The labeling of cationic iron oxide nanoparticle-resistant hepatocellular carcinoma cells using targeted magnetoliposomes

Soenen, Stefaan J.; Brisson, Alain; Jonckheere, Eveline; Nuytten, Nele; Tan, Sisareuth; Himmelreich, Uwe; Cuyper, Marcel De

doi:10.1016/j.biomaterials.2010.11.005

Cited by 37 publications

(26 citation statements)

References 45 publications

(56 reference statements)

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“…Upon incubating the cells with 15 nM QDots, which is a typical QDot concentration used for cell labeling [18], confocal microscopy revealed endolysosomal localization of the QDots in all three cell types ( Figure 1A) as is commonly observed for NPs [32][33][34]. For endosomal visualization, the lipophilic dye 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO) was used.…”

Section: Resultsmentioning

confidence: 82%

The effect of nanoparticle degradation on poly(methacrylic acid)-coated quantum dot toxicity: The importance of particle functionality assessment in toxicology

et al. 2014

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Colloidal semiconductor nanoparticles (quantum dots) have attracted a lot of interest in technological and biomedical research, given their potent fluorescent properties. However, the use of heavy metal-containing nanoparticles remains an issue of debate. The possible toxic effects of quantum dots remain a hot research topic and several questions such as possible intracellular degradation of quantum dots and the effect thereof on both cell viability and particle functionality remain unresolved. In the present work, poly(methacrylic acid)-coated CdSe/ZnS quantum dots were synthesized and characterized, after which their effects on cultured cells were evaluated using a multiparametric setup. The data reveal that the quantum dots are taken up through endocytosis and when exposed to the low pH of the endosomal structures, they partially degrade and release cadmium ions, which lowers their fluorescence intensity and augments particle toxicity. Using the multiparametric method, the quantum dots were evaluated at nontoxic doses in terms of their ability to visualize labeled cells for longer time periods. The data revealed that comparing different particles in terms of their applied dose is challenging, likely due to difficulties in obtaining accurate nanoparticles concentrations, but evaluating particle toxicity in terms of their biological functionality enables an easy and straightforward comparison.

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Section: Resultsmentioning

confidence: 82%

The effect of nanoparticle degradation on poly(methacrylic acid)-coated quantum dot toxicity: The importance of particle functionality assessment in toxicology

et al. 2014

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“…The uptake of ZDS-coated NPs is lower than (but comparable to) that 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 observed for well dispersed iron oxide NPs coated with PEG 600 carboxylic acid 34 and for anionic magnetoliposomes. 30 Iron oxide NPs (11 nm) coated with dimercaptosuccinic acid showed slightly lower uptake with respect to our ZDS-coated NPs. 31 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 destiny of NPs could actually represent a great advantage in cancer therapy as lysosomaltargeted magnetic NPs were recently reported ...…”

Section: Acs Paragon Plus Environmentmentioning

confidence: 86%

“…HepG2 cells were previously used to evaluate the biocompatibility and uptake of iron oxide NPs with different coating. [30][31][32][33][34][35] To investigate the intracellular fate of NPs, we used iron oxide NPs coated with a mixture of ZDS and a fluorescein derivative (FLC) possessing a catecholic terminal for grafting to the NPs.…”

Section: Introductionmentioning

confidence: 99%

Zwitterion-Coated Iron Oxide Nanoparticles: Surface Chemistry and Intracellular Uptake by Hepatocarcinoma (HepG2) Cells

et al. 2015

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Nanoparticles (NPs) have received much attention in recent years for their diverse potential biomedical applications. However, the synthesis of NPs with desired biodistribution and pharmacokinetics is still a major challenge, with NP size and surface chemistry being the main factors determining the behavior of NPs in vivo. Here we report on the surface chemistry and in vitro cellular uptake of magnetic iron oxide NPs coated with zwitterionic dopamine sulfonate (ZDS). ZDS-coated NPs were compared to similar iron oxide NPs coated with PEG-like 2-[2-(2-methoxyethoxy)ethoxy]acetic acid (MEEA) to investigate how surface chemistry affects their in vitro behavior. ZDS-coated NPs had a very dense coating, guaranteeing high colloidal stability in several aqueous media and negligible interaction with proteins. Treatment of HepG2 cells with increasing doses (2.5-100 μg Fe/mL) of ZDS-coated iron oxide NPs had no effect on cell viability and resulted in a low, dose-dependent NP uptake, inferior than most reported data for the internalization of iron oxide NPs by HepG2 cells. MEEA-coated NPs were scarcely stable and formed micrometer-sized aggregates in aqueous media. They decreased cell viability for dose ≥50 μg Fe/mL, and were more efficiently internalized than ZDS-coated NPs. In conclusion, our data indicate that the ZDS layer prevented both aggregation and sedimentation of iron oxide NPs and formed a biocompatible coating that did not display any biocorona effect. The very low cellular uptake of ZDS-coated iron NPs can be useful to achieve highly selective targeting upon specific functionalization.

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“…Soenen et al have used magnetoliposomes that contained galactose-terminal phospholipids to promote specific uptake via asialoglycoprotein receptors. 108,109 These receptors are over-expressed on hepatocytes and hepatocyte-like cells so that systemic administration of those functionalized magnetoliposomes would allow the specific visualization of hepatocytes. 109 …”

Section: Mri Contrast Agents Targeting Specific Cell Typesmentioning

confidence: 99%

CHAPTER 11. Magnetic Resonance‐Based Cell Imaging Using Contrast Media and Reporter Genes

Velde¹,

Himmelreich²

2013

New Applications of NMR in Drug Discovery and Development

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The labeling of cationic iron oxide nanoparticle-resistant hepatocellular carcinoma cells using targeted magnetoliposomes

Cited by 37 publications

References 45 publications

The effect of nanoparticle degradation on poly(methacrylic acid)-coated quantum dot toxicity: The importance of particle functionality assessment in toxicology

The effect of nanoparticle degradation on poly(methacrylic acid)-coated quantum dot toxicity: The importance of particle functionality assessment in toxicology

Zwitterion-Coated Iron Oxide Nanoparticles: Surface Chemistry and Intracellular Uptake by Hepatocarcinoma (HepG2) Cells

CHAPTER 11. Magnetic Resonance‐Based Cell Imaging Using Contrast Media and Reporter Genes

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