The Legume Nodule Microbiome: A Source of Plant Growth-Promoting Bacteria

Velázquez, Encarna; Carro, Lorena; Flores-Félix, José David; Martínez‐Hidalgo, Pilar; Menéndez, Esther; Ramírez-Bahena, Martha-Helena; Mulas, Rebeca; González‐Andrés, Fernando; Martínez‐Molina, Eustoquio; Peix, Álvaro

doi:10.1007/978-981-10-3473-2_3

Cited by 27 publications

(26 citation statements)

References 223 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of non‐nodulating bacteria in the nodule interior is now well‐documented though their role is not yet understood (Martínez‐Hidalgo and Hirsch ; Velázquez et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…Lupinus species have been shown to be nodulated by slow-growing rhizobia of the genus Bradyrhizobium, including B. japonicum, B. lupini, B. canariense, B. diazoefficiens and B. elkanii species (Peix et al 2015;Shamseldin et al 2017;Vel azquez et al 2017;Ste z pkowski et al 2018;Mellal et al 2019). Also, fast-growing strains of the species Ochrobactrum lupini isolated from Lotus honoratus (Trujillo et al 2005), of the genus Microvirga from L. texensis (Ardley et al 2012), L. micranthus (Msaddak et al 2017a(Msaddak et al , 2017b and L. subcarnosus (Beligala et al 2017) and Burkholderia have been isolated from the later plant species.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

et al. 2019

View full text Add to dashboard Cite

Aim To analyse the diversity of nodule‐forming bacteria isolated from Lupinus cosentinii naturally grown in the Maamora cork oak forest (Rabat, Morocco). Methods and Results Of the 31 bacterial strains, four were selected based on their REP‐PCR fingerprinting that were studied by sequencing and phylogenetic analysis of their 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes as well as the nodC symbiotic gene. The nearly complete 16S rRNA gene sequence of the four representative strains showed that they are related to Tunisian strains of genus Microvirga isolated from L. micranthus with nucleotide identity values ranging from 98·67 to 97·13%. The single and concatenated sequences of the 16S rRNA, gyrB, dnaK, recA and rpoB housekeeping genes indicated that the L. cosentinii‐isolated strains had 99·2–99·9% similarities with the Tunisian L. micranthus microsymbionts. The nodC gene phylogeny revealed that the Moroccan strains clustered in the newly described mediterranense symbiovar, and nodulation tests showed that they nodulated not only L. cosentinii but also L. angustifolius, L. luteus and L. albus. Conclusions To the best of our knowledge, this is the first report concerning the isolation, molecular identification and phylogenetic diversity of L. cosentinii nodule‐forming endosymbionts and of their description as members of the Microvirga genus. Significance and Impact of the Study In this work, we show that Microvirga sp. can be isolated from root nodules of wild‐grown L. cosentinii in Northeast Africa, that selected strains also nodulate L. angustifolius, L. luteus and L. albus, and that they belong to symbiovar mediterranense. In addition, our data support that the ability of Microvirga to nodulate lupines could be related to the soil pH, its geographical distribution being more widespread than expected.

show abstract

“…The presence of non‐nodulating bacteria in the nodule interior is now well‐documented though their role is not yet understood (Martínez‐Hidalgo and Hirsch ; Velázquez et al . ).…”

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Among these processes, we emphasize biological nitrogen fixation (BNF), carried out by symbiotic and associative diazotrophic bacteria, which is extremely important for the ecosystems by making nitrogen available in a form that can be assimilated by plants, which promotes greater plant development. Nevertheless, promotion of plant growth can be achieved through other processes, such as phytohormone production (Costa et al, 2016), phosphate solubilization (Angus et al, 2013;Costa et al, 2016), siderophore production (Wdowiak-Wróbel et al, 2017), and adaptation to stresses, such as tolerance to metals (Rangel et al, 2016), in leguminous and non-leguminous plants (Velázquez et al, 2017).…”

mentioning

confidence: 99%

Plant growth‐promoting rhizobacterial communities from an area under the influence of iron mining and from the adjacent phytophysiognomies which have high genetic diversity

et al. 2020

View full text Add to dashboard Cite

We evaluated symbiotic (rhizobia) and non-symbiotic efficiency and the diversity of native communities of plant growth-promoting rhizobacteria (PGPR) in soil samples from an area in recovery after iron mining (RA) and from adjacent phytophysiognomies (Ironstone Outcrops, Neotropical Savanna, Atlantic Forest) in the Quadrilátero Ferrífero region, MG, Brazil, in two climate seasons. Greenhouse experiments were conducted with siratro (Macroptilium atropurpureum) as a trap plant. The SPAD index in siratro was more effective in detecting the difference between the symbiotic efficiencies of the communities of PGPR than shoot dry matter, with readings of the SPAD index greater in RA. Genetic diversity, of 327 strains evaluated by partial sequencing of the 16S rRNA gene, showed 29 genera in the dry season and 19 in the rainy season, among these genera some recognized as nodulating and endophytes, and others potentially endophytes. Genera belonging to the phyla Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were found. The nodule-forming genera Bradyrhizobium and Rhizobium and the endophytic Paenibacillus were those of highest occurrence in both seasons and in all phytophysiognomies. The greater symbiotic efficiency and the high genetic diversity of the rhizobacterial community found in RA indicate that soil rehabilitation is effectively in process. As most of native legume species form symbiosis with Rhizobium and mainly Bradyrhizobium, these bacterial communities are an important source of potential inoculant strains, as well as, a relevant feature for the successful establishment of diverse legumes for revegetation purposes.

show abstract

“…In this work, have recurrently mentioned the microbiome from legume root nodules, because besides accommodating rhizobia strains, these nodules can also be occupied by diverse non-rhizobial bacteria [38][39][40]. Interestingly, there have been some works proposing the microbiomes of legume nodules to be key sources of endophytic PPBs (RBEs and NRBEs), claiming that this particular legume organ harbors a wide range of beneficial endophytes with plant growth promotion features that fulfil biosafety rules [40][41][42]. Rhizobia are a complex group of Gram-negative bacteria that live in the soil as saprophytes or in association with plants, as endophytes of inner plant tissues.…”

Section: The Plant Microbiome With a Focus On The Roots And Endophytmentioning

confidence: 99%

“…Restored production with the combination 50% fertilizer + consortium. [103] In the particular case of legumes, RBEs and NRBEs living within nodules certainly act synergistically to increase plant growth, health, and survival [40,41]. Interestingly, some studies show that the taxonomic composition of the nodule microbiome varies depending on which rhizobium has induced the nodules.…”

Section: Better Together-inoculation With Ppb Consortia Vs Individuamentioning

confidence: 99%

Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops

Menéndez

Paço

2020

Life

Self Cite

View full text Add to dashboard Cite

The overgrowth of human population and the demand for high-quality foods necessitate the search for sustainable alternatives to increase crop production. The use of biofertilizers, mostly based on plant probiotic bacteria (PPB), represents a reliable and eco-friendly solution. This heterogeneous group of bacteria possesses many features with positive effects on plants; however, how these bacteria with each other and with the environment when released into a field has still barely been studied. In this review, we focused on the diversity of root endophytic rhizobial and non-rhizobial bacteria existing within plant root tissues, and also on their potential applications as consortia exerting benefits for plants and the environment. We demonstrated the benefits of using bacterial inoculant consortia instead of single-strain inoculants. We then critically discussed several considerations that farmers, companies, governments, and the scientific community should take into account when a biofertilizer based on those PPBs is proposed, including (i) a proper taxonomic identification, (ii) the characterization of the beneficial features of PPB strains, and (iii) the ecological impacts on plants, environment, and plant/soil microbiomes. Overall, the success of a PPB consortium depends on many factors that must be considered and analyzed before its application as a biofertilizer in an agricultural system.

show abstract

The Legume Nodule Microbiome: A Source of Plant Growth-Promoting Bacteria

Cited by 27 publications

References 223 publications

Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

Microvirgasp. symbiovar mediterranense nodulatesLupinus cosentiniigrown wild in Morocco

Plant growth‐promoting rhizobacterial communities from an area under the influence of iron mining and from the adjacent phytophysiognomies which have high genetic diversity

Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops

Contact Info

Product

Resources

About