The kinetics of DNA damage by bleomycin in mammalian cells

López-Larraza, Daniel M.; Luca, J.C. De; Bianchi, Néstor O.

doi:10.1016/0027-5107(90)90110-p

Cited by 58 publications

(21 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It could be said that this concentration of zeocin marked the beginning of a plateau because there was no statistically significant difference between further concentrations (P > 0.05). These findings are in accordance with other results [29] that low doses of bleomycin (1-5 µg/ml) and short treatments (5-15 min) produced marked DNA cleavage in mammalian cells, whereas high doses and longer treatment times lead to a relatively small increases in DNA damage above these levels. The comet assay can detect very low level of damage from about 100 up to 3000 breaks per cell and thus these results reflect to the assay limitation where with increased dose the plateu is reached.…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes

Hudecová¹,

Hašplová²,

Kellovska³

et al. 2012

neo

View full text Add to dashboard Cite

Zeocin is a member of bleomycin/phleomycin family of antibiotics isolated from Streptomyces verticullus. This unique radiomimetic antibiotic is known to bind to DNA and induce oxidative stress in different organisms producing predominantly single-and double-strand breaks, as well as a DNA base loss resulting in apurinic/apyrimidinic (AP) sites. The aim of this study was to induce an adaptive response (AR) by zeocin in freshly isolated human lymphocytes from blood and to observe whether plant extracts could modulate this response. The AR was evaluated by the comet assay. The optimal conditions for the AR induction and modulation were determined as: 2 h-intertreatment time (in PBS, at 4°C) given after a priming dose (50 µg/ml) of zeocin treatment. Genotoxic impact of zeocin to lymphocytes was modulated by plant extracts isolated from Gentiana asclepiadea (methanolic and aqueous haulm extracts, 0.25 mg/ml) and Armoracia rusticana (methanolic root extract, 0.025 mg/ml). These extracts enhanced the AR and also decreased DNA damage caused by zeocin (after 0, 1 and 4 h-recovery time after the test dose of zeocin application) to more than 50%. These results support important position of plants containing many biologically active compounds in the field of pharmacology and medicine.

show abstract

Section: Discussionsupporting

confidence: 93%

“…invivogen.com). It is known that bleomycin induces oxidative DNA damage and DNA cleavage in mammalian cells [29]. Our results, measured by the comet assay, showed that zeocin similarly to bleomycin causes DNA damage in freshly isolated human lymphocytes (Fig.…”

Section: Discussionsupporting

confidence: 57%

Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes

Hudecová¹,

Hašplová²,

Kellovska³

et al. 2012

neo

View full text Add to dashboard Cite

show abstract

“…BLM acts by generating oxygen radical species and it can be destroyed by the reactive oxygen species produced by itself (Scheulen et al, 1981;Lopez-Larraza et al, 1990). Salvadori et al (1994) suggested that the presence of a scavenger, such as ascorbic acid and β-carotene, could cancel or reduce this detoxification.…”

Section: Discussionmentioning

confidence: 99%

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Araújo

Dias²,

Kronka

et al. 1999

Genet. Mol. Biol.

View full text Add to dashboard Cite

Cell culture and treatmentsChinese hamster ovary cells (CHO-9 cell line) were kindly supplied by Prof. A.T. Natarajan (University of Leiden, The Netherlands). Cells were maintained as monolayers growing at 37°C in 25-cm 2 flasks (Corning) containing HAM-F10 (Sigma) plus DMEM (Dulbecco's modi- ABSTRACT Naturally occurring antioxidants have been extensively studied for their capacity to protect organisms and cells from oxidative damage. Many plant constituents including turmeric and curcumin appear to be potent antimutagens and antioxidants. The effects of turmeric and curcumin on chromosomal aberration frequencies induced by the radiomimetic agent bleomycin (BLM) were investigated in Chinese hamster ovary (CHO) cells. Three concentrations of each drug, turmeric (100, 250 and 500 µg/ ml) and curcumin (2.5, 5 and 10 µg/ml), were combined with BLM (10 µg/ml) in CHO cells treated during the G 1 /S, S or G 2 /S phases of the cell cycle. Neither turmeric nor curcumin prevented BLM-induced chromosomal damage in any phases of the cell cycle. Conversely, a potentiation of the clastogenicity of BLM by curcumin was clearly observed in cells treated during the S and G 2 /S phases. Curcumin was also clastogenic by itself at 10 µg/ml in two protocols used. However, the exact mechanism by which curcumin produced clastogenic and potentiating effects remains unknown.

show abstract

“…La bleomicina es un antibiótico citotóxico antitumoral que exhibe un doble efecto: por un lado induce degradación del ADN, 15 y por otro, presenta efecto esclerosante sobre el endotelio vascular, cualidad que le valió su uso intralesional en las ML 13 y otras malformaciones vasculares, hemangiomas y derrame pleural neoplásico. 16 Nosotros optamos por bleomicina por su amplia disponibilidad, bajo costo y mínimos efectos adversos.…”

Section: Discussionunclassified

Malformaciones linfáticas: tratamiento percutáneo con bleomicina

Cuervo

Galli²,

Eisele³

et al. 2011

Arch Argent Pediat

View full text Add to dashboard Cite

Arch Argent Pediatr 2011;109(5):417-422 / 417 RESUMEN Las malformaciones linfáticas son anomalías del desarrollo del sistema linfático que tienden a sufrir complicaciones en su evolución. En la última década, la terapia con agentes esclerosantes ha ido ganando popularidad sobre la cirugía, por su eficacia, sus menores complicaciones y sus excelentes resultados estéticos. Presentamos una serie de 24 pacientes tratados mediante esclerosis percutánea con bleomicina. Los resultados fueron excelentes (reducción de volumen ≥ 95% y asintomáticos) en 12 pacientes, buenos (reducción de volumen entre 50% y 95% y asintomáticos) en 5 pacientes, regulares (reducción de volumen < 50% y asintomáticos) en 4 pacientes, y malos (sin cambios de volumen respecto al inicio o sin desaparición de síntomas iniciales) en 3 pacientes. Estos resultados estuvieron directamente relacionados con el tipo de malformación. Las complicaciones observadas fueron de escasa relevancia clínica. La escleroterapia con bleomicina es un método eficaz y seguro en el tratamiento de pacientes con malformación linfática uniquística o macroquís-tica. Su indicación también podría extenderse a pacientes con malformación linfática microquís-tica o cavernosa que serán sometidos a cirugía, para obtener una reducción de su tamaño y menor linforragia postoperatoria, y a pacientes postquirúrgicos sintomáticos, en quienes no fue posible la resección total de la malformación. Palabras clave: malformación linfática, escleroterapia percutánea, linfangioma, malformación vascular. SUMMARYLymphatic malformations are developmental abnormalities of the lymphatic system, which tend to complicate during their evolution. In the last decade, therapy with sclerosing agents has gained popularity over surgery due to its effectiveness, fewer complications, and excellent cosmetic results. We present a series of 24 patients treated with percutaneous bleomycin injection. Results were excellent (volume reduction ≥ 95%, without symptoms) in 12 patients, good (volume reduction between 50% and 95%, without symptoms) in 5 patients, fair (volume reduction <50%, without symptoms) in 4 patients, and poor (no change in volume from baseline and persistence of symptoms) in 3 patients. These results were directly related to the type of lymphatic malformation. The complications found had little clinical relevance. Sclerotherapy with bleomycin is an effective and safe treatment for patients with unicystic or macrocystic lymphatic malformations. It could also be used in patients with microcystic or cavernous lymphatic malformation undergoing surgery to reduce both the malformation size and postoperative lymphorrhea, and in postoperative symptomatic patients whose malformation could not be totally resected. INTRODUCCIÓNLas malformaciones linfáticas (ML) son anomalías del desarrollo normal del sistema linfático y representan una de las formas más frecuentes de malformación vascular. 1Existen publicaciones que sugieren una conducta expectante para las ML asintomáticas, 2 pero la mayoría de los autores c...

show abstract

The kinetics of DNA damage by bleomycin in mammalian cells

Cited by 58 publications

References 9 publications

Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes

Gentiana asclepiadea and Armoracia rusticana can modulate the adaptive response induced by zeocin in human lymphocytes

Effects of turmeric and its active principle, curcumin, on bleomycin-induced chromosome aberrations in Chinese hamster ovary cells

Malformaciones linfáticas: tratamiento percutáneo con bleomicina

Contact Info

Product

Resources

About