The level of blood plasma mitochondrial DNA upon acute myocardium damage in experiment

Abstract

The aim of the present investigation is to study the level of plasma mtDNA as a potential marker of cardiomyocyte damage in 2 and 4 h after subcutaneous injection of adrenaline and during the formed morphological alterations of the myocardium (3 days). Methods. Real time PCR. Male Wistar rats were used as the experimental animals. Results. It was shown that during the increase in the activity of cytolysis biomarkers, at the first hours after adrenaline injection, no reliable increase is observed in the level of free circulating blood mtDNA. A tendency of 2.5-fold increase in this parameter was established at the third day after adrenaline injection during the development of acute inflammatory process in the myocardium. On the whole, further researches are needed on the dynamics of mtDNA level upon acute damage of the myocardium in experimental and clinical investigations for unbiased estimation of the prospects of using the parameter in laboratory diagnostics

The level of free circulating mitochondrial DNA in blood as predictor of death in case of acute coronary syndrome

“…It was already explored the dynamics of the level of free circulating blood plasma mtDNA in acute myocardial ischemia and observed the increase in blood plasma mtDNA concentration [5–7]. Recently, we found the raise of concentration of free circulating mitochondrial DNA in blood after injection of adrenaline that caused the development of multiple small focal myocardial ischemia in Wistar rats [8, 9]. Recent observations [10] reveal the molecular mechanism behind the development of acute heart failure in animal models, where mitochondrial DNA is an important pathogenic factor.…”

Mitochondrial Dysfunction at Atherosclerosis and Myocardial Infarction: Molecular and Cytochemical Cell-Markers

“…Тем не менее, в настоящее время не выявлены биомаркеры, связующие со-стояние митохондрий и процессы гибели клетки при инфаркте миокарда и атеросклерозе. Одним из перспективных показателей разрушения, стрессиро-вания клеток и, возможно, состояния митохондрий является свободно циркулирующая митохондриаль-ная ДНК (мтДНК) крови [1,4,7], которую относят к «молекулярным паттернам риска» (danger associated molecular patterns, DAMPs) [8]. Таким образом, высо-кую актуальность для разработки диагностических и лечебных технологий представляет изучение данных цитологических и молекулярных показателей на ран-них сроках развития дислипопротеидемии, а также в динамике острого ишемического повреждения мио-карда, что явилось целью настоящего исследования.…”