The lethal myospheroid gene of Drosophila encodes a membrane protein homologous to vertebrate integrin beta subunits.

MacKrell, A J; Blumberg, Bruce; Haynes, Susan R.; Fessler, John H.

doi:10.1073/pnas.85.8.2633

Cited by 209 publications

(103 citation statements)

References 33 publications

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“…The role of integrins in various biological processes has been elucidated either by truncation/mutation of the sequences of the receptor or by interference in its functional (MacKrell et al, 1988). The [31-related RGD-blocking peptides have been shown to perturb mesencephalic neural crest migration (Boucaut et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Wada

Kumar

Liu

et al. 1996

The Journal of Cell Biology

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Abstract. Metanephrogenesis has been a long-standing model to study cell-matrix interactions. A number of adhesion molecules, including matrix receptors (i.e., integrins), are believed to be involved in such interactions. The integrins contain a and ~ subunits and are present in various tissues in different heterodimeric forms. In this study, one of the members of the integrin superfamily, av, was characterized, and its relevance in murine nephrogenesis was investigated. Mouse embryonic renal eDNA libraries were prepared and screened for av, and multiple clones were isolated and sequenced. The deduced amino acid sequence of the et v cDNA clones and hydropathic analysis revealed that it has a typical signal sequence and extracellular, transmembrane, and cytoplasmic domains, with multiple Ca 2÷ binding sites. No A(U)nA mRNA instability motifs were present. Conformational analysis revealed no rigid long-range-ordered structure in murine O~v. The O~v was expressed in the embryonic kidney at day 13 of the gestation, with a transcript size of ~7 kb. Its expression increased progressively during the later gestational stages and in the neonatal period. It was distributed in the epithelial elements of developing nephrons and was absent in the uninduced mesenchyme. In mature metanephroi, the expression was relatively high in the glomeruli and blood vessels, as compared to the tubules. Various heterodimeric associations of av, i.e., with [31, [33, [35, and [36, were observed in metanephric tissues. Inclusion of av-antisense-oligodeoxynucleotide or -antibody in metanephric culture induced dysmorphogenesis of the kidney with reduced population of the nephrons, disorganization of the ureteric bud branches, and reduction of mRNA and protein expressions of etv. The expressions of integrin [33, [35, and 136 were unaltered. These findings suggest that the integrin av is developmentally regulated, has a distinct spatio-temporal expression, and is relevant in the mammalian organogenesis.URING development, the interactions between cell adhesion molecules and extraceUular matrix (ECM) 1 glycoproteins are believed to be essential for the morphogenesis of various organs (Hay, 1991;Humphries et al., 1991;Reichardt and Tomaselli, 1991;Ruoslahti, 1991;Toole, 1991;Hynes, 1992;Juliano and Haskill, 1993;Kramer et al., 1993;Gladson and Cheresh, 1994;Hemler et al., 1994;Quaranta et al., 1994). The maturation of the mammalian metanephros constitutes many of the steps which are prototypic of the events relevant to the development of other organ systems (Ekblom, 1993;Clapp and Abrahamson, 1994). The renal organogenesis ensues after an initial reciprocal induction between the metanephric Address all correspondence to Dr. Y.S. Kanwar, Department of Pathology, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611. Tel.: (312) 503-0084. Fax: (312) 503-0627.1. Abbreviations used in this paper: ECM, extracellular matrix; ODN, oligodeoxynucleotide; RT, reverse transcriptase. mesenchyme and the ureteric bud, which leads to...

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Section: Discussionmentioning

confidence: 99%

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Wada

Kumar

Liu

et al. 1996

The Journal of Cell Biology

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“…2C,D), allowing any enhancement of rotational defects to be easily recognised. Halving the dose of α-laminin [wing blister (Martin et al, 1999)] and the integrin β subunit [myospheroid (MacKrell et al, 1988)] did not modify the Star/+ phenotype. In contrast, alleles of E-cadherin [shotgun (Tepass et al, 1996)] showed a significant interaction with Star, with many more misrotated ommatidia (Fig.…”

Section: Cadherin-based Adhesion Is Involved In Rotationmentioning

confidence: 99%

Egfr signalling defines a protective function for ommatidial orientation in theDrosophilaeye

Brown

Freeman

2003

Development

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Ommatidial rotation in the Drosophila eye provides a striking example of the precision with which tissue patterning can be achieved. Ommatidia in the adult eye are aligned at right angles to the equator, with dorsal and ventral ommatidia pointing in opposite directions. This pattern is established during disc development, when clusters rotate through 90°, a process dependent on planar cell polarity and rotation-specific factors such as Nemo and Scabrous. Here, we demonstrate a requirement for epidermal growth factor receptor (Egfr) signalling in rotation, further adding to the manifold actions of this pathway in eye development. Egfr is distinct from other rotation factors in that the initial process is unaffected, but orientation in the adult is greatly disrupted when signalling is abnormal. We propose that Egfr signalling acts in the third instar imaginal disc to `lock' ommatidia in their final position, and that in its absence, ommatidial orientation becomes disrupted during the remodelling of the larval disc into an adult eye. This lock may be achieved by a change in the adhesive properties of the cells:cadherin-based adhesion is important for ommatidia to remain in their appropriate positions. In addition, we have evidence that there is an error-correction mechanism operating during pupal stages to reposition inappropriately orientated ommatidia. Our results suggest that initial patterning events are not sufficient to achieve the precise architecture of the fly eye, and highlight a novel requirement for error-correction, and for an Egfr-dependent protection function to prevent morphological disruption during tissue remodelling.

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“…(Harden et al 1996;Lotz et al 1997Lotz et al , 2000Stark et al 1997;Goldfinger et al 1999;Mercurio et al 2001). For example, mutations in the Drosophila integrin receptor ␤-subunit (myospheroid) and in the ␣3 subunit (scab) of the integrin receptor lead to a DC defect (MacKrell et al 1988;Stark et al 1997).…”

Section: Dorsal Closure In Drosophila As a Model System For Wound Repairmentioning

confidence: 99%

Role of Rho family GTPases in epithelial morphogenesis

Aelst¹,

Symons²

2002

Genes Dev.

213

184

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The lethal myospheroid gene of Drosophila encodes a membrane protein homologous to vertebrate integrin beta subunits.

Cited by 209 publications

References 33 publications

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Egfr signalling defines a protective function for ommatidial orientation in theDrosophilaeye

Role of Rho family GTPases in epithelial morphogenesis

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