and acetylase/deacetylase contents were determined. (3) Results: The obtained results indicate that the content of the actin-binding protein alpha-actinin1 significantly increased (p < 0.05) in the groups with endometriosis, and this increase was most pronounced in patients with umbilical endometriosis. In addition, both the mRNA content of the ACTN1 gene and 5hmC content increased. It can be assumed that the increase in 5hmC is associated with a decrease in the TET3 demethylase content. Moreover, in the groups with extragenital endometriosis, alpha- and beta-tubulin content was decreased (p < 0.05) compared to the control levels. (4) Conclusions: In analyzing the results, further distance of ectopic endometrial foci from the eutopic localization may be associated with an increase in the content of alpha-actinin1, probably due to an increase in the expression of its gene and an increase in migration potential. In this case, a favorable prognosis can be explained by a decrease in tubulin content and, consequently, a decrease in the rate of cell division.

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“…Similarly, such inhibitory effects of Miodesin TM were also seen at mRNA levels. Studies suggest that MMPs are associated with the establishment and progression of endometriosis and their levels appear to be increased in ectopic endometriotic tissues [ 5 ]. MMP-2 and MMP-9 have been extensively studied in the scope of endometriosis, mainly because they are present at high levels in the peritoneal fluid of patients with endometriosis [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…The pro-inflammatory cascade in endometriosis triggers changes in cell proliferation, adhesion, and migration of endometrial tissue, which are fundamental for the success of ectopic implantation [ 3 ]. These events are also essential for induction of endometrial lesions in the peritoneal cavity, which have a key role of matrix metalloproteinase (MMP), such as MMP-2 [ 4 ], MMP-9 [ 5 ], and VEGF [ 6 ]. Furthermore, such a process occurs due to an orchestration by nuclear factor kappa beta (NF-kβ) [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

MiodesinTM Positively Modulates the Immune Response in Endometrial and Vaginal Cells

Oliveira

Polonini²,

Marcucci

2022

Molecules

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Endometriosis presents high prevalence and its physiopathology involves hyperactivation of endometrial and vaginal cells, especially by bacteria. The disease has no cure and therapies aiming to inhibit its development are highly desirable. Therefore, this study investigated whether MiodesinTM (10 µg/mL = IC80; 200 µg/mL = IC50), a natural compound constituted by Uncaria tomentosa, Endopleura uchi, and astaxanthin, could exert anti-inflammatory and anti-proliferative effects against Lipopolysaccharides (LPS) stimulation in endometrial and Candida albicans vaginal cell lines. VK2 E6/E7 (vaginal) and KLE (epithelial) cell lines were stimulated with Candida albicans (1 × 107 to 5 × 107/mL) and LPS (1 μg/mL), respectively. MiodesinTM inhibited mRNA expression for Nuclear factor kappa B (NF-κB), ciclo-oxigenase 1 (COX-1), and phospholipase A2 (PLA2), beyond the C–C motif chemokine ligand 2 (CCL2), CCL3, and CCL5 in VK2 E6/E7 cells (p < 0.05). In addition, the inhibitory effects of both doses of MiodesinTM (10 µg/mL and 200 µg/mL) resulted in reduced secretion of interleukin-1β (IL-1β), IL-6, IL-8, tumor necrosis factor α (TNF-α) (24 h, 48 h, and 72 h) and CCL2, CCL3, and CLL5 (p < 0.05) by VK2 E6/E7 cells. In the same way, COX-1 MiodesinTM inhibited LPS-induced hyperactivation of KLE cells, as demonstrated by reduced secretion of IL-1β, IL-6, IL-8, TNF-α (24 h, 48 h, and 72 h) and CCL2, CCL3, and CLL5 (p < 0.05). Furthermore, MiodesinTM also inhibited mRNA expression and secretion of matrix metalloproteinase-2 (MMP-2), MMP-9, and vascular endothelial growth factor (VEGF), which are key regulators of invasion of endometrial cells. Thus, the study concludes that MiodesinTM presents beneficial effects in the context of endometriosis, positively affecting the inflammatory and proliferative response.

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The levels of matrix metalloproteinase-9 and neutrophil gelatinase-associated lipocalin in different stages of endometriosis

Cited by 18 publications

References 19 publications

Endoglin promotes cell migration and invasion in endometriosis by regulating EMT

Endoglin promotes cell migration and invasion in endometriosis by regulating EMT

Organization of the Cytoskeleton in Ectopic Foci of the Endometrium with Rare Localization

MiodesinTM Positively Modulates the Immune Response in Endometrial and Vaginal Cells

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