The keratin-desmosome scaffold of internal epithelia in health and disease – The plot is thickening

Toivola, Diana M.; Polari, Lauri; Schwerd, Tobias; Schlegel, Nicolas; Strnad, Pavel

doi:10.1016/j.ceb.2023.102282

Current Opinion in Cell Biology

2024

DOI: 10.1016/j.ceb.2023.102282

|View full text |Cite

The keratin-desmosome scaffold of internal epithelia in health and disease – The plot is thickening

Diana M. Toivola,

Lauri Polari,

Tobias Schwerd

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Identification and culture of functional salivary gland ductal epithelial cells

Zhang,

Zhao,

Tao

et al. 2024

Histochem Cell Biol

View full text Add to dashboard Cite

Identification and culture of functional salivary gland ductal epithelial cells

Zhang,

Zhao,

Tao

et al. 2024

Histochem Cell Biol

View full text Add to dashboard Cite

β-Cell keratin 8 maintains islet mechanical integrity, mitochondrial ultrastructure, and β-cell glucose transporter 2 plasma membrane targeting

Baghestani,

Haldin,

Kosijer

et al. 2024

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Islet β-cell dysfunction is an underlying factor for type I diabetes (T1D) development. Insulin sensing and secretion is tightly regulated in β-cells at multiple subcellular levels. The epithelial intermediate filament protein keratin (K) 8 is the main β-cell keratin, constituting the filament network with K18. To identify the cell-autonomous functions of K8 in β-cells, mice with targeted deletion of β-cell K8 (K8flox/flox; Ins-Cre) were analyzed for islet morphology, ultrastructure and integrity, as well as blood glucose regulation and streptozotocin (STZ)-induced diabetes development. Glucose transporter 2 (GLUT2) localization was studied in β-cells in vivo and in MIN6 cells with intact or disrupted K8/K18 filaments. Loss of β-cell K8 leads to a major reduction in K18. Islets without β-cell K8 are more fragile and these β-cells display disjointed plasma membrane organization with less membranous E-cadherin and smaller mitochondria, with diffuse cristae. Lack of β-cell K8 also leads to a reduced glucose stimulated insulin secretion response in vivo, despite undisturbed systemic blood glucose regulation. K8flox/flox; Ins-Cre mice have a decreased sensitivity to STZ compared to K8 wild-type mice, which is in line with decreased membranous GLUT2 expression observed in vivo, as GLUT2 is required for STZ uptake in β-cells. In vitro, MIN6 cell plasma membrane GLUT2 is rescued in cells overexpressing K8/K18 filaments, but mistargeted in cells with disrupted K8/K18 filaments. β-cell K8 is required for islet and β-cell structural integrity, normal mitochondrial morphology and GLUT2 plasma membrane targeting, and has implications on STZ sensitivity as well as systemic insulin responses.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

The keratin-desmosome scaffold of internal epithelia in health and disease – The plot is thickening

Cited by 2 publications

References 57 publications

Identification and culture of functional salivary gland ductal epithelial cells

Identification and culture of functional salivary gland ductal epithelial cells

β-Cell keratin 8 maintains islet mechanical integrity, mitochondrial ultrastructure, and β-cell glucose transporter 2 plasma membrane targeting

Contact Info

Product

Resources

About