The latex of Euphorbia tirucalli inhibits staphyloxanthin production and protects Tenebrio molitor larvae against Staphylococcus aureus infection

Colasso, Arthur Henrique Mendes; Barros, Thayná Fernandes; Figueiredo, Isabella Fernandes da Silva; Carvalho, Alexsander Rodrigues; Fernandes, Elizabeth S.; Uchoa, Michelle Russo Bendelak; Silva, Luís Cláudio Nascimento da

doi:10.1080/14786419.2019.1582036

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…Other antivirulence effect of EbEO is the inhibition of STX production, a carotenoid from S. aureus membrane that confers protection against reactive oxygen species produced by host defense, allowing the bacteria to persist in the inflammation site (Liu and Nizet, 2009;Pannu et al, 2019). These properties make STX an attractive target for antivirulence therapy as demonstrated for some plant-derived compounds (Lee et al, 2013;Silva et al, 2017;Colasso et al, 2019). The inhibitory effects of EbEO toward STX production could be confirmed by the fact that S. aureus grown in the presence of this oil showed increased susceptibility to hydrogen peroxide.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

Filho

Silva

et al. 2020

Front. Microbiol.

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Eugenia brejoensis L. (Myrtaceae) is an endemic plant from caatinga ecosystem (brazilian semi-arid) which have an E. brejoensis essential oil (EbEO) with reported antimicrobial activity. In this work, in vitro and in vivo models were used to characterize the inhibitory effects of EbEO in relation to Staphylococcus aureus. EbEO inhibited the growth of all tested S. aureus strains (including multidrug resistance isolates) with values ranging from 8 to 516 µg/mL. EbEO also synergistically increased the action of ampicillim, chloramphenicol, and kanamycin. The treatment with subinhibitory concentrations (Sub-MIC) of EbEO decreased S. aureus hemolytic activity and its ability to survive in human blood. EbEO strongly reduced the levels of staphyloxanthin (STX), an effect related to increased susceptibility of S. aureus to hydrogen peroxide. The efficacy of EbEO against S. aureus was further demonstrated using Caenorhabditis elegans and Galleria mellonella. EbEO increased the lifespan of both organisms infected by S. aureus, reducing the bacterial load. In addition, EbEO reduced the severity of S. aureus infection in G. mellonella, as shown by lower levels of melanin production in those larvae. In summary, our data suggest that EbEO is a potential source of lead molecules for development of new therapeutic alternatives against S. aureus.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

Filho

Silva

et al. 2020

Front. Microbiol.

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“…The damage caused by natural products used in T. molitor larvae was also evaluated. The degree of suffering was observed through melanization, movement, reaction to stimuli, and survival [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Chemical Characterization and Leishmanicidal Activity In Vitro and In Silico of Natural Products Obtained from Leaves of Vernonanthura brasiliana (L.) H. Rob (Asteraceae)

et al. 2023

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Vernonanthura brasiliana (L.) H. Rob is a medicinal plant used for the treatment of several infections. This study aimed to evaluate the antileishmanial activity of V. brasiliana leaves using in vitro and in silico approaches. The chemical composition of V. brasiliana leaf extract was determined through liquid chromatography-mass spectrometry (LC-MS). The inhibitory activity against Leishmania amazonensis promastigote was evaluated by the MTT method. In silico analysis was performed using Lanosterol 14alpha-demethylase (CYP51) as the target. The toxicity analysis was performed in RAW 264.7 cells and Tenebrio molitor larvae. LC-MS revealed the presence of 14 compounds in V. brasiliana crude extract, including flavonoids, flavones, sesquiterpene lactones, and quinic acids. Eriodictol (ΔGbind = −9.0), luteolin (ΔGbind = −8.7), and apigenin (ΔGbind = −8.6) obtained greater strength of molecular interaction with lanosterol demethylase in the molecular docking study. The hexane fraction of V. brasiliana showed the best leishmanicidal activity against L. amazonensis in vitro (IC50 12.44 ± 0.875 µg·mL−1) and low cytotoxicity in RAW 264.7 cells (CC50 314.89 µg·mL−1, SI = 25.30) and T. molitor larvae. However, the hexane fraction and Amphotericin-B had antagonistic interaction (FICI index ≥ 4.0). This study revealed that V. brasiliana and its metabolites are potential sources of lead compounds for drugs for leishmaniasis treatment.

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“…In vivo, a LET dose of 10 µL/kg could aid the survival of Tenebrio molitor larvae previously infected with a lethal dose of S. aureus. Hence, LET could set a propitious example of a successful antimicrobial against S. aureus [35]. It also has various medicinal uses such as in cases of cough, asthma, warts and rheumatism.…”

Section: Euphorbia Tirucalli Latexmentioning

confidence: 99%

Staphyloxanthin as a Potential Novel Target for Deciphering Promising Anti-Staphylococcus aureus Agents

et al. 2022

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Staphylococcus aureus is a fatal Gram-positive pathogen threatening numerous cases of hospital-admitted patients worldwide. The emerging resistance of the pathogen to several antimicrobial agents has pressurized research to propose new strategies for combating antimicrobial resistance. Novel strategies include targeting the virulence factors of S. aureus. One of the most prominent virulence factors of S. aureus is its eponymous antioxidant pigment staphyloxanthin (STX), which is an auspicious target for anti-virulence therapy. This review provides an updated outline on STX and multiple strategies to attenuate this virulence factor. The approaches discussed in this article focus on bioprospective and chemically synthesized inhibitors of STX, inter-species communication and genetic manipulation. Various inhibitor molecules were found to exhibit appreciable inhibitory effect against STX and hence would be able to serve as potential anti-virulence agents for clinical use.

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The latex of Euphorbia tirucalli inhibits staphyloxanthin production and protects Tenebrio molitor larvae against Staphylococcus aureus infection

Cited by 8 publications

References 12 publications

Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

Antimicrobial and Antivirulence Action of Eugenia brejoensis Essential Oil in vitro and in vivo Invertebrate Models

Chemical Characterization and Leishmanicidal Activity In Vitro and In Silico of Natural Products Obtained from Leaves of Vernonanthura brasiliana (L.) H. Rob (Asteraceae)

Staphyloxanthin as a Potential Novel Target for Deciphering Promising Anti-Staphylococcus aureus Agents

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