The late embryogenesis abundant gene family in tea plant (Camellia sinensis): Genome-wide characterization and expression analysis in response to cold and dehydration stress

Wang, Weidong; Gao, Tong; Chen, Jiangfei; Yang, Jiankun; Huang, Hui‐Yu; Yu, Youben

doi:10.1016/j.plaphy.2018.12.009

Cited by 55 publications

(34 citation statements)

References 46 publications

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“…Cis-acting regulatory element predictive analysis was conducted in PlantCARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/) 53 . prediction of transcription factor networks. Transcription factor network prediction was performed as described by Wang et al 54 with minor modifications. The CsJAZ CDS nucleotide sequences were submitted to the Plant Transcriptional Regulatory Map (PTRM) website (http://plantregmap.cbi.pku.edu.cn/regula-tion_prediction.php) to predict the target transcription factors of the CsJAZ genes with the following parameter: p-value ≤ 1e-6 55 .…”

Section: Exon-intron Structures Conserved Motifs and Promoter Analysismentioning

confidence: 99%

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

Chen

Wang

Sun

et al. 2020

Sci Rep

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the JASMONATE-ZIM DOMAIN (JAZ) family genes are key repressors in the jasmonic acid signal transduction pathway. Recently, the JAZ gene family has been systematically characterized in many plants. However, this gene family has not been explored in the tea plant. In this study, 13 CsJAZ genes were identified in the tea plant genome. Phylogenetic analysis showed that the JAZ proteins from tea and other plants clustered into 11 sub-groups. The CsJAZ gene transcriptional regulatory network predictive and expression pattern analyses suggest that these genes play vital roles in abiotic stress responses, phytohormone crosstalk and growth and development of the tea plant. in addition, the CsJAZ gene expression profiles were associated with tea postharvest processing. Our work provides a comprehensive understanding of the CsJAZ family and will help elucidate their contributions to tea quality during tea postharvest processing.Higher plants face a large number of severe challenges during their life cycles, including insect bites, pathogen infection, heavy metal stress, and water scarcity. However, as sessile organisms, plants have evolved sophisticated mechanisms to resist these problems and clever strategies to thrive in their ever-changing natural environments 1,2 . For plants, phytohormones are the most effective and fastest weapon in response to environmental stress. For example, jasmonic acid (JA) is widely known to play an important role in various biological processes in plants, including defense against herbivorous insect attack, flower initiation and plant morphogenesis [3][4][5] .There are at least two jasmonate synthesis pathways that exist in plants, namely octadecane pathway and hexadecanoid pathway, which begins with the release of α-linolenic acid (18:3n-3) and hexadecatrienoic acid (16:3n-3), respectively 6 . The unsaturated fatty acids are catalyzed by a series of enzymes then generates 12-oxo-10,15 (Z)-phytodienoic acid (OPDA), in the chloroplast 7 . Finally, JA is formed through OPDA reductase 3 (OPR3)-mediated reduction reaction and three rounds of β-oxidation 6 . Interestingly, an alternative pathway for JA biosynthesis was discovered. OPDA could enter the β-oxidation pathway to produce a direct precursor of JA and JA-lie in the absence of OPR3 8 . Moreover, the JA signal pathway has been deciphered 9,10 . It has been reported that the JA receptor is a co-receptor complex formed by JAZ protein, COI1 (CORONATINE INSENSITIVE1) protein and inositol pentakisphosphate 11 . Previous studies have revealed that the JA content is maintained at a relatively low level in plants under normal conditions, in which the JAZ repressor interacts with MYC2 to inhibit downstream insect-resistant or disease-resistant gene expression 12 . However, large amounts of JA accumulate in plant cells in response to abiotic or biotic stresses and are perceived by COI1 13,14 . Subsequently, JAZ proteins are degraded by COI1-mediated E3 ubiquitination. Then, the transcription activator MYC is relieved, which increases the expression o...

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Section: Exon-intron Structures Conserved Motifs and Promoter Analysismentioning

confidence: 99%

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

Chen

Wang

Sun

et al. 2020

Sci Rep

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“…CBFнезависимый путь может также включать другие гены метаболизма и транскрипционные факторы, такие как HSFC1, ZAT12 и CZF1 (Vogel et al, 2005;Park et al, 2015), PLD (фосфолипаза D) (Li et al, 2004), WRKY (Wang et al, 2016b), HD-Zip (Shen et al, 2018), LOX (Zhu et al, 2018), LEA (Wang et al, 2018), NAC (Wang et al, 2016a), которые играют важную роль в регуляции ответа на холодовой стресс. Семейства CPK (calcium dependent protein kinases) и CIPK (CBLinteracting protein kinases) -гены протеинкиназ -также рассматриваются как положительные регуляторы ответа при заморозках, поскольку экспрессия этих генов повышалась в период заморозков у Camellia japonica (Li Q. et al, 2016).…”

Section: генетические механизмы устойчивости растений чая к холодовомunclassified

“…Группа белков LEA (белки позднего эмбриогенеза животных и растений) -большое и разнообразное семейство полипептидов, играющих значительную роль в регуляции роста и развития растений. При холодовом стрессе у чая экспрессия генов этой группы повышалась, всего было выявлено 33 гена CsLEA (Wang et al, 2018).…”

Section: генетические механизмы устойчивости растений чая к холодовомunclassified

Genes underlying cold acclimation in the tea plant (<i>Camellia sinensis</i> (L.) Kuntze)

et al. 2020

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The article reviews the latest studies showing the diversity of genetic mechanisms and gene families underlying the increased cold and frost tolerance of tea and other plant species. It has been shown that cell responses to chilling (0…+15°C) and freezing (< 0°C) are not the same and gene expression under cold stress is genotype-specific. In recent decades, progress has been made in understanding the genetic mechanisms underlying the cold response of plants – ICE1 (inducer of CBF expression 1), CBF (C-repeat-binding factor), COR (cold-regulated genes) pathways and signaling have been discovered. The ICE, CBF and DHN gene groups play a key role in the cold acclimation of the tea plant. The accumulation of CBF transcripts occurs after 15 min of chilling induction, and longer cold stress leads to accumulation of CBF transcripts. It is shown that the transcripts of the CsDHN1, CsDHN2 and CsDHN3 genes accumulate at a higher level in resistant genotypes of tea in comparison with susceptible cultivars during freezing. CBF-independent pathways include genes involved in metabolism and transcription factors such as HSFC1, ZAT12, CZF1, PLD (phospholipase D), WRKY, HD-Zip, CsLEA, LOX, NAC, HSP, which are widely distributed in plants and are involved in the basic mechanisms of tea resistance to cold and frost. The most recent studies show an important role of miRNA in the mechanisms of response to chilling and freezing in tea. The data obtained on different plant species may correlate with the mechanisms of frost tolerance of tea and are the basis for future studies of the signaling pathways of response to cold in the tea plant. The results of the research emphasize the need to further explore the ways in which various genes regulate the tolerance of tea to cold stress to find the molecular markers of frost tolerance.

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“…Secondly, 'autophagy' or 'ATG' as a keyword was searched in published transcriptome data [53]. Thirdly, the nucleotide and protein sequences of ATG-related genes in Arabidopsis (44), rice (33), banana (31), and grapevine (35), tobacco (29) were retrieved from Phytozome v12.1 database (JGI, https://phytozome.jgi.doe.gov/pz/portal.html), and then they were all used as references to perform Blastn and Blastx against the TPIA database respectively. After removing redundant sequences, all of the retained protein sequences were searched in the NCBI conserved domain database (https://www.ncbi.nlm.nih.gov/Structure/bwrpsb/bwrpsb.cgi) [54] to verify the presence of ATG-related domains.…”

Section: Identi Cation Of Csargs In Tea Plantmentioning

confidence: 99%

“…For example, multiple omics techniques, including transcriptome, proteomic and metabonome have been widely used for exploring the dynamic changes of genes, proteins and the metabolites under different stress conditions [36][37][38][39][40]. In addition, many genes, which in responding to various stimulus, have been identi ed and analyzed by feat of the daft genome sequences [41][42][43][44][45][46], meanwhile, the functional studies of some difference expressed genes (DEGs), like Basic leucine zipper (bZIP) gene (CsbZIP6) [47], SWEET transporters gene (CsSWEET16) [48], vacuolar invertases gene (CsINV5) [49], and 12-Oxophytodienoate reductase gene (CsOPR3) [50], have been extensively studied. However, there has no one autophagy-related gene (ARG) been comprehensively analyzed in tea plant, and the roles of autophagy in coping with different environment stimuluses have also not yet been detailed in tea plant.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, characterization, and expression analysis of tea plant autophagy-related genes (CsARGs) reveals diverse roles during development and abiotic stress

Qian¹,

Wang²,

Ding³

et al. 2020

Preprint

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Background Autophagy, meaning ‘self-eating’, is required for degradation and recycling of cytoplasmic constituents under stressful or non-stressful conditions, thereby contributing to maintaining cellular homeostasis, delaying aged and longevity in eukaryotes. So far, the functions of autophagy have been intensively studied in yeast, mammals and model plants, but few studies have focused on economic crops, especially for tea plants, the roles of autophagy in coping with different environment stimuluses have not yet been detailed. Therefore, exploring the functions of autophagy related genes in tea plant would contribute to further understanding the mechanism of autophagy in response to stresses in woody plants. Results Here, we totally identified 35 CsARGs in tea plant. Each CsARG is highly conserved with its homologues stemmed from other plant species, except for CsATG14. Tissue-specific expression analysis revealed that the abundances of CsARGs were varied with different tissues, but CsATG8c/i showed a certain degree of tissue specificity, respectively. Under hormones and abiotic stress conditions, most of CsARGs were up-regulated at different treatment time points. In addition, the transcriptions of 10 CsARGs were higher in cold-resistance cultivar ‘Longjing43’ than the cold-susceptible cultivar ‘Damianbai’ during CA periods, however, CsATG101 showed a contrary tendency. Conclusions We comprehensively analyzed the bioinformatics and physiological roles of CsARGs in tea plant, and these results provide the basis for deepen exploring the molecular mechanism of autophagy involved in tea plant growth and development and stress responses. Meanwhile, some CsARGs would be served as putative molecular markers for cold-resistance breeding of tea plant in future.

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The late embryogenesis abundant gene family in tea plant (Camellia sinensis): Genome-wide characterization and expression analysis in response to cold and dehydration stress

Cited by 55 publications

References 46 publications

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

Genome-wide and expression pattern analysis of JAZ family involved in stress responses and postharvest processing treatments in Camellia sinensis

Genes underlying cold acclimation in the tea plant (<i>Camellia sinensis</i> (L.) Kuntze)

Genome-wide identification, characterization, and expression analysis of tea plant autophagy-related genes (CsARGs) reveals diverse roles during development and abiotic stress

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