The last Pleistocene glaciations phylogeography episode of Phaneroptera falcata (Poda, 1761) (Orthoptera: Tettigoniidae) in the Volga River basin based on the mtDNA Cytochrome C Oxidase subunit 1 (COI) gene fragment

Abstract

This study is to research the phylogeography of Phaneroptera falcata (Poda, 1761) in the Volga river basin based on the mtDNA Cytochrome C Oxidase subunit 1 (COI) gene fragment at the last Pleistocene glaciation episode. The studied location is the Volga river basin, a territory within the central and partially southern parts of European Russia; it includes the rivers Volga, Oka, Khoper and Don basins. We used the traditional molecular phylogeography methods: mtDNA COI gene fragment from the key locations within the studied area was sequenced and then analyzed (cladogram topology, haplotype diversity, cladogram calibration etc.). The phylogenetic tree shows the dispersion of our samples over the following regions: Lower Volga, Middle Volga, Lower Oka, Middle Oka, Upper Oka, Don basin, Khoper basin. Nine haplotypes determined from our samples; they are grouped into 7 haplogroups. Six of them are in the basins of the main rivers of the Volga region: three haplogroups - on the Oka (Upper, Middle and Lower Oka respectively), haplogroups of the Khoper and Don basins, and the haplogroup of the Middle Volga combining two subgroups – Lower and Middle Volga basins. The distribution of found haplogroups correlates with big river basins in the Volga area (Volga, Oka, Khoper, Don).

Diversity and Biology of Terrestrial Orthopteroids (Insecta) in the Republic of Mordovia (Russia)

“…Other species, with their activity, ability to reproduce rapidly, tolerance to adverse factors, and dispersal ability, break records of long-distance movements. Such species disperse due to human "support" [9,10]. In addition, some species are important objects of ecological research, as they reflect changes taking place in ecosystems [11,12].…”

New insights into the genetic diversity of the Balkan bush-crickets of the Poecilimon ornatus group (Orthoptera: Tettigoniidae)

“…DNA sequence analysis has revolutionized the way of recognizing species (Hajibabaei et al 2007;Taylor and Harris 2012) and helped to reveal the existence of cryptic species in many taxa (Knowlton 1993;Bickford et al 2007;Scheffers et al 2012). The cytochrome c oxidase subunit I (COI) gene is a commonly used marker, easy to amplify due to the availability of conserved primers, with a strong phylogenetic signal, used in taxonomy (Folmer et al 1994;Simon et al 1994Simon et al , 2006Spicer 1995;Zhang and Hewitt 1997;Goto and Kimura 2001;Remigio and Hebert 2003;Kjer et al 2014;Wang et al 2017;Jafari et al 2019;Karmazina et al 2020). This marker is successfully used in Orthoptera and treated as a DNA barcode (Lehmann et al 2017;Kaya and Çıplak 2018;Kundu et al 2020;Liu and He 2021;Şirin et al 2021;Warchałowska-Śliwa et al 2021).…”