The Kinetics of Synthesis of Infectious Unintegrated Virus DNA in Rous Sarcoma Virus Infected Chicken Cells

N, Stedman; Mariage, Regine; Goubin, Gérard; Hillova, Jana; Hill, M.

doi:10.1099/0022-1317-42-1-207

Cited by 5 publications

(3 citation statements)

References 2 publications

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“…Further DNA preparations were purified from mixedly double-infected chicken cells. In these cells, in contrast, both RAV-1 and SR-E repli cated simultaneously and, in all probability [22,30], proviruses of these viruses were both synthesized, processed and integrated into the cellular chromosome at the same time.…”

Section: Infect Ivity Of Dna Purified From Superinfected and Double-imentioning

confidence: 99%

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Tandem Integration of Avian Retroviruses in Double-Infected Cells

Carloni¹,

N²,

Hillova³

et al. 1980

Intervirology

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Chicken embryo fibroblasts were chronically infected by both RAV-1 and SR-E, and established SR-E-transformed quail cells were superinfected with RAV-1. About 75 and 50% of double-infected and superinfected cells, respectively, produced both parental viruses. The DNA purified from these cells contained infectious provirus of both parents and no detectable recombinant provirus. In assays using agar overlay, about 5% of the transformed foci that were induced by the DNA of double-infected, but not superinfected, cells were found to contain the progeny of both parents. This observation suggests that in double infections with avian retroviruses, DNA of both parent viruses may integrate into the host genome either in tandem or in close proximity.

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Section: Infect Ivity Of Dna Purified From Superinfected and Double-imentioning

confidence: 99%

“…The infectious cen ter technique of Tentin and Rubin [20] . DNA was extracted from the Hirt supernatant of !08 cells as described [30] and, in lane A. 20 |jtg were subjected to electrophoresis.…”

Section: Fraction O F Double-infected Cellsmentioning

confidence: 99%

Tandem Integration of Avian Retroviruses in Double-Infected Cells

Carloni¹,

N²,

Hillova³

et al. 1980

Intervirology

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“…The pellet was dissolved and incubated overnight in 0.1 M NaCI, 0.05 A/Tris-HCl, 0.01 M EDTA solution containing 100 pg proteinase K per ml and 1% SDS at 37°. DNA was purified using the conventional phenol-chloroform deproteinization and RNase digestion steps as de scribed elsewhere [11].…”

Section: Metaphase Arrest and Chromosome Fractionationmentioning

confidence: 99%

RSV Provirus with Same Flanking Sequences Is Found on Different Size Classes of Chinese Hamster Chromosomes

Hillova¹,

Hill²,

Mariage‐Samson³

et al. 1985

Intervirology

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Rous sarcoma virus (RSV)-transformed Chinese hamster fibroblasts, containing approximately ten copies of the DNA domain comprising a single provirus and its flanking cellular sequences, were arrested in metaphase, and the chromosomes were fractionated by size in a sucrose gradient. The resolution of polymorphic ribosomal genes, the dihydrofolate reductase gene, and the c-src gene demonstrated that the gradient can distinguish between small, medium, and large chromosomes. The same DNA domain carrying the RSV provirus was found to be associated with chromosomes of all three size classes. Polymorphic copies of the domain in small and large chromosomes could be distinguished from those in medium-sized chromosomes because of the polymorphism in the Xhol and EcoRl sites on 5’ and 3’ adjacent cellular sequences, respectively. The presence of the same provirus domain on different chromosomes, together with the karyological data showing trisomies in the same chromosome size classes, suggest that the provirus domain, possibly the entire replicon, was duplicated and transferred to different nonhomologous chromosomes, and the transfer was followed by duplication of the target chromosomes. The possibility that proviral long terminal repeats might be involved in replicon transfer is discussed.

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