The Kveim-Siltzbach test

Teirstein, Alvin S.

doi:10.1016/0738-081x(86)90046-5

Cited by 16 publications

(5 citation statements)

References 26 publications

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“…Further, there is a long history of scattered evidence in the literature of various mycobacteria being cultured from sarcoid tissue, or remnants of mycobacteria observed by EM in biopsy or autopsy specimens (53)(54)(55) . In addition, mycobacterial antigens are not easily degradable, an observation consistent with the specificity of sarcoid individuals for reacting to the Kveim-Siltzbach "antigen," a very stable crude preparation extracted from sarcoid spleen (56). Finally, for the one sarcoid individual evaluated in lung and blood, the identical Vy9 sequences were observed in both locations, consistent with the systemic nature of the disorder.…”

Section: Referencessupporting

confidence: 62%

Diversity in junctional sequences associated with the common human V gamma 9 and V delta 2 gene segments in normal blood and lung compared with the limited diversity in a granulomatous disease.

Tamura

Holroyd

Banks

et al. 1990

The Journal of Experimental Medicine

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SummaryThe T cell receptor. (TCR) junctional regions (N regions) of the common human Vy9 and V62 gene segments were sequenced from the blood and lung of normal individuals (195 transcripts) and a group of individuals with sarcoidosis (220 transcripts), a granulomatous disease in which increased numbers of Vy9+ -y/b+ T cells are often observed. In normal individuals, the vast majority (86%) ofblood Vy9 transcripts used the JyP gene segment. In contrast to this restriction ofJ region usage, there was a large diversity of the junctional region, with <20% of blood Vy9 junctional regions showing identical sequences for any one normal individual. For the blood V62 transcripts in normal individuals, there was restriction of J region usage, with 93% using J61. The junctional regions were even more diverse than for Vy9, with a unique sequence observed in each transcript examined. Compared with blood, sequences from the normal lung showed a small increase in identical junctional regions, particularly in.one individual where 46% of Vy9 transcripts examined were identical, suggesting a response of some y/6 T cells to antigens found in the lung in the normal state . In marked contrast to normals, some individuals with sarcoidosis had large numbers of Vy9 transcripts, as well as VS2 transcripts, sharing identical sequences . For Vy9 blood transcripts, two individuals showed 84 and 56% ofjunctional region sequences to be identical, respectively. Similarly, blood VS2 transcripts showed 43, 33, and 25% identical junctional region sequences in three individuals . In the sarcoid patient with the most striking over-representation of blood Vy9junctional sequences, lung Vy9 transcripts showed increased (67%) use of the same junctional region sequence as in blood. This limited diversity of TCR junctional regions among some individuals with sarcoidosis suggests a response from specific stimuli, possibly antigenic, and that y/6 T cells may play a specific role in granuloma formation in sarcoidosis, as has been suggested in other granulomatous diseases.

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Section: Referencessupporting

confidence: 62%

Diversity in junctional sequences associated with the common human V gamma 9 and V delta 2 gene segments in normal blood and lung compared with the limited diversity in a granulomatous disease.

Tamura

Holroyd

Banks

et al. 1990

The Journal of Experimental Medicine

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“…18 With correctly validated suspensions in our institution, the Kveim-Siltzbach skin test has a sensitivity Ͼ 80% and a specificity of 99.5% in the diagnosis of sarcoidosis. 19,20 We reviewed the records of all patients with proven sarcoidosis treated at the Mount Sinai Sarcoidosis Service, in New York, from January 1, 1997, to December 31, 2001. Of 354 reviewed records, two-dimensional echocardiograms were performed in 106 patients with sarcoidosis.…”

Section: Methodsmentioning

confidence: 99%

Distinctive Clinical, Radiographic, and Functional Characteristics of Patients With Sarcoidosis-Related Pulmonary Hypertension

Sulica

Teirstein

Kakarla

et al. 2005

Chest

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177

168

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“…It was performed by intradermal injection of Kveim reagent, a validated suspension of human sarcoid tissue, typically sarcoid spleen homogenised in phosphate-buffered saline (Siltzbach 1961;Teirstein 1986). The resultant papule at the injection site was biopsied 4 weeks later and presence of non-caseating granulomas indicated sarcoidosis (Kveim 1941;Chase 1961;Parrish and Turner 2009).…”

Section: The Kveim-siltzbach Testmentioning

confidence: 99%

The potential of the immunological markers of sarcoidosis in exhaled breath and peripheral blood as future diagnostic and monitoring techniques

2011

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Sarcoidosis is characterised by non-caseating granulomatous inflammation, with exaggerated immune responses at sites of disease and derangements of normal tissue architecture. The lungs are most commonly involved and progressive inflammation may result in pulmonary fibrosis. The immunopathogenesis and aetiology remain uncertain, which has made it difficult to identify a single sufficiently sensitive or specific diagnostic marker. Further investigation is needed to identify sensitive and specific markers of disease, such as in peripheral blood and exhaled breath condensate (EBC). Identification of disease markers may also be useful for investigating disease activity and predicting progression to fibrosis. This review explores the literature on the cytokine profiles of blood and bronchoalveolar lavage lymphocytes following ex vivo stimulation, as well as disease markers measured using the medium of EBC.

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The Kveim-Siltzbach test

Cited by 16 publications

References 26 publications

Diversity in junctional sequences associated with the common human V gamma 9 and V delta 2 gene segments in normal blood and lung compared with the limited diversity in a granulomatous disease.

Diversity in junctional sequences associated with the common human V gamma 9 and V delta 2 gene segments in normal blood and lung compared with the limited diversity in a granulomatous disease.

Distinctive Clinical, Radiographic, and Functional Characteristics of Patients With Sarcoidosis-Related Pulmonary Hypertension

The potential of the immunological markers of sarcoidosis in exhaled breath and peripheral blood as future diagnostic and monitoring techniques

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