The level of endogenous DNA damage in lymphocytes isolated from blood is associated with the fluctuation of 17beta-estradiol concentration in the follicular phase of healthy young women.

Kapiszewska, Maria; Kalemba, Malgorzata; Grzesiak, Aneta; Kocemba, Kinga A.

doi:10.18388/abp.2005_3469

Cited by 10 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies have found non-significant differences, 24,48 whereas others have found a positive correlation between DNA damage levels (in the late follicular phase) and 17β-estradiol levels. 22 Furthermore, significant differences in DNA damage and repair among the menstrual cycle phases showed that the pre-ovulatory stage had the lowest DNA repair capacity. This variability in the observed results…”

Section: Phases Of the Menstrual Cycle Trimesters Of Pregnancymentioning

confidence: 99%

“…21 DNA integrity is a critical homeostatic condition for survival and reproduction, in which DNA is the template for a new individual. In addition, DNA damage and repair levels are essential in reproductive processes, including the menstrual cycle [22][23][24] and pregnancy. 25 The detailed mechanisms that explain the association between ANA and the pathophysiology and development of SLE remain to be defined.…”

Section: Introductionmentioning

confidence: 99%

“…DNA integrity is a critical homeostatic condition for survival and reproduction, in which DNA is the template for a new individual. In addition, DNA damage and repair levels are essential in reproductive processes, including the menstrual cycle 22–24 and pregnancy 25 …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dynamics of DNA damage response markers, RAD51 and XRCC1, throughout the menstrual cycle and pregnancy in healthy women. Increment during pregnancy and relation to a low titer of antinuclear antibodies

González-Chávez

Anda

Mireles-Canales

et al. 2022

American J Rep Immunol

View full text Add to dashboard Cite

Problem: Antinuclear antibodies (ANA) potentially play a role in the pathogenesis of connective tissue diseases (CTDs); however, they are also detected in healthy individuals. Our understanding of the physiological and pathological origin of ANA is incomplete. Both; female reproductive processes and deficiencies in DNA damage response (DDR) have been independently associated with ANA production. However, the link between these two factors and the presence of ANA is unclear Method of study: A cohort study was conducted on 87 non-pregnant and 51 healthy pregnant women. ANA and the DDR markers X-ray repair cross-complementing 1 (XRCC1) and recombinase (RAD51) were quantified in sera by enzyme-linked immunosorbent assay. Additionally, ANA was detected by indirect immunofluorescence in HEp-2 cells in 1:40 diluted sera during the three phases of the menstrual cycle in non-pregnant women and every trimester in pregnant women Results:The prevalence of ANA+ in healthy women was 26.1%, with no significant differences between pregnant and non-pregnant women. ANA and DDR marker levels did not vary among the three phases of the menstrual cycle or the three trimesters of pregnancy. However, DDR marker levels were higher in pregnant women than in non-pregnant women (p < .001) and were found to be higher in ANA+ women (XRCC1, p = .025; RAD51, p = .006) Conclusions:The menstrual cycle and pregnancy did not influence the levels of DDR markers or ANA in healthy women; however, the DDR was higher in pregnancy and ANA+ women. The results suggest a potential role of DDR in the pathophysiology of ANA.

show abstract

Section: Phases Of the Menstrual Cycle Trimesters Of Pregnancymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dynamics of DNA damage response markers, RAD51 and XRCC1, throughout the menstrual cycle and pregnancy in healthy women. Increment during pregnancy and relation to a low titer of antinuclear antibodies

González-Chávez

Anda

Mireles-Canales

et al. 2022

American J Rep Immunol

View full text Add to dashboard Cite

show abstract

Lack of genotoxicity induced by endogenous and synthetic female sex hormones in peripheral blood cells detected by alkaline comet assay

Braz

Salvadori

2007

Environ and Mol Mutagen

View full text Add to dashboard Cite

The etiology of hormone-induced cancers has been considered to be a combination of genotoxic and epigenetic events. Currently, the Comet assay is widely used for detecting genotoxicity because it is relatively simple, sensitive, and capable of detecting various kinds of DNA damage. The present study evaluates the genotoxic potential of endogenous and synthetic sex hormones, as detected by the Comet assay. Blood cells were obtained from 12 nonsmoking and 12 smoking women with regular menstrual cycles and from 12 nonsmoking women taking low-dose oral contraceptives (OC). Peripheral blood samples were collected at three phases of the menstrual cycle (early follicular, mean follicular, and luteal phases), or at three different moments of oral contraceptive intake. Three blood samples were also collected from 12 healthy nonsmoking men, at the same time as oral contraceptive users. Results showed no significant difference in the level of DNA damage among the three moments of the menstrual cycle either in nonsmoking and smoking women, or between them. No significant difference in DNA damage was also observed among oral contraceptive users, nonusers, and men. Together, these data indicate lack of genotoxicity induced by the physiological level of the female sex hormones and OC as assessed by the alkaline Comet assay. In conclusion, normal fluctuation in endogenous sex hormones and use of low-doses of oral contraceptive should not interfere with Comet assay data when this technique is used for human biomonitoring.

show abstract

High anesthetic (isoflurane) indoor pollution is associated with genetic instability, cytotoxicity, and proliferative alterations in professionals working in a veterinary hospital

Figueiredo

Aun

Souza

et al. 2022

Environ Sci Pollut Res

View full text Add to dashboard Cite

The level of endogenous DNA damage in lymphocytes isolated from blood is associated with the fluctuation of 17beta-estradiol concentration in the follicular phase of healthy young women.

Cited by 10 publications

References 23 publications

Dynamics of DNA damage response markers, RAD51 and XRCC1, throughout the menstrual cycle and pregnancy in healthy women. Increment during pregnancy and relation to a low titer of antinuclear antibodies

Dynamics of DNA damage response markers, RAD51 and XRCC1, throughout the menstrual cycle and pregnancy in healthy women. Increment during pregnancy and relation to a low titer of antinuclear antibodies

Lack of genotoxicity induced by endogenous and synthetic female sex hormones in peripheral blood cells detected by alkaline comet assay

High anesthetic (isoflurane) indoor pollution is associated with genetic instability, cytotoxicity, and proliferative alterations in professionals working in a veterinary hospital

Contact Info

Product

Resources

About