The launch of satellite: DNA repeats as a cytogenetic tool in discovering the chromosomal universe of wild Triticeae

Kroupin, Pavel Yu.; Ulyanov, Daniil S.; Карлов, Г. И.; Divashuk, Mikhail G.

doi:10.1007/s00412-023-00789-4

Cited by 6 publications

(5 citation statements)

References 236 publications

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“…Overall, 5S rDNA occurred in at least two chromosomes in all 64 plants. With advances in science and technology, the occurrence of 5S rDNA has been confirmed in an increasing amount of species (de Barros et al 2023, de Moraes et al 2023, Kroupin et al 2023, Rodríguez-González et al 2023). Whether the reported length of 5S rDNA is a complete or partial sequence, there is no doubt there is a big difference among these 5S rDNA, including the length and base pair (Röser et al 2001, Kulak et al 2002, Moraes et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 21 species

Luo,

Liu,

Gong

et al. 2024

Preprint

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5S rDNA is essential component of all cell types. A survey was conducted to study the 5S rDNA site number, position, and origin of signal pattern diversity in 64 plants using fluorescencein situhybridization. The species used for this experiment were chosen due to the discovery of karyotype rearrangement, and for species checked in which 5S rDNA has not yet been explored. The chromosome number was 14160, while the chromosome length was 0.636.88 m, with 37 plants (58%) had small chromosome (<3 m). The chromosome numbers of three species and 5S rDNA loci of 19 species have been reported for the first time. 5S rDNA was varied and abundant in signal site number (218), position (e.g., interstitial, distal, and proximal position, occasionally, outside chromosome), and even as intense (e.g., strong, weak, and slight). Our results exposed modifiability in the number and location of 5S rDNA in 33 plants and demonstrated an extensive stable number and location of 5S rDNA in 31 plants. The potential origin of signal pattern diversity was probably caused by chromosome rearrangement (e.g., deletion, duplication, inversion, translocation), polyploidization, self-incompatibility, and chromosome satellites. These data characterized the variability of 5S rDNA within the karyotypes of the 64 plants that exhibited massive chromosomal rearrangements and provided anchor points for genetic physical maps. These data prove the utility of the 5S rDNA oligonucleotide as a chromosome marker in identifying plant chromosomes. This method provides a basis for developing similar applications for cytogenetic analysis in other species.

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Section: Discussionmentioning

confidence: 99%

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 21 species

Luo,

Liu,

Gong

et al. 2024

Preprint

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“…There are approximately 83–92% repeated sequences in the genome of Triticeae species [ 60 , 61 ], and these sequences have a strong linear correspondence on the chromosomes of different species within the family, which allows oligo-probes designed from repeated sequences to be used jointly between related species [ 62 , 63 ]. Therefore, oligo-pSc119.2 and pTa535 used in the identification of chromosome recombination in D88-2a may also be successfully hybridized on the chromosomes of rye (RR), Leymus mollis (NsNsXmXm), Agropyron Gaertn (PP) and Thinopyrum elongatum (EE) [ 34 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

The addition of Psathyrostachys Huashanica Keng 6Ns large segment chromosomes has positive impact on stripe rust resistance and plant spikelet number of common wheat

Li,

Cheng

et al. 2024

BMC Plant Biol

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Background Developing novel germplasm by using wheat wild related species is an effective way to rebuild the wheat resource bank. The Psathyrostachys huashanica Keng (P. huashanica, 2n = 2x = 14, NsNs) is regarded as a superior species to improve wheat breeding because of its multi-resistance, early maturation and numerous tiller traits. Introducing genetic components of P. huashanica into the common wheat background is the most important step in achieving the effective use. Therefore, the cytogenetic characterization and influence of the introgressed P. huashanica large segment chromosomes in the wheat background is necessary to be explored. Results In this study, we characterized a novel derived line, named D88-2a, a progeny of the former characterized wheat-P. huashanica partial amphiploid line H8911 (2n = 7x = 49, AABBDDNs). Cytological identification showed that the chromosomal composition of D88-2a was 2n = 44 = 22II, indicating the addition of exogenous chromosomes. Genomic in situ hybridization demonstrated that the supernumerary chromosomes were a pair of homologues from the P. huashanica and could be stably inherited in the common wheat background. Molecular markers and 15 K SNP array indicated that the additional chromosomes were derived from the sixth homoeologous group (i.e., 6Ns) of P. huashanica. Based on the distribution of the heterozygous single-nucleotide polymorphism sites and fluorescence in situ hybridization karyotype of each chromosome, this pair of additional chromosomes was confirmed as P. huashanica 6Ns large segment chromosomes, which contained the entire short arm and the proximal centromere portion of the long arm. In terms of the agronomic traits, the addition line D88-2a exhibited enhanced stripe rust resistance, improved spike characteristics and increased protein content than its wheat parent line 7182. Conclusions The new wheat germplasm D88-2a is a novel cytogenetically stable wheat-P. huashanica 6Ns large segment addition line, and the introgressed large segment alien chromosome has positive impact on plant spikelet number and stripe rust resistance. Thus, this germplasm can be used for genetic improvement of cultivated wheat and the study of functional alien chromosome segment.

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“…crassa [ 51 ] ( Table 3 ). The Afa family is commonly used for chromosome identification in the Triticeae tribe and typically results in the detection of multiple subtelomeric, proximal, and interstitial hybridization sites on chromosomes [ 44 , 51 , 73 , 80 ]. CL82, CL89, and CL192 showed only pericentromeric signals in Ps.…”

Section: Discussionmentioning

confidence: 99%

“…They are widely used for karyotyping chromosomes, studying chromosomal rearrangements, and analyzing the genomic composition of allo- and autopolyploids and wide hybrids using fluorescence in situ hybridization (FISH) [ 38 , 39 , 40 , 41 , 42 ]. Comparative characteristics between Triticeae species can be studied by comparing the copy numbers of repeating elements [ 43 ], by comparing the distribution patterns across chromosomes and genomes [ 44 , 45 , 46 , 47 ], or by using a combination of both approaches [ 48 , 49 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Pseudoroegneria libanotica and Pseudoroegneria tauri Based on Their Repeatome Peculiarities

Kroupin,

Yurkina,

Ulyanov

et al. 2023

Plants

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Pseudoroegneria species play an important role among Triticeae grasses, as they are the putative donors of the St genome in many polyploid species. Satellite repeats are widely used as a reliable tool for tracking evolutionary changes because they are distributed throughout the genomes of plants. The aim of our work is to perform a comparative characterization of the repeatomes of the closely related species Ps. libanotica and Ps. tauri, and Ps. spicata was also included in the analysis. The overall repeatome structures of Ps. libanotica, Ps. tauri, and Ps. spicata were similar, with some individual peculiarities observed in the abundance of the SIRE (Ty1/Copia) retrotransposons, Mutator and Harbinger transposons, and satellites. Nine new satellite repeats that have been identified from the whole-genome sequences of Ps. spicata and Ps. tauri, as well as the CL244 repeat that was previously found in Aegilops crassa, were localized to the chromosomes of Ps. libanotica and Ps. tauri. Four satellite repeats (CL69, CL101, CL119, CL244) demonstrated terminal and/or distal localization, while six repeats (CL82, CL89, CL168, CL185, CL192, CL207) were pericentromeric. Based on the obtained results, it can be assumed that Ps. libanotica and Ps. tauri are closely related species, although they have individual peculiarities in their repeatome structures and patterns of satellite repeat localization on chromosomes. The evolutionary fate of the identified satellite repeats and their related sequences, as well as their distribution on the chromosomes of Triticeae species, are discussed. The newly developed St genome chromosome markers developed in the present research can be useful in population studies of Ps. libanotica and Ps. tauri; auto- and allopolyploids that contain the St genome, such as Thinopyrum, Elymus, Kengyilia, and Roegneria; and wide hybrids between wheat and related wild species.

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The launch of satellite: DNA repeats as a cytogenetic tool in discovering the chromosomal universe of wild Triticeae

Cited by 6 publications

References 236 publications

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 21 species

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 21 species

The addition of Psathyrostachys Huashanica Keng 6Ns large segment chromosomes has positive impact on stripe rust resistance and plant spikelet number of common wheat

Comparative Characterization of Pseudoroegneria libanotica and Pseudoroegneria tauri Based on Their Repeatome Peculiarities

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