The level of antimicrobial resistance of sewage isolates is higher than that of river isolates in different Escherichia coli lineages

Ogura, Yoshitoshi; Ueda, Takuya; Nukazawa, Kei; Hiroki, Hayate; Xie, Hengkun; Arimizu, Yoko; Hayashi, Tetsuya; Suzuki, Yoshihiro

doi:10.1038/s41598-020-75065-x

Cited by 15 publications

(12 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the absence of selective pressure from sulfonamide, sulfonamide-resistant bacteria may remain stable in the environment for at least 5–10 years longer than sulfonamide itself ( 100 ). The sulfonamide-resistant phenotypes that carried no resistant determinants was most likely due to the acquisition of other mechanisms (e.g., mutations in the chromosomal DHPS gene flop ), as environmental E. coli strains may acquire genetically unrecognized resistance mechanisms more frequently compared to clinical E. coli strains ( 101 , 102 ).…”

Section: Resultsmentioning

confidence: 99%

Prevalence and Diversity of Antibiotic Resistant Escherichia coli From Anthropogenic-Impacted Larut River

Bong

Low

Chai

et al. 2022

Front. Public Health

View full text Add to dashboard Cite

Aquatic environments, under frequent anthropogenic pressure, could serve as reservoirs that provide an ideal condition for the acquisition and dissemination of antibiotic resistance genetic determinants. We investigated the prevalence and diversity of antibiotic-resistant Escherichia coli by focusing on their genetic diversity, virulence, and resistance genes in anthropogenic-impacted Larut River. The abundance of E. coli ranged from (estimated count) Est 1 to 4.7 × 105 (colony-forming units per 100 ml) CFU 100 ml−1 to Est 1 to 4.1 × 105 CFU 100 ml−1 with phylogenetic group B1 (46.72%), and A (34.39%) being the most predominant. The prevalence of multiple antibiotic resistance phenotypes of E. coli, with the presence of tet and sul resistance genes, was higher in wastewater effluents than in the river waters. These findings suggested that E. coli could be an important carrier of the resistance genes in freshwater river environments. The phylogenetic composition of E. coli and resistance genes was associated with physicochemical properties and antibiotic residues. These findings indicated that the anthropogenic inputs exerted an effect on the E. coli phylogroup composition, diversification of multiple antibiotic resistance phenotypes, and the distribution of resistance genes in the Larut River.

show abstract

Section: Resultsmentioning

confidence: 99%

Prevalence and Diversity of Antibiotic Resistant Escherichia coli From Anthropogenic-Impacted Larut River

Bong

Low

Chai

et al. 2022

Front. Public Health

View full text Add to dashboard Cite

show abstract

“…Available studies on AMR and the environment are predominantly from high resource settings [ 7 , 8 , 9 ], with a gap in knowledge from low and middle-income settings [ 10 , 11 ]. AMR is facilitated by the discharge of poorly treated human and animal waste as well as antimicrobials from pharmaceutical industries and health facilities into the environment [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Extended Spectrum Beta-Lactamase Escherichia coli in River Waters Collected from Two Cities in Ghana, 2018–2020

et al. 2021

View full text Add to dashboard Cite

Infections by Extended-Spectrum Beta-Lactamase producing Escherichia coli (ESBL-Ec) are on the increase in Ghana, but the level of environmental contamination with this organism, which may contribute to growing Antimicrobial Resistance (AMR), is unknown. Using the WHO OneHealth Tricycle Protocol, we investigated the contamination of E. coli (Ec) and ESBL-Ec in two rivers in Ghana (Odaw in Accra and Okurudu in Kasoa) that receive effluents from human and animal wastewater hotspots over a 12-month period. Concentrations of Ec, ESBL-Ec and percent ESBL-Ec/Ec were determined per 100 mL sample. Of 96 samples, 94 (98%) were positive for ESBL-Ec. concentrations per 100 mL (MCs100) of ESBL-Ec and %ESBL-Ec from both rivers were 4.2 × 104 (IQR, 3.1 × 103–2.3 × 105) and 2.79 (IQR, 0.96–6.03), respectively. MCs100 were significantly lower in upstream waters: 1.8 × 104 (IQR, 9.0 × 103–3.9 × 104) as compared to downstream waters: 1.9 × 106 (IQR, 3.7 × 105–5.4 × 106). Both human and animal wastewater effluents contributed to the increased contamination downstream. This study revealed high levels of ESBL-Ec in rivers flowing through two cities in Ghana. There is a need to manage the sources of contamination as they may contribute to the acquisition and spread of ESBL-Ec in humans and animals, thereby contributing to AMR.

show abstract

“…Of the 37 articles, 26 (70%) were on AMR, 10 (27%) were on antimicrobials, and 1 (3%) was on both. Almost all the articles on AMR in sewage (25 articles, 93%) used culture-based methods, and nearly half (12 articles, 44%) of the articles were focused on Escherichia coli as the target bacterial species [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. The remaining two articles (7%) reported on the use of consecutive ultrafiltration [ 39 ] and metagenomic analysis [ 40 ] to directly detect ARGs in water.…”

Section: Resultsmentioning

confidence: 99%

Review of Antimicrobial Resistance in Wastewater in Japan: Current Challenges and Future Perspectives

et al. 2022

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) circulates through humans, animals, and the environments, requiring a One Health approach. Recently, urban sewage has increasingly been suggested as a hotspot for AMR even in high-income countries (HICs), where the water sanitation and hygiene infrastructure are well-developed. To understand the current status of AMR in wastewater in a HIC, we reviewed the epidemiological studies on AMR in the sewage environment in Japan from the published literature. Our review showed that a wide variety of clinically important antibiotic-resistant bacteria (ARB), antibiotic resistance genes (ARGs), and antimicrobial residues are present in human wastewater in Japan. Their concentrations are lower than in low- and middle-income countries (LMICs) and are further reduced by sewage treatment plants (STPs) before discharge. Nevertheless, the remaining ARB and ARGs could be an important source of AMR contamination in river water. Furthermore, hospital effluence may be an important reservoir of clinically important ARB. The high concentration of antimicrobial agents commonly prescribed in Japan may contribute to the selection and dissemination of AMR within wastewater. Our review shows the importance of both monitoring for AMR and antimicrobials in human wastewater and efforts to reduce their contamination load in wastewater.

show abstract

The level of antimicrobial resistance of sewage isolates is higher than that of river isolates in different Escherichia coli lineages

Cited by 15 publications

References 47 publications

Prevalence and Diversity of Antibiotic Resistant Escherichia coli From Anthropogenic-Impacted Larut River

Prevalence and Diversity of Antibiotic Resistant Escherichia coli From Anthropogenic-Impacted Larut River

Extended Spectrum Beta-Lactamase Escherichia coli in River Waters Collected from Two Cities in Ghana, 2018–2020

Review of Antimicrobial Resistance in Wastewater in Japan: Current Challenges and Future Perspectives

Contact Info

Product

Resources

About