The kinetics of carboxymethylcellulose–ficin in packed beds

Abstract

1. The kinetics of the hydrolysis of benzoylarginine ethyl ester in packed columns of CM-cellulose-70-ficin and CM-cellulose-90-ficin were studied. 2. The apparent Michaelis constant, K'(m), of these preparations was calculated and shown to be dependent on the flow rate at low rates of perfusion through the columns. 3. The values for k(3) of these preparations were calculated and shown to be nearly independent of flow rate. 4. A modified form of the integrated Michaelis rate equation was used to describe the action of these materials and its limitations are discussed. 5. The hydrolysis of solutions of casein by these columns was studied.

Sucrose Hydrolysis in a Bespoke Capillary Wall-Coated Microreactor

“…Frequently, enzyme kinetics is evaluated in transient or pre-steady state conditions by stopped-flow or chemical quench-flow methods [42,45,46]. However, when the development and characterization of continuous flow reaction system is aimed, such methodologies fail to achieve adequate kinetic evaluation because flow rates affect the rate of diffusion of substrate into the surroundings of the enzyme [13,15,47,48]. In order to overcome this issue, Lilly et al introduced a model that allows evaluating the kinetic parameter of a biocatalyst in continuous mode [48].…”

“…However, when the development and characterization of continuous flow reaction system is aimed, such methodologies fail to achieve adequate kinetic evaluation because flow rates affect the rate of diffusion of substrate into the surroundings of the enzyme [13,15,47,48]. In order to overcome this issue, Lilly et al introduced a model that allows evaluating the kinetic parameter of a biocatalyst in continuous mode [48]. Additionally, this model assesses the impact of flow rates on the mass transfer effects.…”

Microfluidic Multiple Cell Chip Reactor Filled with Enzyme-Coated Magnetic Nanoparticles — An Efficient and Flexible Novel Tool for Enzyme Catalyzed Biotransformations

“…The linear fitting method proposed by Lilly, et al [14] was applied for the calculation of the kinetic constants of the biotransformation of L-1a to 2a (Figure 6, bottom). The values of the kinetic constants are summarized in Table 2.…”

“…Let P* be the fraction of substrate reacted in the reactor volume: According to Lilly et al [14], the kinetics of an insoluble enzyme constituting filled reactor is the following:…”

Biomicrofluidic chemoemitter systems: towards pheromone communication

“…Fundamental research had been conducted by Lilly-Hornby, in their work they described a method to achieve the enzyme kinetics in a flow-through microreactor system without varying the concentration of substrate (46). As one of the conclusions from that work was that the rate of reaction was affected by rate of diffusion to the active site of the enzyme and away.…”