The laccase gene (LAC1) is essential for Colletotrichum gloeosporioides development and virulence on mango leaves and fruits

Wei, Yunxie; Jing, Pu; Zhang, He; Liu, Yanan; Zhou, Fangxue; Zhang, Kai‐Li; Liu, Xiaomei

doi:10.1016/j.pmpp.2017.03.005

Cited by 24 publications

(22 citation statements)

References 33 publications

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“…Colletotrichum gloeosporioides is the main causal agent of anthracnose of Mangifera indica fruits [192]. Lac1 mutants with a disrupted lac gene (LAC1) are less pigmented, rarely produce conidia, and have reduced aerial mycelial mass and radial growth rates.…”

Section: Fungal Laccases-occurrence Roles Similarities and Differementioning

confidence: 99%

Laccase Properties, Physiological Functions, and Evolution

Janusz

Pawlik

Świderska-Burek

et al. 2020

IJMS

370

242

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Discovered in 1883, laccase is one of the first enzymes ever described. Now, after almost 140 years of research, it seems that this copper-containing protein with a number of unique catalytic properties is widely distributed across all kingdoms of life. Laccase belongs to the superfamily of multicopper oxidases (MCOs)—a group of enzymes comprising many proteins with different substrate specificities and diverse biological functions. The presence of cupredoxin-like domains allows all MCOs to reduce oxygen to water without producing harmful byproducts. This review describes structural characteristics and plausible evolution of laccase in different taxonomic groups. The remarkable catalytic abilities and broad substrate specificity of laccases are described in relation to other copper-containing MCOs. Through an exhaustive analysis of laccase roles in different taxa, we find that this enzyme evolved to serve an important, common, and protective function in living systems.

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Section: Fungal Laccases-occurrence Roles Similarities and Differementioning

confidence: 99%

Laccase Properties, Physiological Functions, and Evolution

Janusz

Pawlik

Świderska-Burek

et al. 2020

IJMS

370

242

View full text Add to dashboard Cite

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“…It is noted that this trend is consistent with the plate test results (see Figure 4). Anthracnose appressorium formation is one of the most important structures invading a host [32]. Figure 6 presents OM images showing the appressorium formation of C. gloeosporioides spores contacted with the treated and untreated samples.…”

Section: Resultsmentioning

confidence: 99%

“…Among the treated samples, the sample with a 1.2-µm particle size had a particularly low melanin precipitation. Wei [32] showed that Anthracnose produce melanin to accumulate the swell pressure inside the press. In other words, the amount of melanin is one of the key factors determining the pathogenic ability of Anthracnose.…”

Section: Resultsmentioning

confidence: 99%

“…The effect of the Ag particles on the melanin synthesis behavior of the C. gloeosporioides spores was evaluated by performing the RT-PCT analysis of five related genes, namely polyketide synthase (PKS), tetra-HN reductase scytalone (THR), scytalone dehydratase (SCD), exo-glucocanase (Ecg) and pectate lyase (PEL). Seven primers were designed for the RT-PCR analysis, and 18S rRNA was selected as the internal control [32]. The RNA of the spores contacted with the different Ag particles and glass (control) sample for 15 min were extracted using an RNA purification kit (BIO-GENESIS, Germany).…”

Section: Rt-pcr Analysis Of Gene Expressionmentioning

confidence: 99%

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Antifungal Properties of Pure Silver Films with Nanoparticles Induced by Pulsed-Laser Dewetting Process

Lin

Wang

et al. 2020

Applied Sciences

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Silver particles were prepared by dewetting Ag films coated on glass using a fiber laser. The size of the particles was controlled in the range of 92 nm–1.2 μm by adjusting the thickness of the Ag film. The structural properties and surface roughness of the particles were evaluated by means of scanning electron microscopy. In addition, the antifungal activity of the Ag particles was examined using spore suspensions of Colletotrichum gloeosporioides. It is shown that particles with a size of 1.2 μm achieved 100% inhibition of conidia growth of C. gloeosporioides after a contact time of just 5 min. Furthermore, the smaller particles also achieved good antifungal activity given a longer contact time. Similar results were observed for spore germination and pathogenicity tests performed on mango fruit and leaves. Overall, the results confirm that Ag particles have an excellent antifungal effect on C. gloeosporioides.

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“…4). Anthrax appressorium formation is one of the most important structures invading the host [29]. observed that the melanin precipitation in the treated samples is greatly reduced compared to that in the untreated (control) sample.…”

Section: Rt-pcr Analysis Of Gene Expressionmentioning

confidence: 97%

Antibacterial Properties of Pure Silver Films with Nanoparticles Induced by Pulsed-Laser Dewetting Process

Wang¹,

Wang²,

Lin³

et al. 2020

Preprint

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Silver particles are prepared by dewetting Ag ﬁlms coated on glass using a fiber laser. The size of the particles is controlled in the range of 92 nm ~ 1.2 μm by adjusting the thickness of the Ag film. The structural properties and surface roughness of the particles are evaluated by means of scanning electron microscopy. In addition, the antifungal activity of the Ag particles is examined using spore suspensions of Colletotrichum gloeosporioides. It is shown that the particles with a size of 1.2 μm achieve 100% inhibition of the conidia growth of the Colletotrichum gloeosporioides after a contact time of just 5 min. Furthermore, the smaller particles also achieve a good antibacterial activity given a longer contact time. Similar results are observed in spore germination and pathogenicity tests performed on mango fruit and leaves. Overall, the results confirm that the Ag particles have an excellent antifungal effect on Colletotrichum gloeosporioides.

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The laccase gene (LAC1) is essential for Colletotrichum gloeosporioides development and virulence on mango leaves and fruits

Cited by 24 publications

References 33 publications

Laccase Properties, Physiological Functions, and Evolution

Laccase Properties, Physiological Functions, and Evolution

Antifungal Properties of Pure Silver Films with Nanoparticles Induced by Pulsed-Laser Dewetting Process

Antibacterial Properties of Pure Silver Films with Nanoparticles Induced by Pulsed-Laser Dewetting Process

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