The Laboratory Diagnosis of Lumpy Skin Disease

Davies, F. G.; Krauss, H; Lund, Jennifer M.; Taylor, M.

doi:10.1016/s0034-5288(18)34204-8

Cited by 27 publications

(12 citation statements)

References 7 publications

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“…In an experimental infection performed by Tuppurainen (2004), antibody titers in inoculated bulls reached detection levels between days 13 and 19 pi. So, antibody levels are also related to sampling time relative to the onset of infection (Davies et al 1971;Davies 1991) which is difficult to be determined during the diagnosis of natural infections.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of different diagnostic methods for diagnosis of Lumpy skin disease in cows

Awad

Ibrahim

Mahran

et al. 2009

Trop Anim Health Prod

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Viral isolation, polymerase chain reaction (PCR), dot blot hybridization (DBH), and indirect enzyme-linked immunosorbent assay (iELISA) were used for the diagnosis of lumpy skin disease in clinically infected, fevered, and apparently normal dairy cows. Lumpy skin disease virus (LSDV) was isolated from skin biopsies and blood samples collected from clinically infected cows in percentages of 72% and 20%, respectively. The virus recovered from blood samples collected from fevered cows in percentage of 33.3%. Both PCR and DBH detected viral DNA in 100% of skin biopsies collected from clinically infected cows whereas the detection rates in blood samples collected from clinically infected animals were 100% and 84% using PCR and DBH, respectively. Viral DNA was detected in blood samples collected from fevered cows using PCR and DBH in percentages of 77.8% and 66.6%, respectively. Only 19.1% of blood samples collected from in-contact cows was positive for both of PCR and DBH. Detection rates of antibodies against LSDV using iELISA in serum samples collected from clinically infected and fevered cows were 56% and 11.1%, respectively, whereas all in-contact cows had no antibodies against the virus.

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Section: Discussionmentioning

confidence: 99%

Evaluation of different diagnostic methods for diagnosis of Lumpy skin disease in cows

Awad

Ibrahim

Mahran

et al. 2009

Trop Anim Health Prod

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“…Indirect immunofluorescent antibody (IF) technique [13] The collected tissues were taken rapidly to the freezing chamber of a cryostat and left for 15 min at -30℃. Tissues were sectioned at 3 microns thickness using a cryostat knife and then transferred to slides.…”

Section: Identification Of Causative Agentmentioning

confidence: 99%

Sequence analysis of attachment gene of lumpy skin disease and sheep poxviruses

El-Kenawy

El‐Tholoth

2010

Virol. Sin.

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In Egypt, protection of cattle against lumpy skin disease (LSD) was carried out using a sheep poxvirus (Kenyan strain) vaccination strategy. In the present study 15 skin nodules from LSD suspected cows and 5 scab samples from sheep pox (SP) suspected sheep were collected. Hyperimmune rabbit sera to Lumpy skin disease virus (LSDV)/Ismailyia88 strain and sheep pox virus (SPV)/ Kenyan vaccinal strain were prepared. The causative agent in the collected samples was identified using immunoflourescence (IF) and immunoperoxidase techniques. Of the 15 skin nodules suspected of LSD, 10 showed a positive reaction and 3 out of 5 skin scabs suspected of sheeppox were found to be positive. An antigenic correlation between field skin isolate of LSDV, tissue culture adapted LSDV/Ismailyia88 strain, field skin isolate of SPV and SPV/Kenyan vaccinal strain was studied using prepared hyperimmune sera. Also, nucleotide sequence of the PCR amplified attachment gene fragments of field skin isolate of LSDV, tissue culture adapted LSDV/Ismailyia88 strain, field skin isolate of SPV and SPV /Kenyan vaccinal strain were compared. The results revealed that the four used viruses were antigenically identical. Sequence analysis indicated that field skin LSDV isolate is more related to tissue culture adapted LSDV/Ismailyia88 strain than to vaccinal SPV/ Kenyan strain and the skin isolate of SPV is more closely related to field skin isolate of LSDV than to SPV/Kenyan vaccinal strain. Thus, further study should be applied on the advantage of a LSD vaccine prepared from LSDV in protection of cattle against LSD compared to the commonly used sheep pox vaccine.

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“…Lumpy skin disease (LSD) is a pox disease of cattle and is characterised by fever, nodules on the skin, lesions in the mouth, pharynx and respiratory tract, emaciation, enlarged lymph nodes, oedema of the skin, and sometimes death (1,2,3). The disease is one of the most important viral diseases of cattle, causing loss of condition in infected animals and permanent damage to hides.…”

Section: Introductionmentioning

confidence: 99%

Lumpy skin disease in cattle in central Ethiopia: outbreak investigation and isolation and molecular detection of the virus

Ayelet¹,

Haftu²,

Jemberie³

et al. 2014

Rev. Sci. Tech. OIE

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The study was a combination of two investigations into active outbreaks of lumpy skin disease (LSD) in cattle in central Ethiopia and a retrospective analysis of outbreak reports between January 2007 and December 2011 covering the entire country. Active outbreaks were investigated in four districts of central Ethiopia: Adama, Wenji, Mojo and Welenchiti. A semi-structured questionnaire was used to acquire data at individual and herd levels, and tissue samples were collected for viral isolation and characterisation. The retrospective analyses showed that, during the five-year period, a total of 1,675 outbreaks were reported, with 62,176 cases and 4,372 deaths. The highest number of outbreaks was reported in Oromia (1,066), followed by Amhara (365) and the Southern Nations, Nationalities and People's Region (123). Outbreaks were more frequently observed between September and December and the highest number of outbreaks was reported in 2010. During the period studied, a total of 2,174 local zebu cattle were clinically examined and morbidity and mortality rates of 13.61% (296) and 4.97% (108) were recorded, respectively. Analysis of the active outbreaks revealed a relatively consistent morbidity rate, with the highest observed in Adama (15.38%), followed by Wenji (10.26%). The highest mortality rates were also observed in Adama (5.89%) and Wenji (3.42%). The LSD virus was isolated from 22 samples and all tested positive in polymerase chain reaction analysis. The disease was observed in the cattle regardless of previous vaccination with Kenyan sheep-and goatpox vaccine; thus, vaccine efficacy was assessed under field conditions and the authors' findings, together with a possible remedy, are presented in this paper.

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The Laboratory Diagnosis of Lumpy Skin Disease

Cited by 27 publications

References 7 publications

Evaluation of different diagnostic methods for diagnosis of Lumpy skin disease in cows

Evaluation of different diagnostic methods for diagnosis of Lumpy skin disease in cows

Sequence analysis of attachment gene of lumpy skin disease and sheep poxviruses

Lumpy skin disease in cattle in central Ethiopia: outbreak investigation and isolation and molecular detection of the virus

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