The Krüppel-Like Core Promoter Binding Protein Gene Is Primarily Expressed in Placenta During Mouse Development1

Abstract

The human core promoter binding protein (hCPBP) has been identified as a DNA-binding protein involved in the regulation of TATA box-less genes like those encoding the pregnancy-specific glycoproteins. Structurally, hCPBP contains three zinc fingers in the C-terminal domain, which is highly conserved in a number of proteins that constitute the Krüppel-like family of transcription factors. In the present work, we report the molecular cloning of the mouse CPBP (mCPBP) and its expression pattern during development as well as in adult tissues. The mouse cDNA encodes a protein of 283 amino acids that share 94.4% of identity with the hCPBP. The highest level of mCPBP transcript was detected in placenta, and its expression was lower in total embryos and in adult tissues. We also show by in situ hybridization that during embryonic development the mCPBP gene is mainly expressed in extra-embryonic structures throughout gestation; essentially no specific expression was detected in embryonic tissues. Our data demonstrate that CPBP transcript is enriched in the trophoblastic tissue and strongly suggest that its encoded polypeptide regulates target genes involved in placental development and pregnancy maintenance.

A new role for the Krüppel-like transcription factor KLF6 as an inhibitor of c-Jun proto-oncoprotein function

“…KLF6 mRNA is expressed at basal levels in most tissues and at relatively high levels in human and mice placenta (Koritschoner et al, 1997;Slavin et al, 1999). KLF6 protein expression is detected as two bands of 42 and 46 kDa in several tissues and cells lines (Kojima et al, 2000;Narla et al, 2001, this work) showing different intensity in Western blot assays probably reflecting differences in post-translational modifications.…”

Biology of Krüppel‐like factor 6 transcriptional regulator in cell life and death

“…7. ) placenta, mainly at term, which constitutes the specific site for PSG expression (10)(11)(12). These proteins, structurally related to the carcinoembryonic antigen, are highly expressed during normal trophoblast development and functionally contribute to the immune privilege of placental tissue during pregnancy (13).…”

“…Additionally, the klf62/2 mice phenotype is characterized by developmental defects including impaired placental development and poorly defined liver contributing to early lethality along with failures in the yolk sac vascularization (15). Besides the enriched KLF6 expression in placenta, its transcript is also detected in all mammalian tissues, although at lower levels; whereas, marginal expression was detected at the embryo proper during mice embryogenesis (12).…”

Activating transcription factor 3 (ATF3) promotes sublytic C5b-9-induced glomerular mesangial cells apoptosis through up-regulation of Gadd45α and KLF6 gene expression

“…KLF6 contains three C 2 H 2 zinc fingers at the C-terminal domain, recognizes GC or CACCC motifs, and is broadly expressed in numerous cell types at various developmental stages (Slavin et al 1999). KLF6 activation can result in cell cycle arrest, Effects of Gadd45␣ or KLF6 on sublytic C5b-9-induced GMCs apoptosis.…”