The leucocytes during pregnancy

Andrews, William C.; Bonsnes, Roy W.

doi:10.1016/0002-9378(51)90315-8

Cited by 42 publications

(19 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistently, previous studies on pregnancy-associated lymphopenia by flow cytometry have shown that T-and B-cell levels decline with the progression of pregnancy (43)(44)(45) and that recovery of peripheral B-cell levels may occur later than those for T cells (44). Meanwhile, the signature of monocytes showed a more variable pattern, up-regulating in early pregnancy, dipping, and rebounding before delivery, in line with the findings of myeloid immunity activation during pregnancy (43,(46)(47)(48). To further confirm these findings, we reanalyzed another independent maternal plasma RNA dataset by Koh et al (20) collected at three trimesters of pregnancy and within 6-wk postpartum.…”

Section: Noninvasive Elucidation Of Placental Cellular Dynamics Durinsupporting

confidence: 62%

Integrative single-cell and cell-free plasma RNA transcriptomics elucidates placental cellular dynamics

Tsang

Vong

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

257

267

View full text Add to dashboard Cite

The human placenta is a dynamic and heterogeneous organ critical in the establishment of the fetomaternal interface and the maintenance of gestational well-being. It is also the major source of cell-free fetal nucleic acids in the maternal circulation. Placental dysfunction contributes to significant complications, such as preeclampsia, a potentially lethal hypertensive disorder during pregnancy. Previous studies have identified significant changes in the expression profiles of preeclamptic placentas using whole-tissue analysis. Moreover, studies have shown increased levels of targeted RNA transcripts, overall and placental contributions in maternal cell-free nucleic acids during pregnancy progression and gestational complications, but it remains infeasible to noninvasively delineate placental cellular dynamics and dysfunction at the cellular level using maternal cell-free nucleic acid analysis. In this study, we addressed this issue by first dissecting the cellular heterogeneity of the human placenta and defined individual cell-type-specific gene signatures by analyzing more than 24,000 nonmarker selected cells from full-term and early preeclamptic placentas using large-scale microfluidic single-cell transcriptomic technology. Our dataset identified diverse cellular subtypes in the human placenta and enabled reconstruction of the trophoblast differentiation trajectory. Through integrative analysis with maternal plasma cell-free RNA, we resolved the longitudinal cellular dynamics of hematopoietic and placental cells in pregnancy progression. Furthermore, we were able to noninvasively uncover the cellular dysfunction of extravillous trophoblasts in early preeclamptic placentas. Our work showed the potential of integrating transcriptomic information derived from single cells into the interpretation of cell-free plasma RNA, enabling the noninvasive elucidation of cellular dynamics in complex pathological conditions. single-cell transcriptomics | cell-free RNA | noninvasive prenatal testing | placenta | preeclampsia

show abstract

Section: Noninvasive Elucidation Of Placental Cellular Dynamics Durinsupporting

confidence: 62%

Integrative single-cell and cell-free plasma RNA transcriptomics elucidates placental cellular dynamics

Tsang

Vong

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

257

267

View full text Add to dashboard Cite

show abstract

“…The data obtained in this study, though few in number, further supports our earlier suggestion that poor nutritional status and not the effect of sickle cell trait is responsible for lower PCV in Nigerian pregnant women. 13 Leucocytosis due to neutrophilia in pregnancy is known 14 , and this report shows that it occurs in pregnant Nigerian women irrespective of their genotype. We noticed an increase in total leucocytes particularly neutrophil count in the second trimester in subjects with genotype AA and in the third trimester in subjects with genotype AS.…”

Section: Discussionmentioning

confidence: 76%

“…[14][15][16] We found that the lymphocyte count was lower in pregnancy regardless of the genotype, while women with genotype AA had the lowest count in the second trimester with a significant decline between the first and third trimesters. Eosinophil count dropped significantly during pregnancy, which is similar to previous reports 5,17,18 , while basophil and monocyte counts did not show variation in pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Leucocyte Counts in Pregnant Nigerian Women with Sickle Cell Trait

Awodu

Enosolease

Ubaru

2002

African Journal of Reproductive Health

View full text Add to dashboard Cite

White blood count (WBC) with differential counts and packed cell volume (PCV) were studied in 100 pregnant and 30 non-pregnant control women aged 18-45 years. Eighty of the pregnant women were homozygous HbAA and 20 heterozygous HbAS. The non-pregnant women's PCV, lymphocyte and eosinophils counts were significantly higher (p < 0.005) while their leucocytes neutrophil counts were significantly lower (p < 0.005) compared with HbAA and HbAS pregnant women. However, HbAS pregnant women had higher leucocyte, lymphocyte and eosinophil counts compared with HbAA, but these were not significant. There were no variations in basophil and monocyte counts. HbAA pregnant women had no change in PCV but significant changes occured in leucocyte and neurophil counts with increase in the second trimester with decreasing lymphocyte and eosinophil counts in the second and third trimesters. However, HbAS pregnant women had significant increase in PCV in their first trimester, leucocyte and neutrophil counts in their third trimester but no variation in lymphocyte, eosinophil and basophil and monocyte counts. The increased leucocyte and neutrophil counts in the second http://www.bioline.org.br/request?rh02042 (1 of 7)10/20/2004 11:50:36 AM http://www.bioline.org.br/request?rh02042 trimester in HbAA and third trimester in HbAS may be due to genetic factor and/or oestrogen secretion reaching production peak at different periods of pregnancy. The relative increase in these haematological indices in HbAS women may be a protective mechanism against infection during pregnancy. (Afr J Reprod Health 2002; 6[3]: 112-116) RÉSUMÉNumérations leucocytaires chez les femmes nigérianes enceintes qui ont des traits drépanocytaires. Nous avons étudié le nombre de globules blancs et le formulaire leucocytaire et l'hématocrite chez 100 femmes enceintes ainsi que chez 30 femmes témoins âgées de 18-45 ans, qui n'étaient pas enceintes. 80 femmes parmi les femmes enceintes étaient des homozygotes de type HbAA et 20 étaient des hétérozygotes, de type HbAs. Le formulaire leucocytaire, la numération lymphocytaire et la numération des éosinophiles étaient appréciablement élevés alors que leur numération leucocytaire des neutrophiles était appréciablement peu élevée (p 0, 005) comparée à HbAA et HbAS chez les femmes enceintes. Toutefois, les femmes enceintes de la catégorie HbAS avaient des numérations leucocytaires, lymphocytaires et des neutrophiles plus élevés par rapport à HbAA, mais ceux-ci n'étaient pas significatifs. Il n'y avait pas de variations quant aux numérations des basophiles et aux numérations monocytaires. Il n'y avait pas de modification dans le formulaire leucocytaire chez les femmes enceintes de type HbAA, mais il y a eu des modifications assez importantes au niveau des numérations leucocytaires et au niveau de taux des polynucléaires neutrophiles au fur et à mesure que le second trimestre augmentait que les numérations leucocytaires et des éosinophiles baissaient au cours de second et du troisième semestres. Néanmoins, il y a eu des h...

show abstract

“…Explanations in terms of neonatal haemoconcentration are almost certainly erroneous. The maternal neutrophil count is raised throughout pregnancy (Andrews and Bonsnes, 1951), probably as a result of increased oestrogen excretion (Cruickshank et al, 1970), and the maternal count rises further during labour (Andrews and Bonsnes, 1951) as it does in other forms of strenuous muscular exertion (Garrey and Bryan, 1935). The white cell count of the baby at birth mirrors that of the mother in many respects, the neutrophil count (and the proportion of immature cells) after delivery being highest in babies subjected to prolonged labour and a difficult delivery (Strakova, 1964).…”

Section: Resultsmentioning

confidence: 99%