The Lactate/Pyruvate Shuttle in Spermatozoa: Operation in Vitro

Gallina, F.G.; Deburgos, N.M.G.; Burgos, C; Coronel, Carlos E.; Blanco, Antonio

doi:10.1006/abbi.1994.1072

Cited by 43 publications

(31 citation statements)

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“…The main catabolic pathway in ejaculated boar spermatozoa appears to be fermentation of extracellular glucose or fructose to lactate and, if oxygen is available, the oxidation of lactate to CO 2 and H 2 O (Jones, 1997). The latter pathway is initiated by a specific isoenzyme of lactate dehydrogenase that is localized in the mitochondria (Gallina et al, 1994), thus cutting short the transport of hydrogen from the cytoplasm into the mitochondria (Milkowski and Lardy, 1977;Jones 1997).…”

Section: Discussionmentioning

confidence: 99%

Energy metabolism and intracellular pH in boar spermatozoa

Kamp¹,

Büsselmann²,

Jones³

et al. 2003

Reproduction

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The effect of energy metabolism on intracellular pH was studied in boar spermatozoa using nuclear magnetic resonance (NMR) spectroscopy and confocal microscopy with the pH-sensitive dye seminaphthorhodafluor (SNARF-1). Freshly ejaculated spermatozoa had a high adenylate energy charge (AEC = 0.8), which decreased to 0.6 under aerobic conditions and to 0.2 under anaerobic conditions. Correspondingly, no ATP resonances but high AMP resonance were visible in 31 P-NMR-spectra of the spermatozoa. When an artificial oxygen buffer (Fluosol) and a purpose-built air supply system were used during 31 P-NMR data acquisition, ATP resonances reappeared whereas the AMP resonance disappeared. Boar spermatozoa kept under aerobic conditions have intracellular compartments that differ markedly in pH, as demonstrated by both 31 P-NMR spectroscopy and confocal microscopy. Using confocal microscopy, the midpiece of the flagellum in which all mitochondria are located was identified as an acidic compartment (pH i-mp 6.7). The intracellular pH of both the head (pH i-h ) and the long principal piece of the flagellum (pH i-pp ) were 7.2 and, thus, only slightly below the extracellular pH (pH e 7.3). Storage of spermatozoa in a glucose-free medium at 15• C when they are immotile slowly shifted the pH i-mp from 6.7 to 6.9 within 20 h, whereas pH i-h and pH i-pp remained unchanged (pH 7.1-7.2). When glucose was present in the medium, all visible compartments of the spermatozoa as well as the medium were acidified to pH 6.2 within 20 h. Under these conditions a resonance at 4.8 ppm appeared representing glycerol 3-phosphate.

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Section: Discussionmentioning

confidence: 99%

Energy metabolism and intracellular pH in boar spermatozoa

Kamp¹,

Büsselmann²,

Jones³

et al. 2003

Reproduction

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“…Regardless of whether lactate (51)(52)(53) or pyruvate (18,34,54) is the primary respiratory substrate entering mouse sperm mitochondria, the composition of the medium used in our experiments made active glycolysis a prerequisite for OXPHOS. Although the physiological (in vivo) relevance of the uptake by mitochondria of glycolytic products excreted by the principal piece is uncertain, the different regions of the mouse female reproductive tract (vagina, uterus, and oviduct) present relatively high lactate/glucose ratios around the time of ovulation (2, 7) that would enable sperm to import sufficient exogenous lactate to fuel OXPHOS (52,53,55,56).…”

Section: Differences In Sperm Energetic Metabolism Translate Into Varmentioning

confidence: 94%

Differences in ATP Generation Via Glycolysis and Oxidative Phosphorylation and Relationships with Sperm Motility in Mouse Species

Tourmente

Villar-Moya

Rial

et al. 2015

Journal of Biological Chemistry

131

141

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“…Lactate is utilized by sperm of several species, including rats, as a suitable substrate for maintaining energy production and consumption as well as oxygen consumption [2][3][4]12] . However, the major biochemical reaction-glycolysis or oxidative phosphorylation-that generates the energy required for sperm mobilization in various species remains unknown because the energyproduction pathway is species specific [13] .…”

Section: Resultsmentioning

confidence: 99%

Oxidation of Exogenous Lactate by Lactate Dehydrogenase C in the Midpiece of Rat Epididymal Sperm is Essential for Motility and Oxidative Activity

Yamashiro¹,

Toyomizu²,

Kadowaki³

et al. 2009

American J. of Applied Sciences

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Problem statement:To identify the metabolic reaction-glycolysis or oxidative phosphorylation that is mainly involved in the production of energy required for rat sperm mobilization. Approach: Epididymal sperm were collected from Wistar rats and extended in lactatecontaining or lactate-free raffinose-modified Krebs-Ringer Bicarbonate solution (mKRB)-egg yolk medium supplemented with 0, 1, 2, or 3 mM 2-Deoxy-D-Glucose (2 DG) and 1, 2, or 3 mM sodium Oxamate (OX). Sperm motility, straight-line velocity (VSL) and oxygen consumption were evaluated. Further, immunofluorescent localization of Lactate Dehydrogenase C (LDH-C) in sperm was also performed. Results: Low concentrations of 2DG (1 and 2 mM) did not significantly affect motility, VSL and oxygen consumption of sperm extended in the lactate-containing medium. While sperm motility and oxygen consumption were significantly inhibited by even 1mM 2DG in sperm extended in lactate-free medium. Sperm motility significantly inhibited in the case of sperm extended in lactatecontaining and free-medium with 1 mM OX. We also found that sperm motility was not maintained in the absence of lactate throughout the 3 h incubation period. Immunofluorescence study revealed that mainly LDH-C was may be localized in the intramitochondrial region of the sperm. Conclusion: These results suggest that exogenous lactate enhances lactate oxidation by LDH-C, thereby promoting mitochondrial oxidative reactions in the midpiece and maintaining the mobilization of rat epididymal sperm.

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The Lactate/Pyruvate Shuttle in Spermatozoa: Operation in Vitro

Cited by 43 publications

References 0 publications

Energy metabolism and intracellular pH in boar spermatozoa

Energy metabolism and intracellular pH in boar spermatozoa

Differences in ATP Generation Via Glycolysis and Oxidative Phosphorylation and Relationships with Sperm Motility in Mouse Species

Oxidation of Exogenous Lactate by Lactate Dehydrogenase C in the Midpiece of Rat Epididymal Sperm is Essential for Motility and Oxidative Activity

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