The large scale antibacterial, antifungal and anti-phage efficiency of Petamcin-A: new multicomponent preparation for skin diseases treatment

Abstract

BackgroundHuman and animal skin diseases of bacterial, fungal and viral nature and their complications are widespread and globally cause a serious trouble. Their prevalence is increasing mainly due to drug resistance. Consequently, demand has increased for new effective antimicrobial drugs, which also should be less toxic, possess a wider spectrum of action and be economically more beneficial. The goal was to investigate antibacterial, antifungal and anti-phage activity of Petamcin-A-a new multicomponent preparation. It contains acetic acid and hexamethylenetetramine as main active antimicrobial components, as well as phosphatidylcholine, tocopheryl acetate and glycerol as excipients.MethodsBacteriostatic activity and minimal inhibitory concentrations of the preparation against various test-organisms were determined by agar well diffusion assay. Antifungal activity was tested by agar dilution assay. To explore anti-phage activity double agar overlay plaque assay was used. Nystatin, chlorhexidine and acetic acid were used as control agents for comparative analysis. Statistical analysis was done with GraphPad Prism 5.03 or R 3.1.0 software.ResultsThe results showed a higher activity of Petamcin-A against all bacterial and fungal test strains compared with its components or control agents. The preparation was more effective against tested gram-positive bacteria than gram-negative ones. Petamcin-A expressed bactericidal activity against almost all test strains. In addition, the preparation demonstrated high activity against T4 phage of Escherichia coli C-T4 completely inhibiting its growth. 5-fold diluted Petamcin-A also exhibited considerable activity reducing phage concentration by 2.6 Log10.ConclusionsPetamcin-A has a high antimicrobial activity against all tested strains of bacteria, yeasts and moulds. The preparation also exhibited high anti-phage activity. Moreover, taking into account that Petamcin-A has no observable toxicity on skin and its components are not expensive, it can be advantageous for management of various skin medical conditions.

Antimicrobial activity of some plant materials used in Armenian traditional medicine

“…Within tested five plant species only Rumex obtusifolius had no bactericidal activity against B. subtilis at tested concentrations. This is particularly interesting as tested B. subtilis strain could have high resistance according to our previous work [17]. Cidal activity of almost all tested plant materials toward this endospore forming bacteria also allowed us to assume that they possessed activity against endospores as well.…”

“…The antimicrobial activity of the samples were initially evaluated by modified agar well diffusion assay [17]. Growth medium (25 ml) was poured into Petri dishes at 50–70 °C and it was left to solidify under ultraviolet (UV) light (265 nm wavelength) for 15 min.…”

Lactobacilli isolated from the Armenian fermented milk product matsoun: Growth properties, antibacterial and proteolytic activity and their dependence on pH

“…To determine the antibacterial activity, modified agar well diffusion assay was used (Ginovyan et al . ). Briefly, 100 μL of overnight bacterial suspensions of each indicator strain [adjusted to 10 6 colony‐forming unit (CFU)/mL] was poured into Petri dishes.…”

“…To study the effect of the cultivation medium composition on antibacterial activity, lactobacilli were precultivated overnight in 10% skim milk and MRS broth. To determine the antibacterial activity, modified agar well diffusion assay was used (Ginovyan et al 2015). Briefly, 100 lL of overnight bacterial suspensions of each indicator strain [adjusted to 10 6 colony-forming unit (CFU)/mL] was poured into Petri dishes.…”

A Novel Topical Spray Formulation for Ginkgo Bioloba for Antifungal Activity

“…Several different antifungal preparations were used to treat various fungal infections [1,2]. They are formulated as sprays, creams, solutions, ointment, shampoos, vaginal tablets, oral medicines, and injections [3].…”