The Laboratory Canine

Field, Garrett; Jackson, Todd A.

doi:10.1201/b13600

Cited by 2 publications

(2 citation statements)

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“…Dogs have well developed olfactory glands, vision, and auditory and tactile senses that allow them to gain environmental cues and information from other dogs and humans (Field and Jackson, 2006;Joint Working Group on Refinement, 2004). Dogs have well developed olfactory glands, vision, and auditory and tactile senses that allow them to gain environmental cues and information from other dogs and humans (Field and Jackson, 2006;Joint Working Group on Refinement, 2004).…”

Section: Behaviormentioning

confidence: 99%

Biology and Diseases of Dogs

Nemzek

Lester

Wolfe

et al. 2015

Laboratory Animal Medicine

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Section: Behaviormentioning

confidence: 99%

Biology and Diseases of Dogs

Nemzek

Lester

Wolfe

et al. 2015

Laboratory Animal Medicine

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“…It is difficult to determine the clinical significance of this since a standard minimum number of DCs has not been defined for effective vaccination protocols. An increased volume of blood could potentially be collected from each patient to reduce these variations, as 7.5% of circulating blood volume may be safely collected once a week, or 15% through multiple samplings over a 2-week period [11]. However, estimated blood volume may be lower in sick or older patients, and obtaining greater volumes of whole blood may not be feasible, based on the patient's weight [11].…”

Section: Discussionmentioning

confidence: 99%

Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma

Eren¹,

Hwang²,

Fidel³

et al. 2021

AJBLS

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Canine malignant melanoma is an aggressive neoplasm that carries a poor prognosis due to its minimal responsiveness to traditional therapy protocols, particularly if the oral cavity, mucocutaneous junctions, or subungual sites are involved. This proof-of-concept study evaluated a prototype autologous dendritic cell vaccine using poly-lactic-co-glycolic (PLGA) nanoparticles containing antigens from patient-derived whole tumor lysate and the adjuvant monophosphoryl lipid A in five canines with stages III-IV malignant melanoma. Nanoparticle constructs biochemical characterization; encapsulation efficiency and kinetic release studies were determined. Our results showed that tumor antigens were successfully incorporated in the PLGA/monophosphoryl lipid A nanoparticle constructs. Additional in vitro experiments showed that the PLGA/monophosphoryl lipid A nanoparticle constructs effectively activated autologous dendritic cells, and generated a greater than twofold increase in the release of the pro-immune cytokine IFN-γ. No significant adverse effects were observed in any of the patients following intradermal vaccination, and flow cytometry of whole blood revealed increased CD4:CD8 T lymphocyte ratios by the completion of the study. These results suggest that a dendritic cell vaccine utilizing PLGA/monophosphoryl lipid A nanoparticle technology could potentially initiate an adaptive immune response and is safe to administer to canine patients. Further in vivo studies with a larger cohort of patients are warranted.

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The Laboratory Canine

Cited by 2 publications

References 0 publications

Biology and Diseases of Dogs

Biology and Diseases of Dogs

Preliminary Evaluation of an Autologous Dendritic Cell Vaccine Using Nanoparticle Technology for the Treatment of Canine Malignant Melanoma

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