The Leishmania chagasi proteasome: role in promastigotes growth and amastigotes survival within murine macrophages

Silva, Alexandre de Almeida e; Horta, Maria Fátima; Ramalho-Pinto, F. J.

doi:10.1016/j.actatropica.2004.03.007

Cited by 39 publications

(29 citation statements)

References 31 publications

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“…, 2007). In Leishmania parasites, inhibition of the proteasome activity was shown to impair the growth of extracellular promastigotes and the survival of intracellular amastigotes (Robertson, 1999; Silva‐Jardim et al. , 2004), but whether the proteasome contributes to the defence against ROI or RNI is currently unknown.…”

Section: Mechanisms Of Evasion and Remodelling Of The Immune Systemmentioning

confidence: 99%

Mechanisms and consequences of persistence of intracellular pathogens: leishmaniasis as an example

Bogdan¹

2008

Cell Microbiol

131

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SummaryLifelong persistence after clinical cure of the primary infection is a characteristic feature of many intracellular pathogens, including viruses, bacteria and protozoa. The underlying mechanisms are complex and range from the passive protection against toxic effector molecules of the host and the remodelling of intracellular compartments as safe niches to the active modulation of the immune response at multiple levels. Parasites of the genus Leishmania have been particular helpful in unravelling some of the basic processes and form therefore the centre of the discussion.

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Section: Mechanisms Of Evasion and Remodelling Of The Immune Systemmentioning

confidence: 99%

Mechanisms and consequences of persistence of intracellular pathogens: leishmaniasis as an example

Bogdan¹

2008

Cell Microbiol

131

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“…Surviving cells were shorter with rounded apical ends and had DNA and mitochondrial damage. After 6 days of treatment of Leishmania chagasi with 50 μ m lactacystin, a 10‐fold reduction in viable cells was recorded; while exposure of promastigotes to lactacystin prior to infection of macrophages decreased survival in the host cell by 98% . Proteins essential to cell cycle were identified as proteasome substrates in these parasites .…”

Section: Protozoan Parasitesmentioning

confidence: 99%

Targeting proteasomes in infectious organisms to combat disease

et al. 2017

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Proteasomes are multisubunit, energy-dependent, proteolytic complexes that play an essential role in intracellular protein turnover. They are present in eukaryotes, archaea, and in some actinobacteria species. Inhibition of proteasome activity has emerged as a powerful strategy for anticancer therapy and three drugs have been approved for treatment of multiple myeloma. These compounds react covalently with a threonine residue located in the active site of a proteasome subunit to block protein degradation. Proteasomes in pathogenic organisms such as Mycobacterium tuberculosis and Plasmodium falciparum also have a nucleophilic threonine residue in the proteasome active site and are therefore sensitive to these anticancer drugs. This review summarizes efforts to validate the proteasome in pathogenic organisms as a therapeutic target. We describe several strategies that have been used to develop inhibitors with increased potency and selectivity for the pathogen proteasome relative to the human proteasome. In addition, we highlight a cell-based chemical screening approach that identified a potent, allosteric inhibitor of proteasomes found in Leishmania and Trypanosoma species. Finally, we discuss the development of proteasome inhibitors as anti-infective agents.

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“…The methodology employed to obtain mice peritoneal macrophages was described [16,22]. BALB/c mice were i.p.…”

Section: In Vitro Infection and Treatments Of Peritoneal Macrophagesmentioning

confidence: 99%

Liposomes containing an ASP49-phospholipase A 2 from Bothrops jararacussu snake venom as experimental therapy against cutaneous leishmaniasis

Barros

Macedo

Ferreira

et al. 2016

International Immunopharmacology

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The Leishmania chagasi proteasome: role in promastigotes growth and amastigotes survival within murine macrophages

Cited by 39 publications

References 31 publications

Mechanisms and consequences of persistence of intracellular pathogens: leishmaniasis as an example

Mechanisms and consequences of persistence of intracellular pathogens: leishmaniasis as an example

Targeting proteasomes in infectious organisms to combat disease

Liposomes containing an ASP49-phospholipase A 2 from Bothrops jararacussu snake venom as experimental therapy against cutaneous leishmaniasis

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