The Laboratory Xenopus sp.

Green, Sherril L.

doi:10.1201/b13586

Cited by 40 publications

(55 citation statements)

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“…Finally, the frog was put in the water. Xenopus husbandry and all animal procedures complied with the Xenopus guidline edited by Green (2010), and all animal procedures also accorded with Regulations for the Administration of Affairs Concerning Experimental Animals (State Science and Technology Commission of the People's Republic of China, 1988).…”

Section: Experimental Animalsmentioning

confidence: 99%

Environmental (anti-)androgenic chemicals affect germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro

Cao¹,

Xu²,

Lou³

et al. 2014

Toxicology in Vitro

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Section: Experimental Animalsmentioning

confidence: 99%

Environmental (anti-)androgenic chemicals affect germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro

Cao¹,

Xu²,

Lou³

et al. 2014

Toxicology in Vitro

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“…Clinical signs of red leg, associated with this bacterium, include reddening of legs and abdomen due to the dilatation of blood capillaries, bleeding, lethargy, weight loss, fluid accumulation, and skin ulcerations (Green 2010).…”

Section: Study Area and Populationmentioning

confidence: 99%

Kin discrimination in polyphenic salamander larvae: trade-offs between inclusive fitness and pathogen transmission

Garza

Waldman

2015

Behav Ecol Sociobiol

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Some larval amphibians can develop into two distinct morphological types: a small-headed Bnormal^morph and a rarer broad-headed morph with specialized adaptations to feed on the normal morph. Cannibalism confers nutritional benefits that accelerate development, essential for survival in transient environments, but incurs potential inclusive fitness costs. Selective cannibalism of non-kin thus should be favored. However, subjects may be more vulnerable to contracting disease from non-siblings to which they lack immunological defenses. We investigated kin discrimination and pathogen transmission among Korean salamander (Hynobius leechii) larvae. We placed broad-headed morph larvae into a circular arena together with two normal morph larvae, one their sibling and the other a non-sibling. To test for kin discrimination, we recorded all behavioral interactions among them. To study pathogen transmission, we fed broad-headed larvae either a sibling or non-sibling normal larva that we previously had infected with the bacterium Aeromonas hydrophila. Two days after ingestion, we determined whether the bacterium had systemically infected cannibals by quantitative PCR (qPCR). Broad-headed larvae discriminated between sibling and non-sibling normal larvae, directing aggressive behaviors mostly toward siblings. Infection loads varied more widely among broad-headed larvae that cannibalized non-siblings than those that cannibalized siblings, but the highest infection loads were recorded after ingestion of nonsiblings. Cannibalizing non-siblings thus may increase the risk of contracting disease. Broad-headed larvae discriminate most strongly between siblings and non-siblings late in development, when inclusive fitness costs of cannibalizing relatives diminish and vulnerability to novel pathogens transmitted by non-relatives rises.

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“…Wright (1996) found that tropical species can be maintained at 21-29°C (70-85°F), while amphibians from temperate regions do well at 18-22°C (65-72°F). Tinsley (2010) recommends housing X. laevis at 18-22°C (64-72°F), while Green (2010) observes that most laboratories keep X. laevis at 21-22°C (70-72°F). Animals from temperate zones may require seasonal decreases of 5-8°C (10-15°F) (Wright, 1996).…”

Section: Temperaturementioning

confidence: 99%

“…Animals from temperate zones may require seasonal decreases of 5-8°C (10-15°F) (Wright, 1996). Silurana tropicalis is typically housed at 24-25°C (75-77°F) (Green, 2010). Mattison (1998) provides recommended temperatures for several species.…”

Section: Temperaturementioning

confidence: 99%