The  level of transforming growth factor beta as a possible predictor of cyclophosphamide response in children with steroid-resistant nephrotic syndrome

Widiasta, Ahmedz; Wahyudi, Kurnia; Sribudiani, Yunia; Rachmadi, Dedi

doi:10.37796/2211-8039.1205

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…Also, TGF-β has an essential role in proteinuria and chronic kidney disease (CKD) progression. 27 , 28 Previous studies showed that the apoptosis of glomerular cells has been linked to DN. 29 Oxidative stress is believed to be a factor influencing the pathogenesis of DN.…”

Section: Discussionmentioning

confidence: 99%

Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway

Mai¹,

He²,

Chen³

et al. 2023

DMSO

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Background Profilin-1 (PFN1) regulates the dynamic balance of actin and plays an important role in cell functions as a hub protein in signaling molecule interaction networks. Dysregulation of PFN1 is related to pathologic kidney diseases. Diabetic nephropathy (DN) was recently reported as an inflammatory disorder, however, the molecular mechanisms of PFN1 in DN remain unclear. Therefore, the present study was conducted to explore the molecular and bioinformatic characteristics of PFN1 in DN. Methods Bioinformatics analyses were performed on the chip of database in DN kidney tissues. A cellular model of DN was established in human renal tubular epithelial cells (HK-2) induced by high glucose. The PFN1 gene was overexpressed or knocked-down to investigate its function in DN. Flow cytometry was used to detect cell proliferation and apoptosis. PFN1 and proteins in the related signaling pathways were evaluated by Western blotting. Results The expression of PFN1 was significantly increased in DN kidney tissues ( P < 0.001) and was correlated with a high apoptosis-associated score (Pearson’s correlation = 0.664) and cellular senescence-associated score (Pearson’s correlation = 0.703). PFN1 protein was mainly located in cytoplasm. Overexpression of PFN1 promoted apoptosis and blocked the proliferation of HK-2 cells treated with high levels of glucose. Knockdown of PFN1 led to the opposite effects. Additionally, we found that PFN1 was correlated with the inactivation of the Hedgehog signaling pathway in HK-2 cells treated with high levels of glucose. Conclusion PFN1 might play an integral role in the regulation of cell proliferation and apoptosis during DN development by activating the Hedgehog signaling pathway. This study provided molecular and bioinformatic characterizations of PFN1, and contributed to the understanding of the molecular mechanisms leading to DN.

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Section: Discussionmentioning

confidence: 99%

Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway

Mai¹,

He²,

Chen³

et al. 2023

DMSO

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“…The other hypothesis of AKI is that several pro-inflammatory are released in the clinically severe COVID-19 manifestation, resulting in podocyte collapse, apoptosis, and even glomerular fibrosis [3], [5], [8], [17]. The AKI aggravate the proteinuria by releasing transforming growth factor beta (TGF-β) [18].…”

Section: Discussionmentioning

confidence: 99%

The Association between Kidney Function and the Severity of COVID-19 in Children

Rachmadi

Widiasta²,

Sukandar³

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: Kidney manifestations are life-threatening conditions, such as end-stage kidney disease, notably when caused by viral infections. The severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2), is an emerging global health problem, potentially affecting all organs, including the kidney. Most reports on kidney manifestations were focused on the adult and elderly population but limited on children. AIM: This study aims to analyze the association between kidney function and the severity of the disease of pediatric patients suffering from COVID-19. METHODS: From March 2020 to March 2021, an observational analytic study was conducted in Hasan Sadikin General Hospital, Bandung, Indonesia. The demographic data, clinical signs, laboratory results, and notable kidney function were analyzed, and based on its clinical appearance the disease was classified as severe and non-severe. Subsequently, the Mann–Whitney test for nonparametric was used to analyze the collected data. RESULTS: In this study, 40 COVID-19 children were selected as the subjects, and the median estimated glomerular filtration rate (eGFR) value in the severe group was discovered to be lower (88.2 mL/min/1.73 m2) compared to the non-severe (124.4 mL/min/1.73 m2), it was statistically significant (p = 0.041). There was no difference in hemoglobin and leukocyte values between the two groups (p > 0.05). Furthermore, the CRP and NLR results showed no difference between the groups (p > 0.05). CONCLUSION: A severe SARS-CoV-2 infection correlates with kidney function, which was manifested by a lower median eGFR value in the severe compared to the non-severe group.

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“…Furthermore, some studies have linked high levels of transforming growth factor-β as a prognostic factor for poor response to cyclophosphamide in steroidresistant nephrotic syndrome (SRNS). Despite the fact that steroid-resistant nephrotic syndrome is currently an important cause of progressive kidney disease, we had no cases in our sample [20,21]. The findings of our study and the pediatric and the adult population from the literature the benefits of percutaneous treatment were similar on both: TRAS group (intervention) showed similar evolutions with percutaneous treatment, such as an improvement in blood pressure levels, renal graft survival, decrease in the number of antihypertensive drugs [1][2][3][4]6,7,14,[16][17][18][19]22,23].…”

Section: Plos Onementioning

confidence: 99%

Retrospective analysis of percutaneous intervention of the renal artery in transplanted kidneys in children and adolescents at a tertiary public hospital

Faccinetto,

Santos,

Taguchi

et al. 2024

PLoS ONE

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Background This study evaluated the long-term effects of percutaneous intervention in children and adolescents with transplant renal artery stenosis (TRAS). Methods Twenty patients had significant stenosis (>50%) and underwent percutaneous transluminal angioplasty (PTA/stenting) (TRAS group-intervention); 14 TNS (non-significant group -control) patients did not have significant stenosis (≤50%) and were treated clinically. The combined primary endpoints were death from all causes and late graft failure. The secondary endpoints were serum creatinine (SCr), systolic blood pressure (SBP), and diastolic blood pressure (DBP). Results No statistically significant difference was found between TRAS—Intervention(N = 20) and TNS groups—Control (N = 14) for these clinical parameters: deaths, 1 (5.0%) vs. 0 (0.0%) (p = 1.000) and graft loss, 4 (20.0%) vs. 2 (14.3%) (p = 1.000). For the secondary endpoints, after 1 month and 1 year the values of SCr, SBP, and DBP were similar between the two groups but not statistically significant. Discussion In the TRAS group (intervention), the stent implantation was beneficial for treating refractory hypertension and reducing blood pressure (BP) in children and adolescents. Despite the outcomes being similar in the two groups, it can be inferred that the patients in the TRAS group (intervention) would have had a worse outcome without the percutaneous intervention. Conclusion TRAS treatment with stenting can be considered for children and adolescents. Because the sample in the present study comprised of only a specific population, further studies are needed for generalization. Trial registration The trial was registered at clinictrials.gov with trial registration number NCT04225338.

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The level of transforming growth factor beta as a possible predictor of cyclophosphamide response in children with steroid-resistant nephrotic syndrome

Cited by 8 publications

References 34 publications

Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway

Profilin1 Promotes Renal Tubular Epithelial Cell Apoptosis in Diabetic Nephropathy Through the Hedgehog Signaling Pathway

The Association between Kidney Function and the Severity of COVID-19 in Children

Retrospective analysis of percutaneous intervention of the renal artery in transplanted kidneys in children and adolescents at a tertiary public hospital

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