The kinetic measurement of phagocyte function in whole blood

Boghossian, S.; Wright, G. M.; Segal, Anthony W.

doi:10.1016/0022-1759(83)90341-1

Cited by 8 publications

(2 citation statements)

References 25 publications

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“…1981: Nishiya & Horwitz, 1982Veselsky, 1981;Ashorn et al, 1986). Its role in phagocytic processes (Boghassion et al, 1983) in the male reproductive tract needs to be explored.…”

Section: Discussionmentioning

confidence: 99%

“…lactoferrin in the modulation of immunological responses has been proposed at various targets: modulation of C3-convertase of complement (Kijlstra & Jeurissen, 1982), enhancement of monocyte natural killer activity, and inhibition of antibodydependent cellular cytotoxicity (ADCC) (Nishiya & Horwitz, 1982). The latter is probably mediated by receptor-type binding of lactoferrin to the monocytes (Birgens et al, 1983), or by modulation of antibody response (Duncan & McArthur, 1981) through an effect on macrophages, such as receptor binding (van Snick et al, 1974), opsonization (Markowetz, van Snick & Masson, 1979), phagocytosis (Boghassion, Wright & Segal, 1983), or migration of mononuclear cells into acute inflammation lesions (de Sousa et al, 1978). Lactoferrin may suppress lymphoblast proliferation in vitro (Ashorn et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Localization of lactoferrin in the male reproductive tract

Wichmann

Vaalasti

et al. 1989

Int J of Andrology

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The immunohistochemical localization of lactoferrin in the normal human prostate, seminal vesicle, vas deferens, epididymis and testis was studied using the peroxidase-antiperoxidase method at the light and electron microscopical level. Lactoferrin immunoreactivity was localized in the glandular epithelial cells and granulocytes in the prostate and seminal vesicle. In the prostate, lactoferrin showed an uneven distribution; some of the glands contained exclusively positive cells and others were completely lactoferrin negative, while the rest contained scattered positive cells. The seminal vesicles were divided into three segments, and their lactoferrin content varied significantly although it was always epithelial. The ductus deferens, epididymis and testis contained no lactoferrin. In conclusion, lactoferrin was found in the prostate and seminal vesicles, but not in the testis.

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“…1981: Nishiya & Horwitz, 1982Veselsky, 1981;Ashorn et al, 1986). Its role in phagocytic processes (Boghassion et al, 1983) in the male reproductive tract needs to be explored.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Localization of lactoferrin in the male reproductive tract

Wichmann

Vaalasti

et al. 1989

Int J of Andrology

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Phagocytosis, intracellular pH, and cell volume in the multifunctional analysis of granulocytes by flow cytometry

Rothe

Valet

1988

Cytometry

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Phagocytosis of Escherichia coli KlZ strain bacteria was used to measure by flow cytometry the functional activities of human granulocytes in whole blood or huffy coat preparations. In a first measurement, the increase in electric cell volume and acridine orange (AO) green and red fluorescence were used to quantify the degree of phagocytosis. In a second measurement, the intracellular pH and esterase activity of each cell were determined with 1,4-diacetoxy-2,3-dicyanobenzene to obtain information on the metabolic activities during phagocytosis and degradation of bacteria. The DNA of dead cells was simultaneously counterstained with propidium iodide in both assays. The volume, the A 0 green and red fluorescence, the internal pH, and esterase activity were automatically averaged for all granulocytes or lymphocytes of a measurement. The calculated mean values were transferred into the self-learning database of the DIAGNOS1-program system. The functional granulocyte parameters of normal healthy individuals can be used as reference values for the automated diagnosis of abnormal granulocytes in various infectious disease states. The assays require 1 ml of heparinized whole blood and the results are available within 1 hour.Key terms: Neutrophils, phagocytosis, cytoplasmic hydrogen-ion concentration, cell volume Granulocytes play an important role in the defense of the organism through phagocytosis and degradation of bacteria, viruses, or fungi and through endocytosis of immune complexes. The number and activity of granulocyte change during severe infection (1,2,3),.and sepsis (6,32), but also after major surgery (9,401, polytrauma, and thermal injury (5,Zl).Activated granulocytes cause endothelial damage by the release of proteinases and oxygen radicals in the adult respiratory distress syndrome (41) and during hemodialysis (18).Analysis of granulocyte activation encounters difficulties in research and clinical laboratories because of the lack of fast, sensitive, and reliable in vitro methods for measurement of granulocyte functions.Microbiological methods require plating and culture of bacteria surviving after phagocytosis on agar plates (15). Microscopic methods for the quantification of phagocytosis (27) are time-consuming. In cuvette assays, only the total amount of phagocytosis but not the activity of single cells or granulocyte subpopulations can be measured. Chemiluminescence (11, the clearance of [140C]-labelled bacteria from suspension (4), the incorporation of t3H]-uridine into extracellular bacteria (26), the uptake of fluorescent latex beads, heat-killed bacteria coupled with FITC (231, latexbeads coupled to fluorogenic substrates (33), or phagocytosis of oil particles containing Oil Red 0 (7) are determined in such assays with suspensions of isolated granulocytes. The isolation of granulocytes by ficoll-hypaque or dextran sedimentation has been shown to induce spontaneous depolarization of the transmembrane potential (28), increased binding of C3b-coated microspheres, and morphological alteration of the ce...

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Recent Progress in the Quantitation of Phagocytosis

Jungi¹

1988

Reviews on Immunoassay Technology

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The kinetic measurement of phagocyte function in whole blood

Cited by 8 publications

References 25 publications

Localization of lactoferrin in the male reproductive tract

Localization of lactoferrin in the male reproductive tract

Phagocytosis, intracellular pH, and cell volume in the multifunctional analysis of granulocytes by flow cytometry

Recent Progress in the Quantitation of Phagocytosis

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