The key role of rubella virus glycoproteins in the formation of immune response, and perspectives on their use in the development of new recombinant vaccines

Petrova, E.K.; Dmitrieva, Anastasia A.; Trifonova, E. A.; Nikitin, Nikolai A.; Карпова, О. В.

doi:10.1016/j.vaccine.2016.01.010

Cited by 12 publications

(11 citation statements)

References 85 publications

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“…Antibodies to the structural proteins—specifically to the surface glycoproteins E1 and E2—are expected to be associated with neutralizing Ab response (as found in our study), since E1 and E2 contain the known neutralization epitopes/specificities. [ 48 ] Our study also demonstrates a previously unknown association between antibodies directed against non-structural rubella virus proteins (i.e., P150) and neutralizing Ab response. In concert with this finding, studies of other single-stranded positive polarity RNA viruses (West Nile, yellow fever, dengue, and tick-borne encephalitis viruses) have presented evidence for the protective role of antibodies to viral non-structural proteins in animal models, hence the assessment of humoral immunity to these proteins should be considered in systems biology, seroprevalence and/or other vaccine studies.…”

Section: Resultssupporting

confidence: 63%

“…[ 7 , 8 , 9 , 10 , 11 , 12 ] While antibodies to E1 are functional in virus neutralization by hampering virus attachment/cell entry and E1 conformational changes/trimerization during cell entry, the neutralization mechanism of anti-E2 antibodies remains largely unknown. [ 48 ] Glycoprotein E2 function warrants additional studies, but this protein is likely involved in conformational changes during virus entry and maturation, E1 activation, E1 trafficking, and virus membrane budding. [ 48 ] Of interest, antibodies directed to E2 are more prevalent in individuals with CRS compared to vaccinations and non-CRS rubella infections, [ 53 , 54 ] and thus can be used, in combination with other antibodies, as a potential marker of rubella virus-specific pathology (CRS).…”

Section: Resultsmentioning

confidence: 99%

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Characterization of rubella-specific humoral immunity following two doses of MMR vaccine using proteome microarray technology

et al. 2017

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Introduction//BackgroundThe lack of standardization of the currently used commercial anti-rubella IgG antibody assays leads to frequent misinterpretation of results for samples with low/equivocal antibody concentration. The use of alternative approaches in rubella serology could add new information leading to a fuller understanding of rubella protective immunity and neutralizing antibody response after vaccination.MethodsWe applied microarray technology to measure antibodies to all rubella virus proteins in 75 high and 75 low rubella virus-specific antibody responders after two MMR vaccine doses. These data were used in multivariate penalized logistic regression modeling of rubella-specific neutralizing antibody response after vaccination.ResultsWe measured antibodies to all rubella virus structural proteins (i.e., the glycoproteins E1 and E2 and the capsid C protein) and to the non-structural protein P150. Antibody levels to each of these proteins were: correlated with the neutralizing antibody titer (p<0.006); demonstrated differences between the high and the low antibody responder groups (p<0.008); and were components of the model associated with/predictive of vaccine-induced rubella virus-specific neutralizing antibody titers (misclassification error = 0.2).ConclusionOur study supports the use of this new technology, as well as the use of antibody profiles/patterns (rather than single antibody measures) as biomarkers of neutralizing antibody response and correlates of protective immunity in rubella virus serology.

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Section: Resultssupporting

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Characterization of rubella-specific humoral immunity following two doses of MMR vaccine using proteome microarray technology

et al. 2017

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“…Endoplasmic reticulum, membranous networks of the cell, is a crucial organelle used for viral entry and viral replication. Rubella virus possesses the ability to rearrange cellular membranes to facilitate its viral replication (Lee & Bowden, 2000, Petrova et al, 2016. The endoplasmic reticulum, Golgi complex, and mitochondria are often closely arranged around the virus replication complex, in RV infected SIRC cells (Lee & Bowden, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…The vaccine is a live attenuated preparation of the virus (RA 27/3), which induces immunity by producing a modified rubella infection (Parkman, 1996). The vaccines produced by attenuated rubella virus are effective, however possess some side effects and are uneffective for pregnant women and immunodeficiency people (Petrova et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Antiviral Activity of Geopropolis Extract from Scaptotrigona Aff. Postica against Rubella Virus

Coelho¹,

Figueiredo²,

Negri³

et al. 2018

JFR

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The search for functional foods, which possess bioactive substances, is a new trend for the obtention of alternative and more effective treatments of many diseases with fewer side effects. Geopropolis, elaborated by stingless bees, is a mixture of plant resin sources, wax and soil. In the geopropolis from Scaptotrigona affinis postica (Latreille, 1807), (Hymenoptera, Apidae, Meliponini) was not observed the presence of soil. In a previous study, the extract of geopropolis provided by the beekeeper, from S. postica of Barra do Corda, Maranhão State, exhibited potent antiviral activity against herpes simplex virus. In this study, the propolis extract was prepared experimentally and characterized by RP-HPLC-DAD-ESI-MS/MS. The objective of this study was to evaluate the antiviral activity of an experimentally prepared geopropolis extract from S. postica against Rubella Virus infected Statens Serum Institute Rabbit Cornea (SIRC) cells. Rubella virus infection of susceptible women during the first trimester of pregnancy, often results in a combination of birth defects in newborns. There is not an effective treatment for rubella virus infection. Different protocols were carried out to evaluate, the antiviral effect of geopropolis extract on the viral replication of infectious RV. Cell viability and cell proliferation assays indicated that this geopropolis was not toxic to cultured SIRC cells. In the viral binding assay, antiviral assay, real-time PCR, and transmission electron microscopy, was observed that different concentrations of geopropolis (17, 34 and 68 µg/mL) was able to inhibit the binding of virions to the cell receptor and the production of infectious RV particles in post treated and pre treated infected SIRC cells. The antiviral activity could to be attributed to the high contents of the apigenin derivatives, vicenin-2 and schaftoside. As far as we know, this is the first report about the antiviral activity of geopropolis from Scaptotrigona postica against a Togaviridae virus.

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“…Rubella is highly infectious disease transmitted by respiratory droplet, usually causes mild disease in children with symptoms of low grade fever, maculopapular rash, arthralgia and myalgia [9][10][11]. However infection of women during pregnancy especially in first 12 weeks may cause death of fetus or Congenital Rubella Syndrome which has different clinical manifestations such as sensory neural deafness, congenital cataract and glaucoma, microcephaly and heart disease that occur in 80%-90% of cases [9,10].The immune response in human body developed after vaccination and infection, with formation of antibodies against structural protein [12][13][14][15], which directed mainly toward E1, E2 and capsid protein. The antibodies against E1 glycoprotein persist *Corresponding author: Shaima Nasr Eldeen Mohamed Elgenaid, Department of Health, Faculty of Medicine, University of Khartoum, Khartoum, Sudan, Tel: 00249925117020; E-mail: shema2690@gmail.com…”

Section: Introductionmentioning

confidence: 99%

Prediction of Multiple Peptide Based Vaccine from E1, E2 and Capsid Proteins of Rubella Virus: An In-Silico Approach

Elgenaid¹,

Al-Hajj²,

Ibrahim³

et al. 2018

Immunome Res

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Rubella is a single strand RNA virus in structure that belongs to Togaviridae family. It causes rubella by respiratory droplet transmission and congenital rubella syndrome if infection to the mother occurs during pregnancy. The current life attenuated vaccine is given as part of MMR vaccine. It has many side effects and contraindicated in pregnancy and immunosuppressed persons. The aim of this study is to determine antigenic peptides from E1, E2, and Capsid proteins that can be used for multiple peptide vaccine design using In-Silico study. A total of 189 sequences of three proteins were obtained from NCBI and subjected to multiple sequence alignments using CLUSTALW tool to determine conserved regions.

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The key role of rubella virus glycoproteins in the formation of immune response, and perspectives on their use in the development of new recombinant vaccines

Cited by 12 publications

References 85 publications

Characterization of rubella-specific humoral immunity following two doses of MMR vaccine using proteome microarray technology

Characterization of rubella-specific humoral immunity following two doses of MMR vaccine using proteome microarray technology

Antiviral Activity of Geopropolis Extract from Scaptotrigona Aff. Postica against Rubella Virus

Prediction of Multiple Peptide Based Vaccine from E1, E2 and Capsid Proteins of Rubella Virus: An In-Silico Approach

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