The Latex-Fixation Reaction in Nonrheumatic Diseases

Dresner, Ellis; Trombly, Patricia

doi:10.1056/nejm195911122612001

Cited by 105 publications

(30 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, as discussed below, much higher frequency of positivity was encountered in this category by Dresner and his co-workers. 21 Unlike their positive data in acute viral diseases, no positive reactions were found here in patients with influenza and mumps. It was disconcerting from a diagnostic standpoint to find a 5fold greater frequency of positive reactions to the macroscopic slide test (tabIe 7) than to the euglobulin latex technique in a variety of nonrheumatic diseases.…”

contrasting

confidence: 79%

Serologic methods employing F–II reactant as an aid to diagnosis of rheumatoid arthritis

Howell

Vaughn

Broome³

1961

Arthritis & Rheumatism

View full text Add to dashboard Cite

Sera of 189 patients with rheumatoid arthritis and 512 patients with variants of rheumatoid arthritis, osteoarthritis, gout, and other diseases were subjected to a battery of serologic procedures. With no apparent loss of specificity, the test employing a modified hydrochloric acid method of euglobulin fractionation produced a significantly greater frequency of positive reactions in patients with rheumatoid artbritis.Seros ab 129 patientes con arthritis rheumatoidee e ab 512 patientes con variantes de arthritis rheumatoidee, osteoarthritis, gutta, e altere morbos esseva subjicite a un batteria de manowas serologic. Sin ulle apparente perdita de specificitate, le test empleante un modification del methodo a acido hydrochloric in le fractionation de euglobulina produceva un significativemente plus alte frequentia de reactiones positive in patientes con arthritis rheumatoidee.GGLUTINATING activity of sera from patients with rheumatoid ar-A thritis studied by Cecil et al.' (1931), Waaler2 (1940), and Rose et ( 1948) initiated the discovery and characterization of the rheumatoid factor. During the last decade, studies on rheumatoid factor have probed not only its fundamental nature and significance but also its application as a diagnostic aid. Modification of rheumatoid tests have simplified and shortened the procedure. Among the host of developments in technique, some of diagnostic benefit include use of human gamma-globulin as the r e a~t a n t ,~ polystyrene latex5 or Bentonite as carrier particles," and employment of euglobulin' or gamma-globulin fractions of tested sera.* The inhibition method introduced by Ziff et al. elevates sensitivity of the sheep hemagglutination procedure,i but it is too laborious for prevalent clinical usage. Adopting the latex fixation system,5 Hall et a1.9 devised a modified inhibition test for detection of F-I1 agglutinating factors. Although simpler to perform than the test of Ziff and his co-workers,7 Hall's method retains a 48-hour dialysis period in the preparation of euglobulin. By application of a hydrochloric acid euglobulin fractionation procedure,'s the authors1" shortened this step to 1% hours. The subject of this report is clinical evaluation of a modified Hall test.1° METHODSFollowing blood collection, all sera were stored 2 to 5 days at -5 C. ' Results of only the first serum samples on a given patient are recorded here. The F-Il sheep cell (F-I1 S.From the Arthritis Section of the. Vepartment

show abstract

contrasting

confidence: 79%

Serologic methods employing F–II reactant as an aid to diagnosis of rheumatoid arthritis

Howell

Vaughn

Broome³

1961

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…These anti-y-globulins, often referred to as rheumatoid factors, are not limited in occurrence to rheumatoid arthritis, but are sometimes found in a variety of other disease states (7)(8)(9)(10). Extensive evidence is now available in support of the concept that these factors are indeed antibodies to various antigenic determinants on the yG (7 S y-globulin) (2) molecule.…”

mentioning

confidence: 99%

Characterization of the gamma-globulin complexes present in certain sera having high titers of anti-gamma-globulin activity.

Schrohenloher¹

1966

J. Clin. Invest.

View full text Add to dashboard Cite

“…While severe liver disease is frequently associated with a fall in the over-all concentration of alpha-2 globulin fraction, the presence of several well characterized proteins in this fraction, as well as the exquisite sensitivity of the test system, make a definitive characterization and accurate quantitation of the responsible ones difficult. Furthermore, the demonstration in some false positive sera of high molecular weight complexes with serologic activity similar to those seen in rheumatoid arthritis would suggest at best a secondary role for these stabilizing factors .24- 28 The relationship of the alpha-2 globulin described in this report to similar substances described in the literature is difficult to determine. It seems likely that it is closely related to the stabilizing factor I1 described by Rantz et aL3 in the latex fixation test, and possibly to a serum inhibitor for the SCA reaction described by Gray.ll On the other hand, it does not appear related to a heat-labile inhibitor described by Brine et al5 and Schubart et a1.4.1° which has some of the characteristics of complement.…”

mentioning

confidence: 73%

Stabilizers and inhibitors in the sensitized sheep‐cell agglutination reaction

Franklin

1960

Arthritis & Rheumatism

View full text Add to dashboard Cite

Study of euglobulin fractions from normal sera has demonstrated two types of inhibitors for the sheep cell agglutination reaction. One of these is a gamma globulin, the other, an alpha‐2 globulin. Gamma globulin in an aggregated state neutralizes the rheumatoid factor by combining with it. The specific constitutent of the alpha‐2 globulins responsible for these properties is not yet known, nor has it been possible to define the mechanism by which these proteins stabilize the reaction. Certain properties of these two fractions are described and their possible roles in positive reactions in certain nonrheumatoid conditions are discussed.

show abstract

The Latex-Fixation Reaction in Nonrheumatic Diseases

Cited by 105 publications

References 25 publications

Serologic methods employing F–II reactant as an aid to diagnosis of rheumatoid arthritis

Serologic methods employing F–II reactant as an aid to diagnosis of rheumatoid arthritis

Characterization of the gamma-globulin complexes present in certain sera having high titers of anti-gamma-globulin activity.

Stabilizers and inhibitors in the sensitized sheep‐cell agglutination reaction

Contact Info

Product

Resources

About