The layered, asymmetric structure of the plasma membrane in the exocrine pancreas cells of the cat

Sjöstrand, Fritiof S.; Elfvin, L.-G.

doi:10.1016/s0022-5320(62)90043-6

Cited by 65 publications

(7 citation statements)

References 17 publications

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“…Isolated prostasomes were fixed for 6 hr in 2% glutaraldehyde in 0.1 mom cacodylate buffer, pH 7.2, supplemented with 0.1 moVL sucrose (cac-buffer), postfixed in 1% osmium tetroxide in the cac-buffer for 1M hr, dehydrated in ethanol, and embedded in the epoxy resin Agar 100 (Agar Scientific Ltd., Standsted, Essex, England). Ultrathin sections (50 nm) were placed on copper grids covered with a f i l m of polyvinyl formal plastic (Formvar, Agar Scientific Ltd.) and contrastated with uranyl acetate and lead citrate [23,24]. The diameters of more than 400 membranesurrounded prostasomes were measured in each section taken from three different levels in pellets from two separate experiments.…”

Section: Electron Microscopic Morphologymentioning

confidence: 99%

“…Briefly, after blocking of unspecific binding with normal goat serum, type V (Sigma Biochemicals, St. Louis, MO) the sections were incubated with the primary antibodies (see below) for 2 hr at 20°C or for 16 hr at 4°C and with the secondary antibodies GAR-G5 and G15 or GAM-G5 and G15 (Amersham International, Buckinghamshire, England), diluted 1/20 for 2 hr at 20°C. Finally, the sections were contrasted with 4% uranyl acetate [23] and lead citrate [24]. The 0.05-mom Trisbuffered saline (TBS), pH 7.2, with 0.1% BSA (Sigma Biochemicals) was used for dilution of the primary antibodies, and TBS, pH 8.2, with 1% BSA for the secondary antibodies.…”

Section: Electron M Icroxop Ic I Mmunocytochem Istrymentioning

confidence: 99%

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Prostasomes are neuroendocrine-like vesicles in human semen

et al. 1996

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BACKGROUND Prostasomes are prostate‐derived organelles that exist extracellularly in human seminal plasma. METHODS In this study, we have investigated and characterized human prostasomes with regard to their contents of synaptophysin, members of the chromogranin family, and some neuropeptides. RESULTS By radioimmunoassay measurement and electron microscopy we show the presence of the neuroendocrine markers chromogranin B, neuropeptide Y, and vasoactive intestinal polypeptide in about equimolar amount in human prostasomes and chromogranin A in about 2% of that amount. To our knowledge, such a high ratio of chromogranin B to chromogranin A has never before been observed. The membrane‐bound protein synaptophysin, a well‐established immunocytochemical marker for neuroendocrine cells and neurones, was also detected. Hence, we show that synaptophysin could be used as a marker for intact prestasomes. CONCLUSIONS The presence of synaptophysin has recently been shown in the serotonin‐containing vesicles in platelets. A protein with a similar structure denoted granulophysin has been found in granulocytes and prostasomes. It is suggested that synaptophysin and granulophysin molecules are members of a family of proteins, maybe expressed in all cells that have regulated release of granule content. Our presented data indicate a neurotransmittor function of the prostasomes. The target cells are however not known but could be either the spermatozoa, the epithelial mucous cells of the uterus or tubas or perhaps the ovum. © 1996 Wiley‐Liss, Inc.

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Section: Electron Microscopic Morphologymentioning

confidence: 99%

Section: Electron M Icroxop Ic I Mmunocytochem Istrymentioning

confidence: 99%

Prostasomes are neuroendocrine-like vesicles in human semen

et al. 1996

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“…144-150), some of the relevant components of capillary endothelial cells will be reviewed (14,15). The membranes of these cells follow in principle those of other cells (16)(17)(18)(19)(20)(21)(22)(23). The membranes are a phospholipid double layer, which is electrically relatively insulating.…”

Section: Construction Of the Vicc Systemmentioning

confidence: 99%

An additional circulatory system: Vascular-Interstitial closed electric circuits (VICC)

Nordenström

1987

J Biol Phys

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Abstract:The construction and activation of a previously described additional circulatory system for long-distance transport in tissue is summarized. Metabolism or injury produces electrochemical polarization of tissue. This driving force produces exchange of material by electrophoresis and dielectrophoresis in tissue and cellular matrices with fixed surface charges. These transports are described over vascular-intersititial closed electric circuits (VICC) because blood vessels function as relatively insulated conducting cables and connect with the conducting tissue fluids over capillaries. Necessary electron tranfer takes place at sites of segmental capillary contractions by the superimposed electric field via globular proteins known to exchange electrons in the endothelial cellular membranes. The effect of field-activated VICC is here demonstrated by accumulation of granulocytes in vivo as an electrophoretic process, which gives a mechanistic and energetic logical explanation of so-called leukotaxis.

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“…The present author described in the previous study (ICHIKAWA, 1965) (SJOSTRAND et al, 1962) of MILLONIG's solution. For convenience sake of description, these three fixation methods will be called immersion, dripping and injection methods, respectively, in the following manuscript.…”

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confidence: 94%

A Note on the “Dark” Cells of the Exocrine Pancreas

Ichikawa

1967

Arch. Histol. Jap., Arch. Histol. Jpn

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The layered, asymmetric structure of the plasma membrane in the exocrine pancreas cells of the cat

Cited by 65 publications

References 17 publications

Prostasomes are neuroendocrine-like vesicles in human semen

Prostasomes are neuroendocrine-like vesicles in human semen

An additional circulatory system: Vascular-Interstitial closed electric circuits (VICC)

A Note on the “Dark” Cells of the Exocrine Pancreas

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