The Levels of Boron‐Uptake Proteins in Roots are Correlated with Tolerance to Boron Stress in Barley

Abstract

Abbreviations: ELISA, enzyme-linked immunosorbent assay; NIP, NOD26-like intrinsic proteins; QTL, quantitative trait loci.

Effects of Heat Stress on Plant-Nutrient Relations: An Update on Nutrient Uptake, Transport, and Assimilation

“…Surprisingly, most studies on nutrient uptake in plants were conducted only by changing the nutrient concentrations either to deficiency or toxicity levels. Only a few studies are available on the status of nutrient uptake proteins during abiotic stress [38,62,72,[77][78][79][80]. In all these studies, relative levels of the major nutrient-uptake proteins (NRT1, NRT2, AMT1, PHT1, BOR1, NIP5;1, and FRO1) per unit total root protein were quantified using ELISA (enzyme-linked immunosorbent assay) or Western blots [62,72] using protein-specific antibodies.…”

“…In all these studies, relative levels of the major nutrient-uptake proteins (NRT1, NRT2, AMT1, PHT1, BOR1, NIP5;1, and FRO1) per unit total root protein were quantified using ELISA (enzyme-linked immunosorbent assay) or Western blots [62,72] using protein-specific antibodies. Polyclonal antibodies were designed that were specific to conserved domains of certain nutrient-uptake proteins across species [62,72,77]. Of the above-mentioned seven studies, only one study reported a change in nutrient-uptake protein due to heat stress alone [38].…”