The legacy pesticide dieldrin acts as a teratogen and alters the expression of dopamine transporter and dopamine receptor 2a in zebrafish (Danio rerio) embryos

Sarty, Kathleena I.; Cowie, Andrew; Martyniuk, Christopher J.

doi:10.1016/j.cbpc.2017.01.010

Cited by 13 publications

(11 citation statements)

References 71 publications

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“…Two D 1 ‐like receptors were measured ( drd1b and drd7 , which is also called drd1e ) (Figures and S2A). We did not measure drd1a in this study and sequencing of the amplicon suggests that we are measuring the drd1b paralog in our study. Expression levels significantly varied over the 72‐hour time course for drd1b ( K = 36.3; P < .0001).…”

Section: Resultsmentioning

confidence: 88%

“…Two D 1 -like receptors were measured (drd1b and drd7, which is also called drd1e) (Figures 1 and S2A). We did not measure drd1a in this study and sequencing of the amplicon 21 Figure S3F) and was highest in expression at 72 hpf.…”

Section: Discussionmentioning

confidence: 92%

“…With DA playing an integral role in vertebrate cognition and behavior, chemicals that interact with this system are of high interest. Dieldrin, a neuroactive pesticide, decreased the transcript abundance of slc6a3 and drd2a in dechorionated ZF embryos at 34.7 μM . Sarty et al suggested that downregulation of drd2a may inhibit DA signaling, and this was hypothesized to be related to the lethargy exhibited by ZF larvae following exposure to the pesticide.…”

Section: Discussionmentioning

confidence: 99%

“…Dieldrin, a neuroactive pesticide, decreased the transcript abundance of slc6a3 and drd2a in dechorionated ZF embryos at 34.7 μM . Sarty et al suggested that downregulation of drd2a may inhibit DA signaling, and this was hypothesized to be related to the lethargy exhibited by ZF larvae following exposure to the pesticide. Deltamethrin, a commonly used pyrethroid insecticide, reduced drd1 mRNA in 72 hpf ZF at 1 and 1.5 nM, while increasing th1 mRNA transcript levels at 0.75 nM .…”

Section: Discussionmentioning

confidence: 99%

“…Primer sets for target genes were collected from literature or were newly designed (Table S1, Supporting Information). Primers were designed by Primer‐BLAST (NCBI) .…”

Section: Methodsmentioning

confidence: 99%

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Domperidone upregulates dopamine receptor expression and stimulates locomotor activity in larval zebrafish (Danio rerio)

Shontz

Souders

Schmidt

et al. 2018

Genes Brain and Behavior

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Dopamine (DA) plays a significant role in cognition, motor function and social behavior. The objectives of this study were to (1) quantify the temporal expression of transcripts (DA receptors, transporters and tyrosine hydroxylase) associated with DA signaling during early stages of zebrafish development and (2) determine their expression profiles following treatment with a D receptor antagonist domperidone (DMP). We also assessed locomotor behavior following treatment with DMP using alternating periods of light and dark (ie, dark photokinesis), as DA plays a key role in behavior. Relative expression levels of transcripts that were investigated and related to the DA system were detected after the first 24 hours postfertilization (hpf). Some DA receptor transcripts (eg, drd4c) increased in abundance earlier in the embryo compared with other receptors (eg, drd3), suggesting that DA receptor paralogs may have unique roles in development. Treatment of larvae with DMP resulted in the upregulation of DA receptor transcripts (ie, drd1, drd7, drd4b, drd4c) and DA transporter 1 (ie, slc6a3), and it is hypothesized that upregulation of genes related to the DA system is a compensatory neurophysiological response to DA receptor antagonism. Larval activity during dark photokinesis (measured by distance traveled) was also elevated by DMP. We hypothesize that behavioral responses observed with DMP may be related to the regulation of deep brain photoreception in zebrafish (Danio rerio) (ZF) larvae by DA.

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Section: Resultsmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Primer sets for target genes were collected from literature or were newly designed (Table S1, Supporting Information). Primers were designed by Primer‐BLAST (NCBI) .…”

Section: Methodsmentioning

confidence: 99%

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