The level of cAMP-dependent protein kinase A activity strongly affects osmotolerance andosmo-instigated gene expression changes inSaccharomyces cerevisiae

Norbeck, Joakim; Blomberg, Anders

doi:10.1002/(sici)1097-0061(20000130)16:2<121::aid-yea511>3.0.co;2-a

Cited by 79 publications

(62 citation statements)

References 48 publications

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“…PKA signaling acts as a negative regulator in most types of stress. Consistent with this, a deletion mutation in BCY1 or overexpression of the PKA catalytic subunit reduces yeast viability following high salinity shock (Norbeck and Blomberg 2000). The observed up-regulation of Bcy1p (Fig.…”

Section: Signalingsupporting

confidence: 79%

Yeast translational response to high salinity: Global analysis reveals regulation at multiple levels

Melamed

Pnueli²,

Arava³

2008

RNA

102

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Genome-wide studies of steady-state mRNA levels revealed common principles underlying transcriptional changes in response to external stimuli. To uncover principles that govern other stages of the gene-expression response, we analyzed the translational response and its coordination with transcriptome changes following exposure to severe stress. Yeast cells were grown for 1 h in medium containing 1 M NaCl, which elicits a maximal but transient translation inhibition, and nonpolysomal or polysomal mRNA pools were subjected to DNA-microarray analyses. We observed a strong repression in polysomal association for most mRNAs, with no simple correlation with the changes in transcript levels. This led to an apparent accumulation of many mRNAs as a nontranslating pool, presumably waiting for recovery from the stress. However, some mRNAs demonstrated a correlated change in their polysomal association and their transcript levels (i.e., potentiation). This group was enriched with targets of the transcription factors Msn2/Msn4, and the translational induction of several tested mRNAs was diminished in an Msn2/Msn4 deletion strain. Genome-wide analysis of a strain lacking the high salinity response kinase Hog1p revealed that the group of translationally affected genes is significantly enriched with motifs that were shown to be associated with the AREbinding protein Pub1. Since a relatively small number of genes was affected by Hog1p deletion, additional signaling pathways are likely to be involved in coordinating the translational response to severe salinity stress.

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Section: Signalingsupporting

confidence: 79%

Yeast translational response to high salinity: Global analysis reveals regulation at multiple levels

Melamed

Pnueli²,

Arava³

2008

RNA

102

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“…GCY1 (galactose-inducible crystallin-like yeast protein) was found to encode a glycerol dehydrogenase involved in osmoregulation and osmotolerance of Saccharomyces cerevisiae (28,29). We have investigated transcriptional regulation of the galactose-inducible gene, GCY1, which is activated by the specific regulator, Gal4p.…”

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confidence: 99%

Permanent Nucleosome Exclusion from the Gal4p-inducible YeastGCY1 Promoter

Angermayr

Bandlow

2003

Journal of Biological Chemistry

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The promoter of the galactose-inducible yeast GCY1 gene allows high rates of basal transcription and is kept free of nucleosomes regardless of growth conditions. The general regulatory factor, Reb1p, as well as the nucleotide sequence of a single Gal4p-binding site, structurally cooperate to exclude nucleosomes from about 480 bp of DNA that spans the UAS GAL , the Reb1p-binding site, the TATA-box, and the transcriptional initiation sites. Gal4p, which induces transcription of GCY1 about 25-fold in the presence of galactose, is not required for the alteration in chromatin configuration in the promoter upstream region since the hypersensitive site is unchanged when Gal4p is inactive or absent. As soon as either the Reb1p-binding site or the UAS GAL or both are mutated, nucleosomes slip into the promoter of GCY1 paralleled by a reduction of basal transcription activity to about 30% in either single mutant and to <10% in the double mutant. In the mutant of the Reb1p-binding site, induction by galactose/Gal4p restores a nucleosome-free state to an extent resembling the GCY1 wild-type promoter, showing that, in principle, activated Gal4p can exclude nucleosomes on its own. Northern blots of GCY1 transcripts confirm that Reb1p modulates basal transcription and has little influence on the galactose-induced state.

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“…More specifically, efficient adaptation to osmotic stress depends on the cellular PKA activity (17) that is inhibited by salt stress (18). One important mechanism by which PKA influences gene expression seems to be the modulation of nucleo-cytoplasmic trafficking of transcription factors.…”

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confidence: 99%

Multiple Levels of Control Regulate the Yeast cAMP-response Element-binding Protein Repressor Sko1p in Response to Stress

Pascual-Ahuir¹,

Posas²,

Serrano³

et al. 2001

Journal of Biological Chemistry

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The Sko1p transcriptional repressor regulates a subset of osmoinducible stress defense genes in Saccharomyces cerevisiae by binding to cAMP-responsive elements. We have reported previously that in response to stress Sko1p is phosphorylated by the stress-activated Hog1p mitogenactivated protein kinase, which disrupts its interaction with the Ssn6p⅐Tup1p corepressor. Here we report that other mechanisms are essential for the regulation of the Sko1p repressor activity upon stress. The nuclear localization of Sko1p depends on the stress-inhibited protein kinase A (PKA). Sko1p is localized in the nucleus of unstressed cells, and it redistributes to the cytosol upon severe salt stress (1 M NaCl). Yeast mutants with low PKA activity localize Sko1p to the cytoplasm in the absence of stress and exhibit deregulated expression of cAMPresponsive element-regulated genes. The central part (315-486) of Sko1p, containing the PKA phosphorylation sites and the basic domain-leucine zipper domain, is essential for its nuclear localization. Salt-induced export of Sko1p from the nucleus is independent of Hog1p and of the Bcy1p regulatory subunit of PKA. Furthermore, phosphorylation by PKA slightly enhanced DNA binding affinity of Sko1p in vitro, whereas Sko1p dimerization in vivo is not regulated by stress. Sko1p repressor activity is associated to its binding to the Ssn6p⅐Tup1p complex. Interestingly, the Sko1p NH 2 terminus (1-315), containing the Hog1p phosphorylation sites, associates in vivo with Tup1p in the absence of Ssn6p, suggesting that Sko1p represses gene transcription by interacting directly with the Tup1p subunit of the Ssn6p⅐Tup1p complex.

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The level of cAMP-dependent protein kinase A activity strongly affects osmotolerance andosmo-instigated gene expression changes inSaccharomyces cerevisiae

Cited by 79 publications

References 48 publications

Yeast translational response to high salinity: Global analysis reveals regulation at multiple levels

Yeast translational response to high salinity: Global analysis reveals regulation at multiple levels

Permanent Nucleosome Exclusion from the Gal4p-inducible YeastGCY1 Promoter

Multiple Levels of Control Regulate the Yeast cAMP-response Element-binding Protein Repressor Sko1p in Response to Stress

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