The leukotoxin operon of Fusobacterium necrophorum is not present in other species of Fusobacterium

Oelke, Alisha; Nagaraja, T. G.; Wilkerson, Melinda J.; Stewart, George C.

doi:10.1016/j.anaerobe.2004.10.003

Cited by 20 publications

(9 citation statements)

References 16 publications

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“…All lesion and healthy tissue biopsy samples were subjected to a species-specific F. necrophorum PCR assay as described originally (49). The primers used in this assay (Table 1) target the lktA gene which appears to be unique to F. necrophorum as it is not present in other Fusobacterium species (50).…”

Section: Genus-and Phylogroup-specific Treponeme Pcr Assaysmentioning

confidence: 99%

High-Level Association of Bovine Digital Dermatitis Treponema spp. with Contagious Ovine Digital Dermatitis Lesions and Presence of Fusobacterium necrophorum and Dichelobacter nodosus

Sullivan¹,

Clegg²,

Angell

et al. 2015

J Clin Microbiol

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Digital dermatitis (DD) is an infectious lameness commonly found in dairy cattle worldwide, and it is known as bovine digital dermatitis (BDD) or papillomatous digital dermatitis (PDD). The disease was first reported in 1980 in the United States (1) and in the late 1980s in the United Kingdom (2). BDD has also been confirmed in beef cattle (3, 4), and over the last 30 years, the disease has been recognized as an important cause of bovine lameness (5).Lameness in cattle and sheep has serious animal welfare and economic implications (6-9). The effects of lameness in cattle include a decrease in milk yield (9, 10) and fertility (8, 11-13) and an increase in rate of culling (12,14). This has been found to be particularly true for cattle suffering from BDD (8, 15, 16) with a recent study of the cost of lameness in the United States estimating that on average, BDD costs $133 per case (17).BDD is now a worldwide problem, and controlling BDD on dairy operations has proven difficult. Moreover, in the last 20 years, sheep in the United Kingdom have been identified with a form of DD termed contagious ovine digital dermatitis (CODD), which is rapidly emerging as a severe infectious foot disease since it was first reported in the United Kingdom in 1997 (18-20). Now, CODD has spread into the Republic of Ireland (20), and it was recently reported in dairy goats in the United Kingdom (21), indicating further cross-species transmission. The contagious nature of DD is also evident by the reports of a manifestation of the disease in a wildlife host, North American elk (Cervus elaphus) from Washington State (22). The reports of DD in previously unaffected species, including U.S. wildlife, suggests a much greater global threat of the disease than previously considered.BDD in cattle manifests in several forms, but most frequently as an ulcerative lesion of the digital skin located immediately above the coronary band between the heel bulbs which results in severe lameness (23). The clinical features of CODD in sheep are slightly different, mainly because the initial lesion site on the sheep foot is different. CODD lesions commence at the coronary band and then run under the hoof horn capsule dorsally and abaxially (24). CODD frequently presents a particularly severe outcome where the whole horn capsule can be lost (18,(25)(26)(27). As a result of the severity of the lesions, sheep can be extremely lame, impacting the welfare of the affected sheep (28). This is concurrent with the lesion pathology identified in elk, described as erosive lesions on

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Section: Genus-and Phylogroup-specific Treponeme Pcr Assaysmentioning

confidence: 99%

High-Level Association of Bovine Digital Dermatitis Treponema spp. with Contagious Ovine Digital Dermatitis Lesions and Presence of Fusobacterium necrophorum and Dichelobacter nodosus

Sullivan¹,

Clegg²,

Angell

et al. 2015

J Clin Microbiol

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“…Similarly, Ludlam et al proposed that the lktA gene was not universal between animal and human strains [45], but this was later opposed by Bennet et al [87]. The leukotoxin operon has not been found in other species of Fusobacterium [88], indicating the importance of this virulence factor in all F. necrophorum infections. The uniqueness of leukotoxin produced by F. necro phorum is illustrated by the high antileukotoxin antibody titer, at least after necrobacillosis infection in animals [89].…”

Section: Pathologymentioning

confidence: 93%

Necrobacillosis in humans

Dahlén¹,

Ebenfelt²

2011

Expert Review of Anti-infective Therapy

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Necrobacillosis, often used synonymously with Lemierre's syndrome, is a form of abscess infection in the peritonsillar area associated with a thrombophlebitis and caused by the strict anaerobic species Fusobacterium necrophorum. The thrombosis formed affects the internal jugular vein, from which the bacteria are seeded out in the bloodstream and cause bacteremia. Septicemia is a common complication with an often fatal outcome. Necrobacillosis is very rare and is referred to as the 'forgotten disease'. It is probably frequently overlooked in clinical practice in its early and milder forms such as tonsillitis (sore throat) and peritonsillar abscess. F. necrophorum frequently participates in these infections and is thus suspected to have an etiological role in Lemierre's syndrome. Similarly, F. necrophorum seems to play an important role in noma (cancrum oris) and this disease is also included in the necrobacillosis complex. Diagnosis of infections of the necrobacillosis complex seeks to disclose F. necrophorum in swab samples or blood culture. The most commonly used therapy is metronidazole in combination with penicillin or amoxicillin. Clindamycin is also an option, especially in cases of penicillin allergy.

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“…This was followed by a final 10-min extension at 72°C. The lktA gene appears to be unique to F. necrophorum, as it is reportedly not present in other Fusobacterium species (Oelke et al 2005). The partial DNA sequence of its lktA gene bears accession number AF_312861.…”

Section: Detection Of F Necrophorum and D Nodosusmentioning

confidence: 96%

Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

et al. 2019

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Cow footrot in the Heilongjiang Province, northeast China is a problem resulting in lost production in agriculture. In this study, 200 swab samples from footrot lesions of naturally infected cows with odorous exudative inflammation and keratinous hoof separation at 10 farms were examined in the period from May 2016 to May 2017. Twenty cows from each farm were taken for sampling. The samples were examined for detectingthe presence of Dichelobacter nodosus (D. nodosus) and Fusobacterium necrophorum (F. necrophorum). Such detection was carried out using polymerase chain reaction (PCR). The PCR primers were designed to identify the lktA gene, which encodes a leukotoxin unique to F. necrophorum, and the fimA gene of D. nodosus. Of the 200 samples, 111 (55.5%) revealed the presence of F. necrophorum and 11 (5.5%) exhibited D. nodosus. The frequent finding of F. necrophorum in cow farms of Heilongjiang province, northeast China is noteworthy. The possibility of F. necrophorum and D. nodosus infection should be an important concern when controlling cow footrot in China.

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The leukotoxin operon of Fusobacterium necrophorum is not present in other species of Fusobacterium

Cited by 20 publications

References 16 publications

High-Level Association of Bovine Digital Dermatitis Treponema spp. with Contagious Ovine Digital Dermatitis Lesions and Presence of Fusobacterium necrophorum and Dichelobacter nodosus

High-Level Association of Bovine Digital Dermatitis Treponema spp. with Contagious Ovine Digital Dermatitis Lesions and Presence of Fusobacterium necrophorum and Dichelobacter nodosus

Necrobacillosis in humans

Detection of Fusobacterium necrophorum and Dichelobacter nodosus from cow footrot in the Heilongjiang Province, China

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