The level of DLDB/CHIP controls the activity of the LIM homeodomain protein Apterous: evidence for a functional tetramer complex in vivo

Rincón-Limas, Diego E.; Lu, Cheng-Hsin; Canal, Inmaculada; Botas, Juan

doi:10.1093/emboj/19.11.2602

Cited by 48 publications

(58 citation statements)

References 43 publications

(75 reference statements)

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“…4F). As ap is expressed in all notal cells after the second larval period (DiazBenjumea and Cohen, 1993;Rincon-Limas et al, 2000) and precedes eyg expression, we believe that inducing the activity of eyg early in notum development programmes the whole notum to develop as the eyg/toe domain, thus preventing the formation of scutellum (see Discussion). The transformations observed in clones of eyg-expressing cells (data not shown) fit well with those described with the Gal4 lines.…”

Section: Misexpression Of Eyg and Toementioning

confidence: 84%

The Pax-homeobox geneeyegoneis involved in the subdivision of the thorax ofDrosophila

2003

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The eyegone (eyg) gene is known to be involved in the development of the eye structures of Drosophila. We show that eyg and its related gene, twin of eyegone (toe), are also expressed in part of the anterior compartment of the adult mesothorax (notum). We report experiments concerning the role of these genes in the notum. In the absence of eyg function the anterior-central region does not develop, whereas ectopic activity of either eyg or toe induces the formation of the anterior-central pattern in the posterior or lateral region of the notum. These results demonstrate that eyg and toe play a role in the genetic subdivision of the notum, although the experiments indicate that eyg exerts the principal function. However, by itself the Eyg product cannot induce the formation of notum patterns; its thoracic function requires co-expression with the Iroquois (Iro) genes. We show that the restriction of eyg activity to the anterior-central region of the wing disc is achieved by the antagonistic regulatory activities of the Iro and pnr genes, which promote eyg expression, and those of the Hh and Dpp pathways, which act as repressors. We argue that eyg is a subordinate gene of the Iro genes, and that pnr mediates their thoracic patterning function. The activity of eyg gives rise to a new notum subdivision that acts upon the pre-extant one generated by the Iro genes and pnr. As a result the notum becomes subdivided into four distinct genetic domains.

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Section: Misexpression Of Eyg and Toementioning

confidence: 84%

The Pax-homeobox geneeyegoneis involved in the subdivision of the thorax ofDrosophila

2003

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“…The tetrameric model for LIM-HD function based on studies of Ap, dLMO, and CHIP, requires two Ap molecules binding a dimer of CHIP, a process that dLmo proteins can interfere with by competing for binding to CHIP (Fernandez-Funez et al, 1998;Milan and Cohen, 1999;Rincon-Limas et al, 2000). To determine the combinations in which members of the mouse LIM-HD, Lmo, and Clim families may function in the cortex, a comparison of the spatial overlap in expression of these genes is essential.…”

Section: Discussionmentioning

confidence: 99%

Dynamic spatiotemporal expression of LIM genes and cofactors in the embryonic and postnatal cerebral cortex

Bulchand

Subramanian

Tole

2003

Developmental Dynamics

120

132

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“…The developing wing of Drosophila has proven a tractable system in which to study the function of complexes formed by LIM-HD proteins and their co-factors (Fernandez-Funez et al, 1998;Milan et al, 1998;Shoresh et al, 1998;Zeng et al, 1998;Milan and Cohen, 1999;Rincon-Limas et al, 2000;. The wing imaginal disc is divided into distinct lineage-restricted compartments along both the anteroposterior and dorsoventral (DV) axes.…”

Section: Introductionmentioning

confidence: 99%

Ssdp proteins bind to LIM-interacting co-factors and regulate the activity of LIM-homeodomain protein complexes in vivo

2003

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LIM-homeodomain transcription factors control a variety of developmental processes, and are assembled into functional complexes with the LIM-binding co-factor Ldb1 (in mouse) or Chip (in Drosophila). We describe the identification and characterization of members of the Ssdp family of proteins, which we show to interact with Ldb1 and Chip. The N terminus of Ssdp is highly conserved among species and binds a highly conserved domain within Ldb1/Chip that is distinct from the domains required for LIM binding and self-dimerization. In Drosophila, Ssdp is expressed in the developing nervous system and imaginal tissues, and it is capable of modifying the in vivo activity of complexes comprised of Chip and the LIM-homeodomain protein Apterous. Null mutations of the ssdp gene are celllethal in clones of cells within the developing wing disc. However, clones mutant for a hypomorphic allele give rise to ectopic margins, wing outgrowth and cell identity defects similar to those produced by mutant clones of Chip or apterous. Ssdp and Ldb/Chip each show structural similarity to two Arabidopsis proteins that cooperate with one another to regulate gene expression during flower development, suggesting that the molecular interactions between Ssdp and Ldb/Chip proteins are evolutionarily ancient and supply a fundamental function in the regulated control of transcription.

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The level of DLDB/CHIP controls the activity of the LIM homeodomain protein Apterous: evidence for a functional tetramer complex in vivo

Cited by 48 publications

References 43 publications

The Pax-homeobox geneeyegoneis involved in the subdivision of the thorax ofDrosophila

The Pax-homeobox geneeyegoneis involved in the subdivision of the thorax ofDrosophila

Dynamic spatiotemporal expression of LIM genes and cofactors in the embryonic and postnatal cerebral cortex

Ssdp proteins bind to LIM-interacting co-factors and regulate the activity of LIM-homeodomain protein complexes in vivo

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